Method for extracting flavone and polysaccharide from mulberry leaf
A technology for flavonoids and mulberry leaves, applied in the field of extracting flavonoids and polysaccharides, can solve the problems of low yield of active ingredients, low selectivity, long operation time, etc. Effect
Inactive Publication Date: 2008-07-09
ZHEJIANG GONGSHANG UNIVERSITY
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The present invention discloses a method of extracting flavone and polyoses from mulberry leaves, including air drying, grinding and screening. Mulberry leaf powder is immersed in 60 to 80 percent ethanol so as to extract flavone or in water so as to extract polyoses; the ultrasonic treatment with 100 to 400W power lasts for 5 to 25 minutes; extraction temperature is controlled within 60 to 85 DEG C and the extraction lasts for 1 to 2 hours; the extracting solution is centrifugalized and filtered by supernatant fluid, thus obtaining the filtrate; the flavone filtrate is concentrated so as to obtain the mulberry leaf flavone extract; or ethanol is added into the polyoses filtrate so as to make the ethanol concentration up to 70 percent in the final solution; the filtrate is placed for 18 to 24 hours under 4 to 10 DEG C, then deposited and dried, thus obtaining the mulberry leaf polyoses. The flavone yield can reach more than 25mg/g and the polyoses yield 110mg/g. The present invention, with the advantages of good repeatability, high selection, short operating time, less solvent consumption, high yield of effective ingredients, no noise, no pollution and relatively good application prospective, is applicable to industrial production.
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