Microorganism catalytic synthesis process for mesoporous nano powder body hydroxyapatite

A technology of nano-hydroxyapatite and hydroxyapatite, applied in the direction of fermentation, etc., can solve the problems of complex preparation process, low activity, high cost, etc., achieve strong hydrophobicity and bio-affinity, simple preparation process, and low cost Effect

Inactive Publication Date: 2010-08-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the particles prepared by the existing method have serious agglomeration, low activity, complex preparation process and high cost, poor application effect, and are difficult to popularize and apply.
The synthesis method of HAP ultrafine powder has made some progress in the world, but most of the methods are still in the laboratory stage. How to choose different preparation conditions, control the shape and particle size of HAp powder, and carry out mass production to meet Biomedical demand for hydroxyapatite ultrafine powder is still a direction that requires a large number of scientific workers

Method used

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  • Microorganism catalytic synthesis process for mesoporous nano powder body hydroxyapatite
  • Microorganism catalytic synthesis process for mesoporous nano powder body hydroxyapatite
  • Microorganism catalytic synthesis process for mesoporous nano powder body hydroxyapatite

Examples

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Effect test

Embodiment 1

[0027] Example 1: 150 ml of 25 g / L glycolipid-based biosurfactant Coccidia sophorolipid aqueous solution was added at room temperature with 6 g of sugar additives, stirred at room temperature for 20 minutes, and emulsified at 25 ° C for 50 minutes to obtain fermented emulsion A , and then, 50ml of 0.5mol / L CaCl 2 The solution was added to emulsion A, and magnetic stirring was continued for 30 minutes to allow Ca 2+ It can be fully adsorbed in the nanovesicle structure of microbial cells. Slowly add 0.5 mol / L phosphoric acid solution dropwise at a rate of 10 drops per minute, and make Ca / P (molar ratio) = 1.67, adjust pH = 10 with 5 mol / L NaOH solution, continue magnetic stirring for 0.5 h, let the cell structure part Mineralize the sediment, then let it stand for 20 hours, centrifuge at 5000 rpm for 5 minutes, wash twice with water and wash once with alcohol to remove Cl - 、Na + and H 2 O. Finally, the precipitate was dried in a drying oven at 70°C for 20 hours to obtain ...

Embodiment 2

[0028] Example 2: 150ml of 50g / L glycolipid-based biosurfactant rhamnolipid aqueous solution was added at room temperature with 4.5g of sugar additives, stirred at room temperature for 50 minutes, and emulsified at 35°C for 30 minutes to obtain fermented emulsion A. Then 1.65g Ca(OH) 2 Add the powder to the emulsion A, continue magnetic stirring for 50 minutes, let the Ca 2+ It can be fully adsorbed in the nanovesicle structure of microbial cells. Slowly add 0.3 mol / L ammonium dihydrogen phosphate solution dropwise at a speed of 20 drops per minute, and make Ca / P (molar ratio) = 1.67, continue magnetic stirring for 1 h, allow the mineralization and deposition of the cell structure part, then let stand for 15 h, and Centrifuge at 5000 rpm for 3 minutes, wash 3 times with water and wash 2 times with alcohol to remove OH- and NH 4 + and H 2 O. Finally, dry the precipitate in a drying oven at 80°C for 24 hours to obtain a mesoporous HAp / protein nanocomposite powder sample with ...

Embodiment 3

[0035] Example 3: 150ml of 20g / L glycolipid-based biosurfactant trehalose lipid aqueous solution was stirred at room temperature with 9g of sugar additives, stirred at room temperature for 30 minutes, and emulsified at 20°C for 1 hour to obtain emulsion A, and then 40ml of 0.6mol / L Ca(NO 3 ) 2 Add the solution to Emulsion A and continue magnetic stirring for 1 hour to allow Ca 2+ It can be fully adsorbed in the nanovesicle structure of microbial cells. Slowly add 0.6mol / L sodium phosphate solution dropwise at a speed of 15 drops per minute, and make Ca / P (molar ratio)=1.8, adjust pH=12 with 5mol / L NaOH solution, continue magnetic stirring for 45 minutes, let the cell structure Partially mineralize and deposit, then stand still for 18 hours, high-speed centrifuge (5000 rpm, 10 minutes), wash twice with water and wash once with alcohol to remove NO 3 - and Na + and H 2 O. Finally, the precipitate was dried in a drying oven at 90°C for 30 hours to obtain a mesoporous HAp / p...

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Abstract

The invention relates to a microorganism catalytic synthetic method for mesoporous nanometer powder of hydroxyapatite, which comprises the following procedures that: surfactant of natural microorganism and sugar aid are added into water, the mixture is stirred and cultivated to form fermented emulsifying liquid, which is added with liquid of inorganic salt with calcium, stirred, and added with liquid of inorganic salt with phosphorous, the molecular ratio between calcium and phosphorous is kept between 1.6-1.8, and the pH is adjusted to be 9-12, then the mixture is stirred and centrifuged, the deposit after centrifuge is washed with water or ethanol, or the separation liquid obtained through centrifuge is treated again according to the procedures as above, the obtained deposit is dried and heated, with the temperature being preserved, to obtain the mesoporous nanometer powder of hydroxyapatite. The synthetic method provided by the invention has a simple technique and low cost without pollution, the prepared sample of nanometer synthetic powder has the open spiral structure of biological skeleton and the ordered mesoporous structure with helminth fissure, without the need to demolish the mold, while the granules are positively charged on the surface, with the effect of laevorotatory light, strong hydrophobicity, and cellular affinity, without toxic effect or aide effect.

Description

technical field [0001] The invention relates to a microbial catalytic synthesis method of mesoporous nano powder hydroxyapatite (HAp). Background technique [0002] In recent years, there have been many reports on the preparation methods of nano-hydroxyapatite, mainly including dry synthesis and wet synthesis. The most widely used laboratory synthesis method is wet synthesis, mainly including precipitation method, sol-gel method, hydrothermal reaction method, template method and so on. However, the particles prepared by the existing method have serious agglomeration, low activity, complex preparation process and high cost, poor application effect, and are difficult to popularize and apply. The synthesis method of HAP ultrafine powder has made some progress in the world, but most of the methods are still in the laboratory stage. How to choose different preparation conditions, control the shape and particle size of HAp powder, and carry out mass production to meet The demand...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00
Inventor 王迎军何文魏坤陈晓峰尹诗衡郑华德陈景帝
Owner SOUTH CHINA UNIV OF TECH
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