Method for preparing nano-multiple phase calcium phosphate/fibroin protein composite bracket

A complex calcium phosphate and composite scaffold technology is applied in the field of nano-BCP/SF composite scaffolds, which can solve the problems of low material strength, unable to meet the needs of bearing bone defect repair, damage to the biological activity of materials, and poor stomatal connectivity.

Inactive Publication Date: 2008-10-29
JIAMUSI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing methods for preparing scaffolds, either the introduction of toxic organic solvents damages the biological activity of the material, or the internal structure of the material has small pore size, low porosity, and poor pore connectivity, which is not conducive to the adhesion, differentiation, and formation of bone cells. Proliferation and growth; the strength of the prepared material is too low to meet the needs of bearing bone defect repair.

Method used

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Examples

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Embodiment approach 1

[0007] Concrete embodiment one: the operation steps of this embodiment are: a. use calcium chloride and diammonium hydrogen phosphate as raw materials for generating calcium-deficient hydroxyapatite, the concentration of the reaction solution is 0.2M, and the particle size is 90-300 μm Silk fibroin powder is added as a template in calcium chloride solution to regulate the morphology of HA. According to the mass ratio, the mass ratio of HA to SF is (7-3): (3-7), reacted at different temperatures, ammonia water and hydrochloric acid adjusted The pH value of the reaction solution is 7-10, react for 2-3 hours, age for 20 hours, and then centrifuge and dry to obtain HA / SF composite powder; b. Compress the HA / SF composite powder and sinter it in an air furnace at atmospheric pressure. The sintering temperature is 900-1100°C, and the holding time is 2-3 hours to obtain a porous nano-composite calcium phosphate ceramic matrix; c. impregnate the porous nano-composite calcium phosphate c...

Embodiment approach 2

[0008] Concrete embodiment two: the operation steps of this embodiment are: a. use calcium chloride and diammonium hydrogen phosphate as raw materials for generating hydroxyapatite, add distilled water to prepare a reaction solution with a concentration of 0.2M and a particle size of 90 to 300 μm The silk fibroin powder is added as a template in the calcium chloride solution to regulate the morphology of HA. According to the mass ratio, the mass ratio of HA and SF is 7:3, react at room temperature, adjust the pH value of the reaction solution with ammonia water and hydrochloric acid to 10, and react 2 hours, after aging for 20 hours, centrifuge and dry to get HA / SF composite powder b. Press 2g of HA / SF composite powder under 8MPa pressure to make The green body of 15mm×5mm is sintered in an air furnace at atmospheric pressure, the sintering temperature is 900°C, and the holding time is 3 hours to obtain a porous nano-composite calcium phosphate ceramic matrix; the porosity is ...

Embodiment approach 3

[0009] Concrete embodiment three: the operation steps of this embodiment are: a. use calcium chloride and diammonium hydrogen phosphate as raw materials for generating hydroxyapatite, add distilled water to prepare a reaction solution with a concentration of 0.2M and a particle size of 90 to 300 μm The silk fibroin powder was added as a template to the calcium chloride solution to regulate the morphology of HA. According to the mass ratio, the mass ratio of HA and SF was 5:5, and reacted at 40°C. The pH value of the reaction solution was adjusted by ammonia water and hydrochloric acid to 7, and the reaction 3 hours, after aging for 20 hours, centrifuge and dry to get HA / SF composite powder b. Press 2g of HA / SF composite powder under 8MPa pressure to make The green body of 15mm×5mm is sintered in an air furnace at atmospheric pressure, the sintering temperature is 1000°C, and the holding time is 3 hours to obtain a porous multi-phase calcium phosphate ceramic matrix; the porosi...

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Abstract

A preparation method of nanometer multiphase calcium phosphate / fibroin protein composite bracket relates to a preparation method of tissue engineering cytoskeleton. The existing preparation technology has the defects that organic solvent goes against the introduction of active material, sealed stomata goes against the growth of osseous tissue, low mechanical strength is unsuitable for bearing bone reparative regeneration and tissue distribution is uneven. The method provided by the present invention includes: a. calcium-deficient nanometer hydroxyapatite powder is synthesized by the effect offibroin organic template; b. pore-foaming agent sintering technique is adopted to crack the calcium-deficient nanometer hydroxyapatite powder after press molding for further preparation of nano-porous multiphase calcium phosphate ceramic matrix, the sintering temperature is 900 to 1,100 DEG C, the time is 2 to 3 hours and the pore-foaming agent is fibroin powder with the gain diameter of 90 to 300Mum; c. the matrix is dipped in 1 to 5 percent fibroin solution, frozen and dried to obtain the nanometer multiphase calcium phosphate / fibroin protein composite bracket material. The low-temperature sintering of the method provided by the present invention maintains the biological activity of the ceramics and overcomes the defects of the existing technology.

Description

Technical field: [0001] The present invention relates to a preparation method of tissue engineering porous composite cell scaffold, in particular to a kind of calcium-deficient nano-hydroxyapatite (HA) pyrolysis to prepare nano-composite calcium phosphate (BCP) ceramic matrix with uniform distribution of macroscopic porous tissue, and then impregnated A method for preparing nano-BCP / SF composite scaffolds with high specific surface area by freeze-drying silk fibroin (SF) solution. Background technique: [0002] Porous HA can induce the growth of surrounding bone tissue and form a firm chemical bond with the bone. It has excellent osteoconductivity, can be better integrated into the host bone, has a strong affinity for bone morphogenetic proteins, and is non-toxic Side effects and no carcinogenic effect, so it is widely used as a physiological scaffold for hard tissue repair materials, but the extremely low degradation rate cannot make it used as a degradable material, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/42
Inventor 李慕勤王静孟祥才
Owner JIAMUSI UNIVERSITY
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