Saccharomyces cerevisiae and its application in preparation of beta-hydroxyphenyl propionic acid ethyl
A technology of ethyl carbonyl phenylpropionate and Saccharomyces cerevisiae strain, which is applied in the directions of fermentation, fungi, etc., can solve the problems of low production capacity of biotransformation, cytotoxicity, small contact opportunity of ethyl β-carbonyl phenylpropionate, etc. Easy to large-scale industrial production, low cost, and the effect of reducing toxic effects
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Embodiment 1
[0037] Slant culture: Inoculate CGMCC No.2230 bacteria into a culture medium containing wort juice 10g / L, yeast powder 3g / L, peptone 5g / L, glucose 10g / L, agar 20g / L, and natural pH. Cultivate at 30°C for 4-6 days.
[0038] Seed cultivation and fermentation: Both the seed and fermentation medium use liquid medium, the composition is glucose 30g / L, yeast powder 3g / L, ammonium sulfate 5g / L, anhydrous MgSO 4 0.25g / L, K 2 HPO 4 ·3H 2 O 1g / L, KH 2 PO 4 1g / L. Use an inoculation needle to take a ring of bacteria from the slant culture medium and inoculate it into a 250ml Erlenmeyer flask containing 100ml of liquid medium, and culture it at 30°C and 180r / min for 24h to obtain seeds. Inoculate the seed solution with a 10% inoculum amount into a 250ml Erlenmeyer flask containing 100ml of liquid medium, cultivate it at 30°C and 180r / min for 24 hours to obtain a fermented liquid, and use the obtained fermented liquid to immobilize the bacteria .
[0039] Mix 5 bottles of 100ml fe...
Embodiment 2
[0045] CGMCC No.2230 was cultivated according to the method in Example 1 to obtain bacterial cell fermentation broth. 4 bottles of 100ml fermented broth with a dry weight of 430mg were mixed with 2% sodium alginate solution at an equal volume concentration to make a mixed solution, and the mixed solution was respectively packed into syringes with different needle sizes and dripped with 3.5% ( w / v) CaCl 2 Immobilized particles were formed in the solution, and the diameters of the formed particles were 2mm, 3mm, 4mm and 5mm respectively. They were solidified at 37°C for 30 minutes, and the obtained immobilized particles were washed with sterile physiological saline to remove excess calcium ions and untreated particles. For the captured cells, the obtained immobilized cell particles were continued to proliferate and culture in liquid medium for 24 hours.
[0046] Add the 4 kinds of immobilized cell granules that have been proliferated and cultured into the reaction system of 300...
Embodiment 3
[0051] CGMCC No.2230 was cultivated according to the method in Example 1 to obtain bacterial cell fermentation broth. Mix 4 bottles of fermented broth with a dry weight of 430 mg of bacteria and 2% sodium alginate solution at an equal volume concentration to make a mixed solution, put the mixed solution into a syringe, and drop 3.5% (w / v) CaCl 2 Form immobilized particles in the solution, the diameter of the formed immobilized particles is 2mm, solidify at 37°C for 30min, and the obtained immobilized particles are washed with sterile physiological saline to remove excess calcium ions and uncaptured cells, and the obtained 4 The bottle-immobilized cell particles continued to proliferate and culture in liquid medium for 0h, 24h, 48h and 72h respectively.
[0052] Add 4 kinds of immobilized cell granules that have been proliferated and cultured into the reaction system of 300ml water / dibutyl phthalate (volume ratio 1:1), add 15.45mmol ethyl β-carbonylphenylpropionate, and heat at...
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