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Method for preparing poly-N-2-carboxyethyl pyrrole metallic chelate nano-tube used as affinity adsorbing medium

A technology of carboxyethylpyrrole and carboxyethylpyrrole, which is applied in the field of affinity adsorption medium materials, can solve the problems of strong non-specific adsorption, unsatisfactory purification effect, and pressure resistance, and achieve small non-specific adsorption and good chemistry. Stability, effect of increasing adsorption capacity

Inactive Publication Date: 2008-10-29
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this type of medium is that it is soft, not pressure-resistant, and easy to degrade in a lower pH range.
Porous glass with controlled pore size is a unique type of medium in AC, which is insoluble and hardly affected by changes in eluent, pressure, flow rate, pH and ionic strength; but the non-specific adsorption is strong, and the purification effect is not ideal
However, there are no related reports on the use of nanotubes as affinity adsorption media.

Method used

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  • Method for preparing poly-N-2-carboxyethyl pyrrole metallic chelate nano-tube used as affinity adsorbing medium
  • Method for preparing poly-N-2-carboxyethyl pyrrole metallic chelate nano-tube used as affinity adsorbing medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Preprocessing of templates:

[0025] Take a polycarbonate membrane with a pore size of 0.8 μm, a thickness of 20 μm, and a diameter of 25 mm, and place it in a vacuum-tight device. Add 6 mL of 0.432M N-2-carboxyethylpyrrole aqueous solution, and use a vacuum pump to The vacuum of the device is maintained at 30 Pa for 30 minutes.

[0026] 2) Oxidation deposition of poly N-2-carboxyethylpyrrole:

[0027] Take out step 1) and fix the processed template in the middle of the diffusion cell device, and then add 6 mL of 1.728M ferric chloride solution and 6 mL of 0.432M N-2-carboxyethylpyrrole aqueous solution into the two cells respectively. React at 100°C for 5h.

[0028] 3) Formation of poly N-2-carboxyethylpyrrole nanotubes:

[0029] After the reaction reaches the set time, pour out the solution in the two tanks, take out the template after step 2), immediately rinse the surface with distilled water, leave it to dry at room temperature, and use alumina to polymerize the sur...

Embodiment 2

[0035] 1) Preprocessing of templates:

[0036] Take a polycarbonate membrane with a pore size of 0.2 μm, a thickness of 20 μm, and a diameter of 25 mm, and immerse it in a 6 mL N-2-carboxyethylpyrrole aqueous solution with a concentration of 0.144 M for 2 hours.

[0037] 2) Oxidation deposition of poly N-2-carboxyethylpyrrole:

[0038] Take out the template processed in step 1 and fix it in the middle of the diffusion cell device, then add 6 mL of 0.576M ferric chloride solution and 6 mL of 0.144M N-2-carboxyethylpyrrole aqueous solution into the two cells respectively, and react at 60℃ 4h.

[0039] 3) Formation of poly N-2-carboxyethylpyrrole nanotubes:

[0040] The desired poly-N-2-carboxyethylpyrrole nanotubes were prepared according to the method in step 3 of Example 1.

[0041] 4) Activated nanotube coupling ligand:

[0042] The nanotubes were immersed in 5 mL of a mixed aqueous solution of HOAt and EDC for activation, the concentration of EDC was 0.368 mg / L, the concentration ...

Embodiment 3

[0046] 1) Preprocessing of templates:

[0047] Take a polycarbonate membrane with a pore size of 0.4 μm, a thickness of 20 μm, and a diameter of 25 mm, and immerse it in an aqueous solution of N-2-carboxyethylpyrrole with a volume of 6 mL and a concentration of 0.144 M for 2 hours.

[0048] 2) Oxidation deposition of poly N-2-carboxyethylpyrrole:

[0049] Take out the template processed in step 1 and fix it in the middle of the diffusion cell device, then add 6 mL of 0.72M ferric chloride solution and 6 mL of 0.288M N-2-carboxyethylpyrrole aqueous solution into the two cells respectively. React at 70°C for 5h.

[0050] 3) Formation of poly N-2-carboxyethylpyrrole nanotubes:

[0051] The desired poly-N-2-carboxyethylpyrrole nanotubes were prepared according to the method in step 3 of Example 1.

[0052] 4) Activated nanotube coupling ligand:

[0053] The nanotubes were immersed in 5 mL of a mixed aqueous solution of HOAt and EDC for activation, the concentration of EDC was 0.552 mg...

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Abstract

A preparation method of poly N-2-carboxyethyl pyrrole metal chelating nanometer pipe used as affinity absorbing medium relates to an affinity absorbing medium material. The present invention provides a preparation method of poly N-2-carboxyethyl pyrrole metal chelating nanometer pipe used as affinity absorbing medium. Polycarbonate membrane is taken as the template to aggregate N-2-carboxyethyl pyrrole monomer in the membrane hole; then the template is removed to obtain the poly N-2-carboxyethyl pyrrole nanometer pipe. The nanometer pipe is activated by 1-(3-dimethylin propyl)-3-ethyl carbodiimide and 1-hydroxide radical-7-azobenzene benzotriazole, coupled with iminodiacetic acid, and chelated with dentate Cu2+ on the internal surface and the external surface of the nanometer pipe, thus obtaining the poly N-2-carboxyethyl pyrrole metal chelating nanometer pipe. The prepared poly N-2- carboxyethyl pyrrole metal chelating nanometer pipe provided with a large number of affinity absorption loci has the advantages of large affinity capacity, small non-specific absorption, good chemical stability, good hydrophilicity and good biocompatibility, which can be used for protein separation and purification.

Description

Technical field [0001] The invention relates to an affinity adsorption medium material, in particular to a preparation method of poly N-2-carboxyethylpyrrole metal chelate nanotubes used as an affinity adsorption medium. Background technique [0002] In the fields of bioengineering and life sciences, there are many protein products with high application value and economic value. The separation and purification of these products is very important in the production process. Affinity chromatography (AC) has become one of the most effective techniques for protein purification due to its high selectivity, high yield, and the technical advantages of obtaining high-purity products in one step. AC is based on the ability of ligands to bind specifically and reversibly to target products such as proteins through van der Waals forces, hydrophobic forces, electrostatic forces, etc., to separate the desired target products from complex biological samples. The AC medium plays a pivotal role in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/30C07K1/22
Inventor 石巍曹慧慧葛东涛张其清
Owner XIAMEN UNIV