Tibetan medicine bongga extraction and its application in preparing antiviral medicine
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A technology of antiviral drugs and extracts, applied in antiviral agents, applications, food preparation, etc., can solve the problems of unseen antibacterial and antiviral effects research
Active Publication Date: 2008-04-30
LANZHOU UNIVERSITY
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[0005] Looking at the reports at home and abroad, there is no research on the antibacterial and antiviral effects of the total ethanol extract, volatile oil and alkaloid extracts of the Tibetan medicine Bangga
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Embodiment 1
[0018] Example 1 Antibacterial effect of Tibetan medicine Bangga extract
[0019] 1. Strain and culture medium
[0020] The experimental strains were provided by Gansu Provincial Center for Disease Control and Gansu Provincial Clinical Laboratory Center. The list is as follows:
[0029] Experimental medium: Bacterial MH medium and fungal PDA medium were purchased from China Institute for the Control of Pharmaceutical and Biological Products.
[0030] 2 Bacteriostatic effect detection method:
[0031] 2.1 Antibacterial effect Determination of the size of the antibacterial zone by the cup and saucer ...
Embodiment 2
[0043] Embodiment 2: Antiviral effect of Tibetan medicine Bangga extract
[0044] 1. Experimental materials
[0045] Virus: HSV-II type (3rd generation) 20060913, provided by the Vaccine Laboratory of Lanzhou Institute of Biological Products (herpessiplex virus, HSV-II)
[0046] Cells: African green monkey kidney cells (Vero cells) provided by Lanzhou Institute of Biological Products. Cultured with DMEM medium + 7% bovine serum.
[0047] Animals: Clean-grade Kunming mice, half male and half female, weighing 18-22 g, were purchased from the animal room of Lanzhou Institute of Biological Products.
[0048] Reagents: thiazolium blue (MTT, Fluka Biochemika Company), dimethylsulfoxide (dimethylsulfoxide, DMSO, produced by Sigma Company), DMEM (GIBCO Company), standard newborn bovine serum (Sino-US joint venture Lanzhou Minhai Bioengineering Co., Ltd.), Acyclovir for injection (ACV, 9-(2-hydroxyethoxymethyl-)guanine, Wuhan Pusheng Pharmaceutical Co., Ltd., production batch number 0...
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Abstract
The invention relates to a banga extract and the application in preparing antiviral medicine, which can not only inhibit various etiological agent bacteria, but also can play evident antiviral function through a plurality of connections such as inhibiting virus absorption, directly killing living virus, and inhibiting proliferation of virus, thereby the extract can be applied in preparing medicine which resists bacteria and virus, and simultaneously can be applied in food, health products and cosmetics. The banga extract of the invention can be made into medicine compound in preparing of the medicine which resists bacteria and virus in single-flavor or compound style, in particular to the application of the single-flavor or the compound style in preparing medicine compound for curing genital herpes. Simultaneously, the extract can be used incorporated in food, health products and cosmetics. The banga extract has the advantages of rich raw material, low cost, simple extraction technique, and can be conveniently shaped into various shapes, and has important developing and applying prospect.
Description
technical field [0001] The invention relates to the extract of Tibetan medicine Bangga and the application of the extract in the preparation of antibacterial and antiviral drugs, belonging to the field of ethnopharmacy. Background technique [0002] The increasing bacterial resistance caused by the extensive use of antibiotics has become one of the problems of common concern all over the world. Especially the drug resistance rate of Staphylococcus aureus has reached more than 90%. Herpes simplex virus type II mainly causes skin and mucous membrane infection in the genital area. The virus enters the body through the respiratory tract, oral cavity, genital mucosa and damaged skin, and resides in the normal mucosa, blood, saliva and sensory ganglion cells of the human body. When the body's resistance declines, such as fever, gastrointestinal dysfunction, menstruation, pregnancy, focal infection, and emotional changes, the latent HSV-II in the body is activated to cause diseas...
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