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Method for preparing ethanol and yeast superoxide dismutase from sweet sorghum stem liquor

A technology for superoxide and ethanol production, applied in the field of chemical engineering, can solve problems such as low yield, and achieve the effects of high ethanol yield and cost reduction

Inactive Publication Date: 2010-08-18
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This technology can only get one product of SOD, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Measure the sterilized sweet sorghum stalk juice (total sugar content is 12%), add (NH) under aseptic conditions 2 SO 4 20g·L -1 , K 2 HPO 4 2.0g·L -1 , MgSO 4 ·7H 2 O 0.5g·L -1 , Zn(CH 3 COO) 2 ·2H 2 O5.0mg·L -1 , CuSO 4 ·5H 2 O 12mg·L -1 ; Use 6mol·L -1 Adjust the pH value of the HCl sterile solution to 4.0; insert the yeast species-Saccharomyces cerevisiae at 10% (v / v) of the total volume of the fermentation broth. At 30℃, 110r·min -1 Fermentation at a stirring rate. After the ethanol fermentation is over, the fermentation broth is heated to 5℃, 5000r·min -1 Centrifuge for 15 min, and take the supernatant for distillation to extract ethanol. Wash the precipitated bacteria once with sterile water, 3000r·min -1 Centrifuge at room temperature to collect the bacteria. Add the cell activation solution at 10% (w / w) of the wet cell weight, and place it in a 30℃ water bath for 120r·min -1 After shaking and activating for 60 minutes, use conventional methods to destroy the y...

Embodiment 2

[0045] Measure the sterilized sweet sorghum stalk juice (total sugar content is 12%), add (NH) under aseptic conditions 2 SO 4 25g·L -1 , K 2 HPO 4 2.5g·L -1 , MgSO 4 ·7H 2 O 0.65g·L -1 , Zn(CH 3 COO) 2 ·2H 2 O5.7mg·L -1 , CuSO 4 ·5H 2 O 12mg·L -1 ; Use 6mol·L -1 Adjust the pH value of the HCl sterile solution to 4.0; insert the yeast species-Saccharomyces cerevisiae at 10% (v / v) of the total volume of the fermentation broth. At 30℃, 130r·min -1 Fermentation at a stirring rate. After the fermentation, the fermentation broth is heated to 7℃, 3000r·min -1 Centrifuge for 15 min, and take the supernatant for alcohol distillation. Wash the bacteria twice with sterile water, 3000r·min -1 Centrifuge at room temperature to collect the bacteria. Add the cell activation solution at 10% (w / w) of the wet cell weight, and place it in a 30℃ water bath for 120r·min -1 After shaking and activation for 30 minutes, use conventional methods to destroy the yeast cell wall, and add 15mL pH7.8 phosp...

Embodiment 3

[0047] Measure the sterilized sweet sorghum stalk juice (total sugar content is 15%), add (NH) under aseptic conditions 2 SO 4 22.5g·L -1 , K 2 HPO 4 2.0g·L -1 , MgSO 4 ·7H 2 O 0.6g·L -1 , Zn(CH 3 COO) 2 ·2H 2 O5.0mg·L -1 , CuSO 4 ·5H 2 O 15mg·L -1 ; Use 6mol·L -1 Adjust the pH value of the HCl sterile solution to 5.0; add 15% (v / v) of the total volume of the fermentation broth to the yeast species-Saccharomyces cerevisiae. At 35℃, 150r·min -1 Fermentation at a stirring rate. After the fermentation is over, put the fermentation broth at 5℃, 5000r·min -1 Centrifuge for 15 min, and take the supernatant for alcohol distillation. Wash the bacteria twice with sterile water, 3000r·min -1 Centrifuge at room temperature to collect the bacteria. Add the cell activation solution at 10% (w / w) of the wet cell weight, and place it in a 30℃ water bath for 120r·min -1 After shaking and activating for 60 minutes, use conventional methods to destroy the yeast cell wall, and add 15 mL of pH 7.8 ...

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PUM

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Abstract

The invention relates to a method for producing ethyl alcohol and yeast superoxide dismutase by using juice of stems of sweet sorghum, and belongs to the technical field of the biomaterial energy engineering. The invention has the following steps: firstly, the raw material of the juice of the stems of the sweet sorghum and fermentation auxiliary materials are taken to be sterilized and cooled forstandup; secondly, saccharomyces cervisiae is taken to be augmented stage by stage and cultured until the cell number per milliliter achieves above 1.0*10<8>; thirdly, the juice of the stems of the sweet sorghum and the fermentation auxiliary materials are mixed, and filled with the saccharomyces cerivisiae to perform ethyl alcohol fermentation; fourthly, after the ethyl alcohol fermentation is finished, refrigerated centrifugation to the fermentation liquid is performed, the supernatant fluid is taken to be used for distilling and extracting the ethyl alcohol, and the thallus is used for extracting the SOD; fifthly, after the collected thallus is activated, the cell wall of microzyme is damaged, and is heat insulated and extracted by phosphate buffer solution, then solid liquid separation is performed, and the obtained clear liquid is yeast SOD extracting solution. The yield rate of the ethyl alcohol can achieve 90.3 to 97.03 percent, and the output of the superoxide dismutase in each liter of fermentation liquid is 3.64*10<4>U to 6.95*10<4>U.

Description

Technical field [0001] The invention relates to a method in the technical field of chemical engineering, in particular to a method for preparing ethanol and yeast superoxide dismutase from sweet sorghum stalk juice. Background technique [0002] Sweet sorghum is a C4 crop variety with high biological yield, per hm 2 Sweet sorghum can generally produce 2250-7500kg of grain and 55-75t stalks rich in sugar (15°-20°Brix). The stalk has a sugar content of 15°-20°Brix and a juice yield of more than 55%. It is suitable for the use of liquid fermentation technology to convert ethanol into liquid fuel. It has broad prospects in the production of fuel ethanol and is known as the 21st century "Green Energy". Relevant researchers have used the main goal of fermentation ethanol yield to carry out ethanol fermentation conditions such as the addition of nitrogen sources, nutrients, and the initial sugar concentration of the fermentation broth, the amount of inoculation, temperature, and the nu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 刘荣厚梅晓岩
Owner SHANGHAI JIAOTONG UNIV
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