Microorganism decomposition agent and method for preparing same

A technology for microorganisms and preservatives, applied in the field of microbial preservatives and their preparation, can solve the problems of poor stability of genetic traits and unstable product effects, and achieve a wide range of applications, strong resistance to stress and adaptability and decomposition or degradation. Ability, the effect of a small amount of use

Inactive Publication Date: 2008-11-26
成都合成生物科技有限公司
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  • Abstract
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Problems solved by technology

And the inoculants are all amplified bacteria. Because the amplified bacteria have been propagated for severa

Method used

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  • Microorganism decomposition agent and method for preparing same
  • Microorganism decomposition agent and method for preparing same
  • Microorganism decomposition agent and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048]The preparation method of the microbial decay agent comprises three steps of seed cultivation, industrial seed preparation, and mixing and drying.

[0049] (1) Seed cultivation:

[0050] Yeast culture:

[0051] Saccharomyces cerevisiae, Coccidioides immobilis, Heterotype Hansen, and Saccharomyces cerevisiae were inoculated into 2L culture bottles (0.4kg wort potato sucrose medium was contained in the bottle), and each Three of each were cultured for 36 hours at a culture temperature of 36-42° C. and a shaker rotation speed of 200 rpm; the wort potato sucrose medium was a conventional medium, and its dosage was a conventional dosage.

[0052] Cellulolytic Bacteria Culture:

[0053] The cellulolytic bacteria on the slant medium of each purification culture: Bacillus licheniformis, Pseudomonas bacterium, bright filament bacteria, Alcaligenes, Bacillus subtilis pure species were inoculated into 2L culture bottles (0.4kg beef in the bottle) respectively. Extract peptone cu...

Embodiment 2

[0066] After seed cultivation, 1.00kg of Saccharomyces cerevisiae, 1.12kg of Coccidioides immobilis, 1.40kg of Heterotype Hansenula, and 1.20kg of Saccharomyces cerevisiae were mixed evenly, and inoculated into a 200L fermenter (140kg of wort potato sucrose culture solution) for cultivation, stirring The rotation speed is 220rpm, the culture temperature is 45-55°C, and the culture time is 86 hours;

[0067] cellulolytic bacteria

[0068] After seed cultivation, 1.20kg of Bacillus licheniformis, 1.36kg of Pseudomonas, 1.28kg of Bacillus lucidum, 1.04kg of Bacillus alcaligenes, and 1.32kg of Bacillus subtilis were mixed evenly, and inoculated into a 200L fermenter (fitted with beef extract peptone culture) base+bamboo yellow paper 140kg), the stirring speed is 220rpm, the cultivation temperature is 50-60°C, and the cultivation time is 48 hours.

[0069] Clean the fresh vegetables, change them into small pieces, place them in a fermenter, inoculate 1.2kg of Lactobacillus delnabr...

Embodiment 3

[0072] After seed cultivation, 0.80 kg of Saccharomyces cerevisiae, 1.00 kg of Coccidioides immobilis, 1.50 kg of Heterotype Hansenula, and 1.50 kg of Saccharomyces cerevisiae were mixed evenly, and inoculated into a 200L fermenter (140kg of wort potato sucrose culture solution) for cultivation, and stirred The rotation speed is 200rpm, the culture temperature is 45-55°C, and the culture time is 72 hours;

[0073] After seed cultivation, 1.20kg of Bacillus licheniformis, 1.60kg of Pseudomonas, 1.30kg of Bacillus lucidum, 0.70kg of Bacillus alcaligenes, and 1.20kg of Bacillus subtilis were mixed evenly, and inoculated into a 200L fermenter (fitted with beef extract peptone culture) base+bamboo yellow paper 140kg), the stirring speed is 180rpm, the cultivation temperature is 50-60°C, and the cultivation time is 48 hours.

[0074] The industrial original seed cultivation of Lactobacillus is the same as in Example 1.

[0075] After the fermentation and cultivation of each bacteri...

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Abstract

The invention discloses a microbe decomposition agent and a preparation method thereof. The microbe decomposition agent of the invention is prepared according to soil humic acid and humification factors and formed by optimum combination of different physiologic races and species group of yeast, cellulolytic bacteria and lactobacillus. The preparation method for the invention consists of three steps: seed culture; industrial making of original seed; blending every component according to the weight percentages and drying. The microbe decomposition agent of the invention has the advantages of stronger stress resistance, adaptability, decomposition or degradation capability, smaller amount, convenience in use, wider application scope, short decomposition cycle and good effect. Under the premise of guaranteeing the existing farming system not to be destroyed, the agent can not only improve comprehensive productivity of soil by returning agricultural residues to the fields for soil fertility and productivity but also effectively avoid environment pollution caused by the fact that farmers burn a large amount of agriculture residues owing to seasonal contradiction of crops for rotation.

Description

technical field [0001] The present invention relates to a microbial scavenging agent and a preparation method thereof, in particular to a septic stimulant composed of various microbial protobacteria and a preparation method thereof. Background technique [0002] The long-term high-load open agricultural ecosystem characterized by high multiple cropping index makes the only resource of agriculture, the cultivated land, overwhelmed, and has brought a relatively large negative impact on agriculture. On the one hand, a large amount of straw garbage produced in agricultural planting areas with a high multiple cropping index has become a source of land, water, and air pollution; The soil humus, fertilizer, gas, and heat decrease year by year, the number of microorganisms in the soil decreases, the organic matter content becomes less and less, the soil becomes compacted and acidified, and the comprehensive fertility of cultivated land decreases year by year. However, soil compacti...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/20C05F11/08
Inventor 段儒斌
Owner 成都合成生物科技有限公司
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