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Blood DNA conserving card and method for making the same

A technology for storing cards and blood, applied in the biological field, can solve the problems of lack of DNA separation and purification conditions, limited storage time, poor protection, etc., and achieve the effects of good safety, long storage time, and high safety protection.

Active Publication Date: 2011-08-31
DINGSHENG TECH BEIJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of method 2 is that it is convenient for collection, storage and transportation. The disadvantage is that the protection is poor. If there is a highly infectious virus in the collected blood, it will cause harm to the surrounding people. Severe degradation of sample DNA
Method 3 Direct preservation of DNA can maximize the preservation time, but often the collection site does not have the conditions for DNA separation and purification

Method used

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  • Blood DNA conserving card and method for making the same
  • Blood DNA conserving card and method for making the same

Examples

Experimental program
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Effect test

Embodiment 1

[0032] The making of embodiment 1 blood DNA preservation card:

[0033] 1) Dissolve 3 grams of Tris base and 3.72 grams of EDTANa in 300ml of deionized water 2 2H20, 25 grams of SDS, 118 grams of guanidine isothiocyanate and 1.9 mg of thiourea, adjust the pH to 8.0 with concentrated hydrochloric acid, add deionized water to make up to 500ml;

[0034] 2) Cut a nitrocellulose membrane of a certain size, with a thickness of about 0.3 mm, and put it into a certain volume of the above solution (according to 50 μl solution / cm 2 medium ratio), placed in a closed container to prevent external DNA contamination, and shaken at 100 rpm / min for 30 minutes until the solution was completely absorbed.

[0035] 3) Carefully take out the card that has absorbed the salt solution, bake at 80°C for 1 hour, and fully dry.

Embodiment 2

[0037] 1) Dissolve 3 grams of TrisBase and 1.86 grams of EDTANa in 300ml of deionized water 2 2H 2 0, 10 grams of SDS, 59 grams of guanidine isothiocyanate and 0.95 mg of thiourea, adjust the pH to 8.0 with concentrated hydrochloric acid, add deionized water to make up to 500ml;

[0038] 2) Cut a filter paper medium of a certain size with a thickness of about 1 mm, put it into a certain volume of the above solution (according to the ratio of 50 μl solution / cm2 medium), place it in a closed container to prevent external DNA contamination, shake at 100 rpm / min for 60 minutes, until The solution is absorbed completely.

[0039] 3) Carefully take out the card that has absorbed the salt solution, bake at 80°C for 1 hour, and fully dry.

Embodiment 3

[0041] 1) Dissolve 5 grams of TrisBase, 2.75 grams of EDTANa2 2H20, 15 grams of SDS, 80 grams of guanidine isothiocyanate and 1.29 mg of thiourea in 300 ml of deionized water, adjust the pH to 8.0 with concentrated hydrochloric acid, add deionized water to make up to 500ml;

[0042] 2) Cut a certain size of silica gel membrane medium with a thickness of about 0.5mm, put it into a certain volume of the above solution (according to the ratio of 50μl solution / cm2 medium), place it in a closed container to prevent external DNA contamination, shake at 100rpm / min for 40 minutes until the solution is completely absorbed.

[0043] 3) Carefully take out the card that has absorbed the salt solution, bake at 80°C for 1 hour, and fully dry.

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Abstract

The invention relates to 'a blood DNA preserving card and production method' and belongs to the biology technical field. A blood DNA preserving card comprises fiber-shaped media with the ability of absorbing DNA, and is characterized in that: the salinity is absorbed in the media and contains cell lysing reagent, nuclease inhibitor, a substance allowing the pathogenic bacterium and the virus protein to be denaturalized as well as anti-oxidization inhibitor. These salinities can inhibit the activities of the viruses to prevent the infection, can prevent the nuclease from gradating DNA, so thatthe blood DNA can be preserved indoor for a long-term. The pure DNA can be obtained from the preserved DNA in the card of the invention by bleaching the salt ions and blood corpuscle leftovers on thecard media with the water, thereby avoiding the extraction process, ensuring the operation is simpler, being convenient to be operated in batches and saving the expense of the DNA extraction agent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blood DNA preservation card. Background technique [0002] With the development of genomics research, DNA analysis has gradually become a basic means in the fields of disease diagnosis, genetic analysis and individual identification. The main source of human DNA is blood, which has important practical significance for the collection, transportation and preservation of blood or DNA samples in blood. [0003] At present, there are mainly several types of conventional storage and transportation of blood or blood DNA: 1. Take venous blood, add anticoagulant to the blood, and store and transport it in a liquid state at low temperature; 2. Collect finger blood and apply it to filter paper. Good adsorption, can be combined with blood cells, easy to store; 3, DNA in the blood is separated on site after blood collection, and stored. [0004] The above-mentioned three methods have great li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12M1/00C12N15/10C12N5/08
Inventor 高阳陈初光马斌王曙光
Owner DINGSHENG TECH BEIJING
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