Method for preparing nano-multiple phase calcium phosphate/fibroin protein composite bracket

Abstract

A preparation method of nanometer multiphase calcium phosphate / fibroin protein composite bracket relates to a preparation method of tissue engineering cytoskeleton. The existing preparation technology has the defects that organic solvent goes against the introduction of active material, sealed stomata goes against the growth of osseous tissue, low mechanical strength is unsuitable for bearing bone reparative regeneration and tissue distribution is uneven. The method provided by the present invention includes: a. calcium-deficient nanometer hydroxyapatite powder is synthesized by the effect offibroin organic template; b. pore-foaming agent sintering technique is adopted to crack the calcium-deficient nanometer hydroxyapatite powder after press molding for further preparation of nano-porous multiphase calcium phosphate ceramic matrix, the sintering temperature is 900 to 1,100 DEG C, the time is 2 to 3 hours and the pore-foaming agent is fibroin powder with the gain diameter of 90 to 300Mum; c. the matrix is dipped in 1 to 5 percent fibroin solution, frozen and dried to obtain the nanometer multiphase calcium phosphate / fibroin protein composite bracket material. The low-temperature sintering of the method provided by the present invention maintains the biological activity of the ceramics and overcomes the defects of the existing technology.

Description

Technical field:

[0001] The present invention relates to a preparation method of tissue engineering porous composite cell scaffold, in particular to a kind of calcium-deficient nano-hydroxyapatite (HA) pyrolysis to prepare nano-composite calcium phosphate (BCP) ceramic matrix with uniform distribution of macroscopic porous tissue, and then impregnated A method for preparing nano-BCP / SF composite scaffolds with high specific surface area by freeze-drying silk fibroin (SF) solution. Background technique:

[0002] Porous HA can induce the growth of surrounding bone tissue and form a firm chemical bond with the bone. It has excellent osteoconductivity, can be better integrated into the host bone, has a strong affinity for bone morphogenetic proteins, and is non-toxic Side effects and no carcinogenic effect, so it is widely used as a physiological scaffold for hard tissue repair materials, but the extremely low degradation rate cannot make it used as a degradable material, which ...