Saccharomyces cerevisiae and its application in preparation of beta-hydroxyphenyl propionic acid ethyl

A technology of ethyl carbonyl phenylpropionate and Saccharomyces cerevisiae strain, which is applied in the directions of fermentation, fungi, etc., can solve the problems of low production capacity of biotransformation, cytotoxicity, small contact opportunity of ethyl β-carbonyl phenylpropionate, etc. Easy to large-scale industrial production, low cost, and the effect of reducing toxic effects

Inactive Publication Date: 2011-11-09
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extremely small solubility of the organic substrate ethyl β-carbonylphenylpropionate in water, the chance of contact between ethyl β-carbonylphenylpropionate and cells is very small, and the organic substrate will also cause certain poisonous effects on cells, so biological Conversion productivity is low

Method used

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  • Saccharomyces cerevisiae and its application in preparation of beta-hydroxyphenyl propionic acid ethyl
  • Saccharomyces cerevisiae and its application in preparation of beta-hydroxyphenyl propionic acid ethyl

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Slant culture: Inoculate CGMCC No.2230 bacteria into a culture medium containing wort juice 10g / L, yeast powder 3g / L, peptone 5g / L, glucose 10g / L, agar 20g / L, and natural pH. Cultivate at 30°C for 4-6 days.

[0038] Seed cultivation and fermentation: Both the seed and fermentation medium use liquid medium, the composition is glucose 30g / L, yeast powder 3g / L, ammonium sulfate 5g / L, anhydrous MgSO 4 0.25g / L, K 2 HPO 4 ·3H 2 O 1g / L, KH 2 PO 4 1g / L. Use an inoculation needle to take a ring of bacteria from the slant culture medium and inoculate it into a 250ml Erlenmeyer flask containing 100ml of liquid medium, and culture it at 30°C and 180r / min for 24h to obtain seeds. Inoculate the seed solution with a 10% inoculum amount into a 250ml Erlenmeyer flask containing 100ml of liquid medium, cultivate it at 30°C and 180r / min for 24 hours to obtain a fermented liquid, and use the obtained fermented liquid to immobilize the bacteria .

[0039] Mix 5 bottles of 100ml fe...

Embodiment 2

[0045] CGMCC No.2230 was cultivated according to the method in Example 1 to obtain bacterial cell fermentation broth. 4 bottles of 100ml fermented broth with a dry weight of 430mg were mixed with 2% sodium alginate solution at an equal volume concentration to make a mixed solution, and the mixed solution was respectively packed into syringes with different needle sizes and dripped with 3.5% ( w / v) CaCl 2 Immobilized particles were formed in the solution, and the diameters of the formed particles were 2mm, 3mm, 4mm and 5mm respectively. They were solidified at 37°C for 30 minutes, and the obtained immobilized particles were washed with sterile physiological saline to remove excess calcium ions and untreated particles. For the captured cells, the obtained immobilized cell particles were continued to proliferate and culture in liquid medium for 24 hours.

[0046] Add the 4 kinds of immobilized cell granules that have been proliferated and cultured into the reaction system of 300...

Embodiment 3

[0051] CGMCC No.2230 was cultivated according to the method in Example 1 to obtain bacterial cell fermentation broth. Mix 4 bottles of fermented broth with a dry weight of 430 mg of bacteria and 2% sodium alginate solution at an equal volume concentration to make a mixed solution, put the mixed solution into a syringe, and drop 3.5% (w / v) CaCl 2 Form immobilized particles in the solution, the diameter of the formed immobilized particles is 2mm, solidify at 37°C for 30min, and the obtained immobilized particles are washed with sterile physiological saline to remove excess calcium ions and uncaptured cells, and the obtained 4 The bottle-immobilized cell particles continued to proliferate and culture in liquid medium for 0h, 24h, 48h and 72h respectively.

[0052] Add 4 kinds of immobilized cell granules that have been proliferated and cultured into the reaction system of 300ml water / dibutyl phthalate (volume ratio 1:1), add 15.45mmol ethyl β-carbonylphenylpropionate, and heat at...

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Abstract

The invention discloses a novel microbial strain Saccharomyces cerevisiae CGMCC No.2230 and application of the Saccharomyces cerevisiae CGMCC No.2230 in biotransformation preparation of beta-hydroxybenzoic ethyl propionate, wherein, the Saccharomyces cerevisiae CGMCC No.2230 is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms with a collection number of CGMCC No.2230. The application of the invention is that: beta-carbonyl benzoic ethyl propionates are taken as substrates, and immobilized cell particles which are prepared by fermentation broth obtained after fermentation of the Saccharomyces cerevisiae strain CGMCC No.2230 are taken as biocatalysts; transformation reaction time of transformation liquid is 8 to72 hours at the temperature of 25 to 30 DEG C in a water / dibutyl phthalate two-phase system, and the product beta-hydroxybenzoic ethyl propionates are obtained after separation and purification of the transformation liquid. The microbial transformation method has the advantages of friendly surroundings, mild reaction conditions, simple product separation, high substrate transformation ratio, good product purity and biocatalysts capable of being reused, and is suitable for commercial process.

Description

technical field [0001] The invention relates to a new microbial strain, Saccharomyces cerevisiae (Saccharomyces cerevisiae) CGMCC No.2230, and its application in the preparation of ethyl β-hydroxyphenylpropionate through microbial transformation. Background technique [0002] Beta-hydroxy-benzenepropanoic acid ethylester, the molecular formula is C 11 h 14 o 3 , molecular weight 194.23. Ethyl β-hydroxyphenylpropionate is an important intermediate in the synthesis of antidepressant drugs, which can be used in the synthesis of antidepressant drugs atomoxetine, fluoxetine and nisoxetine, respectively. Among them, fluoxetine is the first-line antidepressant drug. The "World Health Report" pointed out: At present, depression has become the fourth largest chronic disease in the world after cancer, stroke and chronic obstructive pulmonary disease, and has become a serious public health problem that cannot be ignored. Affected by intensified social competition and aging, the in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P7/62
Inventor 欧志敏杨根生应国清
Owner ZHEJIANG UNIV OF TECH
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