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Method for producing D-ribose by fermenting bacillus subtilis

A technology for Bacillus subtilis and Bacillus subtilis is applied in the field of using Bacillus subtilis to ferment D-ribose, which can solve the problems of low yield and long fermentation period, and achieve the effects of easy industrialization, reduction of by-products, and simple and easy control.

Inactive Publication Date: 2009-01-07
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although people have carried out a lot of research on the production of D-ribose by microbial fermentation for many years, the methods currently used still have the disadvantages of long fermentation period and low yield.

Method used

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  • Method for producing D-ribose by fermenting bacillus subtilis
  • Method for producing D-ribose by fermenting bacillus subtilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method utilizing Bacillus subtilis to ferment and produce D-ribose, the specific steps of which are:

[0030] (1) select transketolase-deficient Bacillus subtilis as bacterial strain;

[0031] (2) strain culture,

[0032] A. The glycerol suspension of the transketolase-deficient Bacillus subtilis EC2 that was cryopreserved was connected to the slant medium, and cultivated in a constant temperature incubator at 37° C. for 40 hours;

[0033] The components of the slant medium are: sorbitol 5g / l, peptone 10g / l, NaCl 2g / l, yeast extract 2g / l, KH 2 PO 4 1g / l, K 2 HPO 4 2g / l, agar 20-22g / l, prepared with deionized water, pH 7.0~7.2.

[0034] B. Under sterile conditions, insert the strains on the slant of the two inoculation loops into a 250mL shake flask equipped with sterilized 20ml seed medium, and cultivate them on a shaker at 220rpm at 37°C for 47 hours, as the primary seed;

[0035] The components of the seed medium are: glucose monohydrate 20g / l, KH 2 PO 4 1g / ...

Embodiment 2

[0041] Shake flask fermentation was carried out according to the method described in Example 1, but in the fermentation medium described in Example 1, 0.2g / l, 0.3g / l, 0.55g / l of citric acid were added respectively to form three groups of experiments Group, after 70 hours of fermentation, compare with the fermentation result of embodiment 1, the results are shown in Table 1:

[0042] Table 1

[0043]

[0044] Experimental results:

[0045] Adding the citric acid shown in the amount can improve the output and yield of D-ribose in the fermentation process of the transketolase-deficient Bacillus subtilis, especially when adding 0.3g / l citric acid, the D-ribose output increases the most, compared with The output of the control group increased by 10.7%, and the yield increased by 10.8%.

Embodiment 3

[0047] Shake flask fermentation was carried out according to the method described in Example 1, but 3 g / l, 4 g / l, 6 g / l, and 7 g / l of lactic acid were added respectively to the fermentation medium described in Example 1 to form four groups of experiments Group, after 70 hours of fermentation, compare with the fermentation result of embodiment 1, the results are shown in Table 2:

[0048] Table 2

[0049]

[0050] Experimental results:

[0051]Adding the indicated amount of lactic acid can improve the output and yield of D-ribose in the fermentation process of transketolase-deficient Bacillus subtilis, especially when adding 6g / l lactic acid, the D-ribose output increases the most, which is higher than that of the control group. Increased by 22.4%, yield increased by 22.5%.

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Abstract

The invention relates to a method for producing D-plant nucleic acid by using Bacillus subtilis to ferment which includes the steps: (1) inoculating the production strain of transketolase defect Bacillus subtilis into a seed culture medium to activate to obtain a seed liquid; (2) preparing a fermentation culture medium which takes dextrose as a carbon source and adding organic acid such as citric acid or lactic acid; (3) inoculating the seed liquid into the fermentation culture medium to carry out fermentation. The method of the invention has the beneficial effects of: being capable of remarkably increasing the yield of the D-plant nucleic acid and improving the yield rate; reducing the outgrowths in the fermentation liquid, thus being beneficial to the separation and extraction of the D-plant nucleic acid; being simple and practical to control the fermentation metabolic process and being easily industrialized.

Description

【Technical field】 [0001] The invention relates to the technical field of synthesizing required compounds by means of fermentation in biotechnology, specifically, a method for producing D-ribose by fermentation of Bacillus subtilis. 【Background technique】 [0002] D-ribose is a five-carbon sugar with important physiological and biochemical functions. It widely exists in the cells of animals, plants and microorganisms. It is a component of genetic material in organisms, such as ribonucleic acid (RNA), several coenzymes and vitamins. [0003] D-ribose has huge economic value and is widely used in food, medical and pharmaceutical fields: ①It can be used to produce feed additives and food seasonings; ②It is a synthetic vitamin B 2 ③ It can be used clinically to treat myocardial ischemia, diabetes, muscle pain and other diseases; ④ It can be used to produce antiviral and anticancer drugs, etc. With the development of the industry and the deepening of applied research, D-ribose wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12R1/125
Inventor 叶勤李志敏吴琳
Owner EAST CHINA UNIV OF SCI & TECH
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