Method for producing food and rink fag-end solid-state fermentation protein feed stuff, composite zymocyte liquid and preparing method thereof
A technology for compound fermentation bacteria and food waste residues, applied in animal feed, animal feed, applications, etc., can solve problems such as the inability to meet the requirements of environment, hygiene and health, the increase of leachate and biogas production, and the reduction of the average calorific value of garbage. , to achieve the effect of improving the effective utilization rate, high soluble protein content, and alleviating the contradiction between humans and animals competing for food
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Embodiment 1
[0031] A method for producing high-protein feed by utilizing multi-strain joint solid-state fermentation of catering waste, comprising the following steps:
[0032] 1. Preparation of composite bacterial solution
[0033] (1) Select the following microbial strains as the bacterial source: Geotrichum candidum, Yarrowia lipolytica, Phanerochaete chrysosporium.
[0034] (2) The preparation process of the composite bacterial solution is as follows:
[0035] Slope culture → liquid strain → liquid primary seed (shake flask) cultivation → liquid secondary seed cultivation → determination of bacterial count → mixing of bacterial strains in proportion → fermentation liquid
[0036] Concrete preparation process is as follows:
[0037] 1) Slant strain culture: Geotrichum candidum and Yarrowia lipolytica slant culture adopts a culture medium containing 0.8% peptone, 1.0% glucose, 0.3% beef extract, 2.0% agar, and the rest is distilled water at pH 7.2 to 7.4; Phanerochaete slant culture ...
Embodiment 2
[0044]1. The preparation of catering waste residue fermentation bacteria liquid and the treatment of catering waste residues are the same as in Example 1. The number of live bacteria in each fermentation bacteria liquid is measured. : 0.6: 0.6 were mixed to make a compound fermentation broth.
[0045] 2. Preliminary screening of catering waste residue, picking out non-fermentable materials such as plastics, crushing and mixing the bone, and measuring the initial waste residue composition. The pH value is about 6.4, the salt content is 1% to 2%, and the oil content is about 10%. The crude fiber content is 8% to 15%, the crude protein content is 6% to 12%, and the soluble protein content is about 1.09mg / g; adjust the pH value between 6 and 7, and the moisture content between 65% and 70%. Add mixed strains at 30°C, the inoculation ratio is 15%-20%, solid-state fermentation for 78-84 hours, dry and sterilize, and it can be made into pH value 6-7, salt content 1%-2%, oil content 2...
Embodiment 3
[0047] 1. The preparation of catering waste residue fermentation bacteria liquid and the treatment of catering waste residues are the same as in Example 1. The number of live bacteria in each fermentation bacteria liquid is measured. : 1: 0.6 for mixing to make a compound fermentation broth.
[0048] 2. Preliminary screening of catering waste residue, picking out non-fermentable materials such as plastics, crushing and mixing the bone, and measuring the initial waste residue composition. The pH value is about 6.4, the salt content is 1% to 2%, and the oil content is about 10%. The crude fiber content is 8% to 15%, the crude protein content is 6% to 12%, and the soluble protein content is about 1.09mg / g; adjust the pH value between 6 and 7, and the moisture content between 65% and 70%. Add mixed strains at 30°C, the inoculation ratio is 15%-20%, solid-state fermentation for 78-84 hours, dry and sterilize, and it can be made into pH value 6-7, salt content 1%-2%, oil content 2%...
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