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Labelling foodstuff pathogenic bacteria growth checking method

A detection method and a pathogenic technology, which are applied in the field of rapid detection of tracer bacterial growth conditions, can solve the problems of unsolved key technical problems, limited development and application, etc., and achieve the effect of low selectivity and enhanced accuracy

Inactive Publication Date: 2009-01-07
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are research reports on monitoring food microorganisms with immunosensors, quartz crystal piezoelectric sensors and other biosensors. These methods have some key technical problems that have not been resolved, which limits their development and application.

Method used

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  • Labelling foodstuff pathogenic bacteria growth checking method
  • Labelling foodstuff pathogenic bacteria growth checking method
  • Labelling foodstuff pathogenic bacteria growth checking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Analysis of the growth situation of Staphylococcus aureus

[0033] Inoculate Staphylococcus aureus strains in 1% glucose broth medium, culture statically for 18-24 hours, and prepare seed solution.

[0034] Transfer equal amounts of the seed solution to Erlenmeyer flasks containing 50ml of 1% glucose broth medium, and culture at 37°C for 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h , 11h, 12h, 13h, 24h, and 30h, inactivate the culture medium in a water bath at 100°C for 40min.

[0035] The inactivated culture medium was detected by a multi-frequency large-scale pulse intelligent chemical analysis system. The sensor array uses Wu electrodes, Pt electrodes, Ag electrodes, and Ti electrodes as working electrodes, platinum electrodes as auxiliary electrodes, and Ag / AgCl as reference electrodes.

[0036] The multi-frequency large-scale pulse excitation potential consists of three pulse frequency segments of 1Hz, 10Hz and 100Hz, which are equivalent to large-sc...

Embodiment 2

[0041] Embodiment 2: Escherichia coli O157: H7 growth analysis

[0042] Escherichia coli O157:H7 strains were inoculated in 1% glucose broth medium, and cultured statically for 18-24 hours to prepare seed liquid.

[0043] Transfer the same amount of the seed solution to the Erlenmeyer flasks equipped with 50ml of 1% glucose broth medium, and cultivate them for 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h , 13h, 24h, and 30h later, inactivate the culture medium.

[0044] The inactivated culture medium was detected by a multi-frequency large-scale pulse intelligent chemical analysis system. The sensor array uses Pt electrodes and Au electrodes as working electrodes, platinum electrodes as auxiliary electrodes, and Ag / AgCl as reference electrodes.

[0045] The pulse voltage, pulse frequency, pulse interval time, detection method and signal analysis method are the same as those in Embodiment 1.

[0046] Finally, the 1Hz frequency band of the Pt electrode and the 100Hz ...

Embodiment 3

[0049] Embodiment 3: Analysis of the growth of Bacillus subtilis

[0050] The Bacillus subtilis strain is inoculated in 1% glucose broth medium, and cultured statically for 18-24 hours to prepare a seed liquid.

[0051] The seed solution was transferred in equal amounts to the Erlenmeyer flasks equipped with 50ml 1% glucose broth medium, and cultured for 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, respectively. After 12h, 13h, 24h, and 30h, the culture medium was inactivated.

[0052] The inactivated culture medium was detected by a multi-frequency large-scale pulse intelligent chemical analysis system. The sensor array uses Ba electrode as the working electrode, platinum electrode as the auxiliary electrode, and Ag / AgCl as the reference electrode.

[0053] The pulse voltage, pulse frequency, pulse interval time, detection method and signal analysis method are the same as those in Embodiment 1.

[0054] The 1Hz frequency section, 10Hz frequency section, and 100Hz freq...

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Abstract

The invention discloses a rapid detection method which has the characteristics of low selectivity, no specificity and interactive sensitivity. The method can be used for accurately, sensitively, simply, economically and rapidly tracing the growth of pathogenic bacteria of food and the integrated information of metabolism thereof. In the method, bacteria are inoculated; the bacteria can be used for inactivating culture solution after being cultured for different periods of time; an intelligent chemical analysis system of multi-frequency wide-breadth pulse is used for detecting the inactivated culture; response signals are collected and analyzed; other steps also comprised in the method. The method uses an intelligent multi-frequency pulse scanner based on multi-frequency wide-breadth pulsevoltammetry, and combines a three-electrode system which comprises a working electrode array, so as to collect the integrated information of the growth of pathogenic bacteria of food in the way whichhas the characteristics of low selectivity, no specificity and interactive sensitivity; then the method of principal component analysis is used for processing the collected information so as to tracethe growth and metabolism of the pathogenic bacteria of food in the simple, accurate and sensitive way.

Description

technical field [0001] The invention relates to the technical field of rapid detection of trace bacterial growth, in particular to a method for monitoring the growth of food-borne pathogenic bacteria in a liquid medium by a multi-frequency large-scale pulse intelligent chemical analysis system. Background technique [0002] In recent years, food poisoning and other diseases caused by food pathogenic bacteria have attracted great attention from the society. Escherichia coli O157:H7 (E.coli O157:H7), Staphylococcus aureus (Staphylococcus Aureus), and Salmonella typhimurium (Salmonella typhimurium) are considered to be the most harmful food-borne pathogens. Huge economic losses, but also a serious threat to human health and safety. Therefore, in the process of food production, processing, storage and sales, it is of great significance to guide food safety production and sanitation testing to trace the comprehensive information of the growth status and law of pathogenic bacteri...

Claims

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Application Information

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IPC IPC(8): G01N27/27C12Q1/02
Inventor 赵广英黄建锋邓少平田师一
Owner ZHEJIANG GONGSHANG UNIVERSITY
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