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Hydrogel material for repairing central nervous and preparation thereof

A central nervous system and hydrogel technology, which is applied in the field of hydrogel materials for central nervous system repair, can solve problems such as unsatisfactory repair effects, and achieve good magnetic field loading characteristics, no magnetic field attenuation, and good biocompatibility

Inactive Publication Date: 2008-11-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional brain defect repair materials use polyacrylamide hydrogel, polycarbonate, water-soluble gel polyethylene glycol, N-(2-hydroxypropyl) methacrylamide (HPMA), etc. It promotes the regeneration of axons. In addition, it is reported that self-made biological scaffolds formed by lysine, alanine, and aspartic acid can have the characteristics of promoting the adhesion, differentiation and extensive growth of various types of nerve cells. And can promote the formation of synapses between neurons, but the repair effect of these materials is not ideal enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: polyethylene glycol nano iron oxide-D type polylysine hydrogel

[0017] Glutaraldehyde is used to cross-link the mixed solution of polyethylene glycol nanometer iron oxide and D-type polylysine, and the cross-linked product is freeze-dried to form a film. Concrete preparation steps are as follows:

[0018] (1) Reagent preparation

[0019] a solution: polyethylene glycol was dissolved in distilled water at 25°C to obtain a 1 wt% polyethylene glycol solution.

[0020] Solution b: D-type polylysine (molecular weight: 15000 Da) wrapped nano-iron oxide was dispersed in distilled water at 25°C, the final concentration of iron element was 4.2 μg / ml, and the final concentration of D-type polylysine was 0.5 wt%. Mix solution a and solution b and shake for 30 minutes on a non-iron tray shaker.

[0021] (2) Cross-linking: add glutaraldehyde solution, the final concentration of glutaraldehyde solution is 1 wt%, react at 4°C for 4 hours.

[0022] (3) The final slu...

Embodiment 2

[0025] Embodiment 2: polyethylene glycol nano iron oxide-staphylococcal protein A hydrogel

[0026] Glutaraldehyde is used to cross-link the mixed solution of polyethylene glycol nano-iron oxide and staphylococcal protein A, and the cross-linked product is freeze-dried to form a film. Concrete preparation steps are as follows:

[0027] (1) Reagent preparation

[0028] a solution: polyethylene glycol was dissolved in distilled water at 25° C. to obtain a 2 wt % polyethylene glycol solution.

[0029] Solution b: Disperse staphylococcal protein A-coated nano-iron oxide in distilled water at 25°C, the final concentration of iron element is 84 μg / ml, the final concentration of staphylococcal protein A is 1wt%, mix a solution and b solution, and place Shake for 30 min on a non-iron tray shaker.

[0030] (2) Cross-linking: add glutaraldehyde solution, the final concentration of glutaraldehyde solution is 2wt%, react at 4°C for 2 hours.

[0031] (3) The final slurry was poured int...

Embodiment 3

[0034] Embodiment 3: polyethylene glycol nano iron oxide-staphylococcal protein A-nerve growth factor (NGF) hydrogel

[0035] Glutaraldehyde was used to cross-link the mixed solution of polyethylene glycol nano-iron oxide and staphylococcal protein A-nerve growth factor (NGF), and the cross-linked product was freeze-dried to form a film. Concrete preparation steps are as follows:

[0036] (1) Reagent preparation

[0037] a solution: polyethylene glycol was dissolved in distilled water at 25° C. to obtain a 5 wt % polyethylene glycol solution.

[0038] Solution b: Mix 1 ml of physiological saline containing 100 ng NGF and 10 ml of staphylococcal protein A.

[0039] Solution c: Disperse solution b coated with nano-iron oxide in distilled water at 25° C.; the final concentration of iron element is 21 μg / ml, and the final concentration of staphylococcal protein A is 2 wt%. Mix solution a and solution c and shake for 2 hours on a non-iron tray shaker.

[0040] (2) Cross-linking...

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Abstract

The invention discloses a hydrogel material for nervus centralis repair and a method for making the same. The method comprises the following steps of: mixing polyethylene glycol water solution with density of 1 to 5 weight percent with polylysine or staphylococcal protein A and nanometer ferric oxide water solution with the density of 0.1 to 2 weight percent, oscillating and stirring; adding glutaral pentanedial solution until reaching final density of 1 to 4 weight percent of the glutaral pentanedial, and reacting for 2 to 24 hours below 4 DEG C; freezing and drying; and cleaning as well as renewly freezing and drying. The hydrogel material for nervus centralis repair made in the method has the advantages of good biocompatibility, rheological characteristics and mechanical properties similar to the nervus centralis tissue, no secondary damage to tissue after implantation of the nervus centralis, and is capable of regulating biodegradability in a large scale and is applicable to nervus repair. And the making method has simple process, low cost, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a central nervous tissue engineering framework material, in particular to a hydrogel material for central nervous repair. Background technique [0002] Tissue engineering scaffold materials play the following roles in nerve regeneration: inhibiting the formation of glial scars: providing bridges for the growth of blood vessels and nerve axons; serving as carriers of growth factors and replacing cells. Traditional brain defect repair materials use polyacrylamide hydrogel, polycarbonate, water-soluble gel polyethylene glycol, N-(2-hydroxypropyl) methacrylamide (HPMA), etc. It promotes the regeneration of axons. In addition, it is reported that self-made biological scaffolds formed by lysine, alanine, and aspartic acid can have the characteristics of promoting the adhesion, differentiation and extensive growth of various types of nerve cells. And it can promote the formation of synapses between neuron cells, but the repair effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/06A61L31/16A61L31/14
Inventor 邵阳杨期东肖波
Owner CENT SOUTH UNIV
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