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High-concentration alcoholic fermentation method

A technology of alcohol fermentation and high concentration, applied in the field of bioengineering, can solve the problem of high cost of accelerators, achieve good economic benefits, simplify procedures, and achieve high alcohol concentration

Inactive Publication Date: 2012-05-23
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And some accelerators are expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Saccharomyces cerevisiae SY-1 screened by our laboratory was used as the strain for fermentation. The strain was preserved in China Center for Type Culture Collection on October 23, 2006, with the preservation number CCTCC M 206111.

[0030] (1) The total sugar in the fermentation medium is 305g L -1 , and the remaining ingredients are (g L -1 ): yeast powder 5, peptone 5, (NH 4 )2 SO 4 1.5, KH 2 PO 4 1.5, MgSO 4 .7H 2 O 0.6, CaCl 2 3.0, pH6.0~6.5. The amount of accelerator added is (mg L -1 ): V B1 10,V B6 30, niacin 30, p-aminobenzoic acid 5. Sterilize at 0.08MPa for 15 minutes. Into sterile air, dissolved oxygen 90%. Inoculation, 30°C, 200r / min shaking table shaking fermentation. During the fermentation, feed was fed twice, and the time was 8h and 24h respectively. The fermentation time is 54h. The final ethanol concentration was 18.12% (20°C, V / V, the same below), the fermentation efficiency was 91.73%, and the residual sugar was 0.25% (g / 1...

Embodiment 2

[0036] Example 2: Fermentation with Saccharomyces cerevisiae as the strain, and the fermentation volume is 1 L.

[0037] (1) Activate the dry yeast and inoculate it in the medium for fermentation. The total sugar in the fermentation medium is 295gL -1 , all the other ingredients are with embodiment 1 (1). Into sterile air, dissolved oxygen 65%. During the fermentation, feed was fed three times, and the time was 8h, 15h, and 24h respectively. Fermentation time 60h. The final ethanol concentration was 17.47%, the residual sugar was 0.39%, and the fermentation efficiency was 91.44%.

[0038] (2) The sugar clear liquid obtained after cassava starch liquefaction and saccharification is used as carbon source. Total sugar is 275g L -1 , fermentation condition is the same as embodiment 1 (6), ferments to 54h and ends. The final ethanol concentration was 16.15%, the fermentation efficiency was 90.68%, the residual reducing sugar was 0.45%, and the residual total sugar was 0.89%....

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PUM

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Abstract

The invention pertains to the bio-engineering technical field, disclosing a fermentation method of alcohol with high concentration. The invention overcomes the shortages of low fermentation efficiency and long fermentation time, etc., of the existing alcohol fermentation technique. The invention adopts the steps that: bacteria-free air is introduced at the first stage; after 8 to 30 hours fermentation, material and fermentation agent are added under the temperature of 25 to 35 DEG C, oscillated or stirred with a rotation speed of 10-500r / min for 50 to 66 hours; the high concentration alcohol is made, and the volume concentration of the alcohol in the fermentation liquid is up to 16 to 18 percent. The invention can fast and effectively produce the high concentration alcohol.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a method for fermenting and producing high-concentration alcohol, which can produce high-concentration alcohol quickly and efficiently. Background technique [0002] With the advent of the energy crisis, the use of renewable resources (food or plant fiber) to ferment and produce ethanol as a bioenergy to replace or partially replace petroleum has been put on the agenda. Fuel ethanol, as a clean energy source, has attracted much attention in today's world of increasing emphasis on environmental protection. [0003] At present, using starchy raw materials as substrates, alcohol factories in the United States can reach 12% (V / V, the same below). However, my country's fuel ethanol production technology is mainly based on starchy alcohol production technology. The ethanol content in the fermentation liquid is 8% to 10%, which is lower than the world level, and the ethanol ferm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 赵海刘艳戚天胜
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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