Method for producing succinic acid by fermenting straw raw material

A technology of succinic acid and straw, which is applied in the field of bioengineering to achieve environmental friendliness, reduce the cost of medium, and solve the problem of utilization

Inactive Publication Date: 2008-07-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Effect test

Embodiment 1

[0039] Seed medium (per L): glucose 5g, yeast extract 5g, K 2 HPO 4 ·3H 2 O 1.0g, NaH 2 PO 4 2H 2 O 1.0g, mixed vitamins 1mL, pH 7.0. Anaerobic culture at 37°C for 24h.

[0040] Fermentation medium (per L): Enzymatically hydrolyzed straw syrup (total reducing sugar) 45g, yeast extract 15g, corn steep liquor 20g, MgCl 2 0.2g, Na 2 HPO 4 12H 2 O 1.5g, NaH 2 PO 4 2H 2 O 1.5g, pH 6.5. Anaerobic culture at 37°C for 48h.

[0041] Enzymatically hydrolyzed straw syrup is prepared using the general method reported in the literature as described in the summary of the invention above, which is briefly described as follows:

[0042] (1) Dilute alkali treatment-enzyme hydrolysis: roughly cut plant fiber raw materials such as corn stalks to a length of 20-30mm, soak in 1% NaOH dilute lye at 85-90°C for 1 hour, filter to remove the black liquor residue, and filter Fiber hemifiber; then use cellulolytic enzyme (10-20FPU / g total sugar), enzymatic hydrolysis for 48h, filter to obt...

Embodiment 2

[0051] According to the method of Example 1, different plant stalks (corn stalks, corncobs, rice straw, wheat straw) were treated with dilute alkali treatment-enzymolysis, and batch fermentation was carried out in anaerobic bottle fermentation. The initial reducing sugar concentration was 45g / L. Table 2 shows the results of succinic acid production from hydrolyzed syrup of various plant straw raw materials.

[0052] Table 2 The results of succinic acid fermentation of different plant straw enzymatically hydrolyzed syrup (anaerobic bottle batch fermentation)

[0053]

[0054] It can be seen from the above results of succinic acid production by fermentation of several different plant straw hydrolyzed syrups that the Actinobacillus succinogenes CGMCC 1593 screened by the present invention is suitable for fermenting straw hydrolyzed syrups from different plant sources. Among them, the fermentative production of succinic acid by enzymatic hydrolysis of corn stalk syrup is...

Embodiment 3

[0056] According to the method of Example 1, using dilute alkali pretreatment-enzymolysis corn stalk hydrolyzed syrup, fermented in batches with anaerobic bottles, when the initial reducing sugar concentration is 30, 45, 55, 65, 85 g / L respectively, the results are shown in the table 3 shows:

[0057] Table 3 Results of succinic acid fermentation of corn stover syrup with different initial reducing sugar concentrations (anaerobic bottle batch fermentation)

[0058] initial reducing sugar

[0059] As can be seen from Table 3, when the initial reducing sugar concentration is 45g / L, the yield of succinic acid is the highest, reaching 33.7g / L, the yield of succinic acid to consumed sugar is 81.0%, and the utilization rate of sugar is 92.6%.

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Abstract

A method for using straw raw materials to ferment and produce succinic acid belongs to the technical field of biological engineering. The invention takes straws as raw materials and obtains hydrolysis straw syrup of hexose and pentose through hydrolysis treatment, and then succinic acid actinobacillus CGMCC 1593 is used to ferment and produce the succinic acid in culture medium of the hydrolysis straw syrup which contains reducing sugar. The microorganism CGMCC 1593 of the invention which is obtained through screening from paunches is fed-batched and anaerobically fermentted for 48 hours in a 5L-25L fermenter under the anaerobical circumstance whose pH is kept within 5.5-7.5. The concentration of total reducing sugar which is invested is 68.6-70.9g/L, the concentration of the succinic acid which is produced is 49.5-52.1g/L, the yield of the succinic acid to consumption suger is 80.0%-81.2%( the availability ratio of the sugar is 87.3-93.6%), and the production intensity is 1.03-1.09g/(L h). The invention has the prominent advantages that the raw materials of agricultural wastes straws are utilized to ferment and produce the succinic acid, the method is a producing method which uses renewable raw materials and is environmental friendly, and the problem of petrifaction resources tension of chemosynthesis succinic acid can be alleviated.

Description

technical field [0001] The invention relates to a method for producing succinic acid by fermenting straw raw materials, belonging to the technical field of bioengineering. Background technique [0002] Succinic acid, also known as succinic acid, has the molecular formula C 4 h 6 o 4 , with a molecular weight of 118.09, is a common natural organic acid that widely exists in the human body, animals, plants and microorganisms. [0003] Succinic acid is an important four-carbon compound in industry. It is widely used in food, medicine, pesticides, dyes, spices, paints, plastics and material industries as organic synthesis raw materials, intermediate products or professional chemicals. Its largest market is used in surfactants, detergents, green solvents, ion chelating agents, biodegradable plastics and other fields. It is used in the food industry as an acidulant, pH modifier, flavoring substance and antibacterial agent, and as a raw material or intermediate for the producti...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12N1/20C12R1/04
Inventor 孙志浩郑璞倪晔董晋军
Owner JIANGNAN UNIV
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