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Broad spectrum influenza virus resistant antagonistic peptide nasal spray preparation and application

An influenza virus and antagonism technology, applied in the directions of antiviral agents, aerosol delivery, peptides, etc., can solve the problems of long production time, shortage of raw material supply, high price, small toxic side effects, easy mass production, and high curative effect. Effect

Inactive Publication Date: 2008-08-13
北京金迪克生物技术有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, even if the production capacity is increased on a large scale, its production is greatly restricted due to the shortage of raw material supply.
[0008] Moreover, when it is widely used in the population, the influenza virus can produce a certain degree of drug resistance to this drug, coupled with the high price and long production time, which cannot meet the needs of preventing influenza / bird flu pandemics, so new technology is used to develop New anti-influenza virus drugs with NA inhibitory activity are urgently needed and necessary

Method used

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  • Broad spectrum influenza virus resistant antagonistic peptide nasal spray preparation and application
  • Broad spectrum influenza virus resistant antagonistic peptide nasal spray preparation and application
  • Broad spectrum influenza virus resistant antagonistic peptide nasal spray preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] 1. Preparation of influenza virus neuraminidase inhibitory polypeptide

[0091] a. According to the operating guidelines for peptide library screening, take A / PR / 8 / 34 (H1N1) influenza virus as the target molecule, and screen influenza virus antagonistic polypeptides from the phage random 9-peptide library;

[0092] b. Identify the neuraminidase inhibitory activity of the screened polypeptide through ELISA and in vitro NA activity inhibition experiments;

[0093] c. Sequencing the identified polypeptide and synthesizing a consensus sequence, the consensus sequence being WPKLHVHPR or SWIHHFRPH.

[0094] 2. Preparation of broad-spectrum anti-influenza virus antagonist peptide nasal spray

[0095] Put the above-prepared influenza virus neuraminidase inhibitory polypeptide, natural polysaccharide chitosan, and cytokine human interferon into a 100ml volumetric flask, and add physiological saline to the mark. Wherein, the concentration of influenza virus neuraminidase inhibi...

Embodiment 2

[0097] 1. Preparation of influenza virus neuraminidase inhibitory polypeptide

[0098] a. According to the operating guidelines for peptide library screening, take A / FM / 1 / 47 (H1N1) influenza virus as the target molecule, and screen influenza virus antagonistic polypeptides from the phage random 10 peptide library;

[0099] b. Identify the neuraminidase inhibitory activity of the screened polypeptide through ELISA and in vitro NA activity inhibition experiments;

[0100] c. Sequencing the identified polypeptides and synthesizing a consensus sequence, the consensus sequence being HWHPHSHIQP or PLQASLHHIS.

[0101] 2. Preparation of broad-spectrum anti-influenza virus antagonist peptide nasal spray

[0102] Put the above-prepared influenza virus neuraminidase inhibitory polypeptide, natural polysaccharide starch, cytokines human interferon, and human interleukin-2 into a 100ml volumetric flask, and add physiological saline to the mark. Among them, the concentration of influenza...

Embodiment 3

[0104] 1. Preparation of influenza virus neuraminidase inhibitory polypeptide

[0105] a. According to the operating guidelines for peptide library screening, take A / Sydney / 5 / 97 (H3N2) influenza virus as the target molecule, and screen influenza virus antagonistic polypeptides from the phage random 11 peptide library;

[0106] b. Identify the neuraminidase inhibitory activity of the screened polypeptide through ELISA and in vitro NA activity inhibition experiments;

[0107] c. Sequencing the identified polypeptide and synthesizing a consensus sequence, the consensus sequence is HKHNGKAWPLS or LNILSTLYKWR.

[0108] 2. Preparation of broad-spectrum anti-influenza virus antagonist peptide nasal spray

[0109] Place the above-prepared influenza virus neuraminidase inhibitory polypeptide, natural polysaccharide starch and chitosan, human interferon, human interleukin-2, and human interleukin-12 in a 100ml volumetric flask, add normal saline to scale. Among them, the concentratio...

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Abstract

The invention pertains to application field of biological technology, discloses a broad-spectrum anti-flue nasal cavity sprayer with antagonistic peptide, which can prevent and cure influenza virus, including infection of bird-flue. Advantages of the medicament is special recipe and usage thereof. The nasal cavity sprayer mainly comprises: influenza virus NA inhibiting short peptide as main active ingredient of the medicament, which can inhibit reproduction of influenza virus; cytokines of multiple types and anti-virus peptides with obvious cooperation therebetween, which can strengthen activity of anti-flue peptide. Natural polysaccharide, which is Muco-adhesive and stabilizer, can increase residence time of the medicament on nasal epithelium, and can enhance activity of interferon. The invention utilize unique and quantitative nasal cavity sprayer delivery device, with which the patient can deliver medicament directly into nasal cavity, so that the operation is easy and painless.

Description

technical field [0001] The invention relates to the development of a broad-spectrum anti-influenza virus antagonistic peptide nasal cavity spray preparation, which is applied to the prevention and treatment of human influenza virus infection and belongs to the field of pharmaceutical preparations. Background technique [0002] Influenza (abbreviated as influenza) is an acute respiratory infectious disease caused by influenza virus, which is highly contagious. The epidemiological characteristics of influenza are: sudden outbreak, rapid spread, and wide spread. Annual seasonal influenza epidemics and periodic influenza pandemics seriously endanger human health. The annual incidence of influenza in my country is 10%-30%. Influenza causes about 500 million people to become sick and 250,000 to 500,000 people to die every year in the world, causing huge economic losses and disease burden. WHO believes that if a new influenza pandemic occurs, 2-7 million people will die globally....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K7/06C07K7/08A61K38/08A61K38/10A61K38/16A61K9/12A61K47/36A61P31/12
Inventor 侯云德匡治州郑丽舒
Owner 北京金迪克生物技术有限责任公司