Acellular dermal matrix compound film material and preparation method thereof

A technology of decellularized dermis and decellularized porcine dermis, applied in medical science, prostheses, etc., can solve problems such as poor mechanical properties and formability, limited pore size and porosity, and limited applications, so as to enhance interface binding ability , Improve the physical and mechanical properties, the effect of performance improvement

Inactive Publication Date: 2008-12-03
JIANGYIN BENXIANG BIOTECHOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. The acellular dermal matrix has the advantages of excellent mechanical properties and excellent formability, but at the same time, the obvious shortcomings such as limited pore size and porosity and poor adhesion limit its application as a biomedical material;
[0009] 2. Collagen basement membrane material has obvious advantages such as controllable pore size, porosity structure and degradation performance, the potential to obtain various excellent properties through the compounding of various materials, good adhesion ability and cell proliferation environment, etc., but its Poor mechanical properties and formability largely limit its application in the biomedical field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Preparation of collagen solution

[0043] Take 10 parts by weight of medical collagen powder, add 1000 parts by weight of 0.5mol / L acetic acid solution, stir at low temperature until completely dissolved;

[0044] (2) preparation of chitosan solution

[0045] Take 10 parts by weight of chitosan solid, add 1000 parts by weight of distilled water, stir and dissolve in a water bath at 40° C. to form a uniform solution, and cool it for later use.

[0046] (3) Preparation of the blend

[0047] Add the above collagen solution and chitosan solution into the reaction kettle respectively, stir for 120 minutes to make the blend liquid uniform, and vacuum defoam after filtration.

[0048] (4) Preparation of acellular dermal matrix composite membrane material

[0049] After flattening the qualified decellularized porcine dermal matrix material with a unit area of ​​100, the above-mentioned collagen-chitosan blend solution is evenly coated on the surface of the decellularized...

Embodiment 2

[0053] (1) Preparation of collagen solution

[0054] Get 25 parts by weight of medical collagen powder, add 2000 parts by weight of 0.2mol / L acetic acid solution, stir at low temperature until completely dissolved;

[0055] (2) Preparation of polyvinyl alcohol solution

[0056] Take 10 parts by weight of polyvinyl alcohol solid, add 100 parts by weight of distilled water, stir and dissolve in a water bath at 95°C to form a uniform solution, and cool it for later use;

[0057] (3) preparation of chitosan solution

[0058] Take 15 parts by weight of chitosan solid, add 800 parts by weight of distilled water, stir and dissolve in a water bath at 60° C. to form a uniform solution, and cool it for later use.

[0059] (4) Preparation of the blend

[0060] The above-mentioned collagen solution, polyvinyl alcohol solution, and chitosan solution were respectively added to the reaction kettle, stirred for 120 minutes to make the blend liquid uniform, and vacuum defoamed after filtrat...

Embodiment 3

[0066] (1) Preparation of collagen solution

[0067] Get 56 parts by weight of medical collagen powder, add 5000 parts by weight of 0.2mol / L acetic acid solution, stir at low temperature until completely dissolved;

[0068] (2) Preparation of polyvinyl alcohol solution

[0069] Take 24 parts by weight of polyvinyl alcohol solids, add 160 parts by weight of distilled water, stir and dissolve in a water bath at 95°C to form a uniform solution, and cool it for later use;

[0070] (3) Preparation of the blend

[0071] Add the above collagen solution and polyvinyl alcohol solution into the reaction kettle respectively, stir for 60 minutes to make the blend liquid uniform, and vacuum defoam after filtration. Prepare collagen-polyvinyl alcohol binary blend;

[0072] (4) Preparation of acellular dermal matrix composite membrane material

[0073] After the acellular porcine dermal matrix material with a unit area of ​​100 is flattened, the above-mentioned binary blend solution is e...

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Abstract

The invention discloses an acellular dermal matrix complex film material and a preparation method thereof. The method is characterized in that: 18 to 28 weight portions of collagen is dissolved into a solution by an acetic acid aqueous solution with a concentration between 0.2 and 0.5mmol / L; 10 to 24 weight portions of polyvinyl alcohol and 10 to 40 weight portions of chitosan are respectively dissolved into solutions with different concentrations by distilled water at a temperature of between 85 and 95 DEG C and at a temperature of between 40 and 60 DEG C, and the collagen solution and the polyvinyl alcohol solution and / or the chitosan solution are mixed according to the proportion and then are evenly stirred to prepare a mixed liquid; the mixed liquid is evenly coated on the surface of an acellular dermal matrix material to ensure that the thickness of a film reaches between 0.5 and 2.5cm and the film is quickly frozen, and then the acellular dermal matrix complex film material is produced through freeze drying; and finally carbodiimide is adopted to modify so as to prepare an acellular dermal matrix composite collagen-polyvinyl alcohol and / or chitosan film material.

Description

technical field [0001] The invention relates to a method for preparing a decellularized dermis matrix composite membrane material using animal skin body collagen, hydrolyzed collagen, polyvinyl alcohol (PVA), chitosan, etc. as raw materials. Background technique [0002] Collagen from animal skin not only has unique physical and chemical properties, but also has excellent properties such as good biocompatibility, biodegradability, low immunity and hemostatic performance, especially it is easily absorbed in animals, and is friendly It is considered to be one of the most ideal biomedical materials because of its strong water properties, no toxic side effects, and good safety. Combining collagen with other natural / synthetic macromolecular compounds to make biomedical materials suitable for various purposes has received great attention. [0003] Generally, according to the different forms of collagen, it can be divided into collagen body and hydrolyzed collagen. Collagen body ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/60
Inventor 但卫华林海但年华黄惠兴刘汝诚汤圣
Owner JIANGYIN BENXIANG BIOTECHOLOGY
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