Method for extracting and purifying vitexin and isoviextin from cajanus cajan branches and leaves

Abstract

The invention relates to an extraction and purification method of the component with the Chinese herbal medicine functionality, and aims at providing a method of obtaining monomeric compound Vitexin and Isovitexin through extraction and purification from pigeon pea branches and leaves in a simple, safe, economical and high-efficient manner. The invention has the technical proposal of using the fresh and dried pigeon pea branches and the leaves as raw materials, and adopting the combination of a homogenate extraction technique, an ultrasonic assistance extraction technique, an ultrasonic oscillation flocculation technique, a negative pressure cavitation suspension extraction technique, a macropore absorbing resin collecting technique, an ODS-C18 reversed phase medium pressing silicagel column chromatography technique, a low temperature recrystallization and a recrystallization technique, and then the Vitexin and the Isovitexin with the purity of more than 90 percent are obtained, and the extraction rate is more than 0.17 percent and 0.13 percent respectively. The raw material used by the invention is the wasted fresh or dried pigeon pea branches and leaves in the agricultural production, thus the invention cannot damage the ecological balance of plants and affect the agricultural production, and can further get the pigeon pea functionality component. Moreover, the method has the steps of extraction, primary decontaminating and purification, and is simple and practical with little objective compound, thus the method is applicable for the industry application, and has important meaning to the industrialized production.

Description

technical field [0001] The invention relates to a method for separating and purifying functional components from plants, in particular extracting and purifying vitexin and isovitexin from fresh or dry branches and leaves of pigeonpea. Background technique [0002] Pigeon pea [Cajanus cajan (L.) Millsp.] is an annual or perennial woody plant and evergreen shrub of the genus Fabaceae. Also known as pigeon beans, tree soybeans, etc., it is a folk medicine. It has cooling, heat-reducing, detoxifying, analgesic, hemostatic, antibacterial and anti-inflammatory effects. It is used in folks to treat trauma, burns, infections, bedsores, antibacterial and anti-inflammatory, etc. (Liu Zhongqiu et al., 1998; Sun Shaomei et al., 1995). In addition, pigeonpea extract preparations are used in India, Brazil, Cuba, Mexico, South Africa and other countries to treat colds, bronchitis, measles, dysentery, hepatitis, skin diseases, burns, ulcers, tumors and other diseases (Abbiw DK., 1990; Duk...

AI Technical Summary

Problems solved by technology

The traditional purification process is long and the loss of the target compound is large. Multiple silica gel column chromatography is used. The operation steps are numerous and the eluent is mainly toxic and expensive reagents such as chloroform, acetone, and ethyl acetate.

In the Chinese patent "Rapid Preparation Method of Several High-purity Medicinal Substances in Patrinia dahliae" (publication number CN1683386), isovitexin was prepared by traditional liquid-liquid extraction, column chromatography and high-efficiency countercurrent chromatography. Long, large amount of organic solvents, and restricted by instruments and equipment

Lin Li (1999) used traditional column chromatography to separate vitexin and isovitexin, which took a long time and the yield was low

[0005] In summary, in the process of using plant resources to separate and purify vitexin and isovitexin, the main influencing factors are the backward extraction process and the widespread loss of vitexin and isovitexin in the process of raw material treatment and separation , The purification process is long, which increases the cost

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