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Red Chinese fevervine extract and medical composition thereof and food composition and preparation method thereof

A medicinal composition and a technology for the red sputum vine, which are applied in the field of the rhizoma striata extract, can solve problems such as a single function of lowering blood sugar or blood lipids, and achieve the effects of improving metabolic syndrome, simple steps, and regulating blood lipids.

Active Publication Date: 2009-02-04
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to overcome the currently used Polygonum Polygoni Multiflori, which is traditional Polygonum Polygoni Multiflori, and only has a single function of lowering blood sugar or blood fat, without any defects related to the treatment of metabolic syndrome, the present invention provides a single use of Polygonum chinensis (also known as Taiwan's Polygonum multiflorum) extract, so that it inhibits the activity of intestinal glycolytic enzymes and acetyl-CoA carboxylase (ACC) in the liver, and promotes the activity of acyl-CoA oxidase (acyl-CoA oxidase, ACO) in the liver ) activity for improving the function of metabolic syndrome

Method used

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  • Red Chinese fevervine extract and medical composition thereof and food composition and preparation method thereof
  • Red Chinese fevervine extract and medical composition thereof and food composition and preparation method thereof
  • Red Chinese fevervine extract and medical composition thereof and food composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1. The preparation of red chicken dung vine extract

[0044] Acid Alcoholic Extract:

[0045] Grind the red chicken dung vine with a grinder and extract it with acidic alcohol (with the addition of 0.1% hydrochloric acid (HCl)). The ratio of the weight of red chicken dung to the volume of the acidic alcohol is about 1:8 (W / V =1 / 8), it can be left standing at room temperature or can be shaken and extracted at a speed of 1000 revolutions (rpm).

[0046] After 72 hours, perform preliminary filtration with gauze, and then centrifuge the obtained filtrate at a rate of 6,000 revolutions per minute (rpm) for 15 minutes. The supernatant is concentrated and dried to become an acidic alcohol extract, and the extraction rate is about 6.9%.

[0047] The extract was then reconstituted to a concentration of 50 mg / ml.

[0048] hot water extract

[0049] Put the red chicken dung vine into a traditional Chinese medicine pot and boil it for about two hours. The weight o...

Embodiment 2

[0051] Example 2. Red chicken dung vine extract inhibits α-glucosidase (α-glucosidase)

[0052] Inhibition of α-glucosidase activity was analyzed by p-nitrophenyl α-D-glucoside (p-nitrophenyl α-D-glucoside, pNP-G) substrate assay. The method is to include:

[0053] 1. First configure the α-glucosidase (EC 3.2.1.20, G5003, Sigma-Aldrich Co., USA) solution of 1U / ml with phosphate buffer solution (100mM, pH 7.0) and 5x10 -3 M p-nitrophenyl-α-D-glucopyranoside (p-nitrophenyl α-D-glucopyranoside, pNP-G, Sigma-Aldrich Co., USA) substrate solution was set aside.

[0054] 2. Then add 40 microliters (μl) of phosphate buffer solution (0.4M, pH 7.0), 10 microliters (μl) of α-glucosidase solution, and different dilutions to each well of the 96-well plate. Deionized water was added to 50 microliters (μl) of the red gallinaceous vine extract to 170 microliters (μl).

[0055] 3. After preheating the incubator at 37°C for 5 minutes, add 30 microliters (μl) of the preheated substrate soluti...

Embodiment 3

[0062] Example 3. The analysis of the activity of different glycolytic enzymes inhibited by the extract of the red chicken dung vine

[0063] Rat intestinal enzyme crude extract (rat intestinal acetone powder, SigmaChemical Co., St.Louis, Mo.) was used as the source of glycolytic enzymes, and different sugar substrates (maltose, sucrose and starch) were given to test the acidity Inhibition of different glycolytic enzyme activities by the alcoholic red chrysanthemum extract.

[0064] Please refer to Table 2, which shows the inhibition of different glycolytic enzyme activities by the acidic alcoholic red chicken dung extract, which also uses IC 50 To represent the situation of activity inhibition:

[0065] Table 2.

[0066]

[0067] As shown in the above table 2, the acid alcohol extract has inhibitory effect on maltase, α-amylase and sucrase, and the inhibition intensity is 100% of that of maltase. >Amylolytic enzymes>Sucrose glycolytic enzymes.

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Abstract

The invention relates to a red Chinese fevervine herb extract used for improving the function of metabolic syndrome and a medical compound as well as a food compound thereof; the invention discloses the red Chinese fevervine herb extract used for improving the function of metabolic syndrome; firstly, a medical compound which contains red Chinese fevervine herb extract and is used for improving the metabolic syndrome is yet to be disclosed and then a food compound which contains the red Chinese fevervine herb extract and is applicable to patients with metabolic syndrome; the invention is prepared by a simple preparation method, and is proved to have the function of adjusting blood sugar and blood fat, as well as restricting the activity of sucroclastic enzyme and carboxylated enzyme of acetyl coenzyme A; the invention can also enhance the activity of oxidizing enzyme of acyl coenzyme A; therefore, the invention can achieve the effect of improving the metabolic syndrome by only utilizing the single red Chinese fevervine herb extract.

Description

technical field [0001] The present invention is an extract of red chicken dung vine, especially one that can inhibit the activity of intestinal glycolytic enzymes and acetyl-CoA carboxylase (acetyl-CoA carboxylase, ACC) in the liver, and promote the activity of acyl-CoA carboxylase in the liver. A oxidase (Acyl-CoA oxidase, ACO) activity, to improve metabolic syndrome of red chicken dung vine extract. Background technique [0002] U.S. Patent No. 5,531,991 discloses a "composition and method for treating hyperglycemia using Polygonum multiflorum extract", which is an extract obtained by extracting Polygonum multiflorum with 0.1N ammonium hydroxide Polygel G-25 (SephadexG-25) is divided into 3 fractions, and Polygonum Polygonum extract has similar effects of increasing insulin activity and regulating blood sugar. [0003] In U.S. Patent No. 6,200,569, a "composition and method for increasing insulin activity" is disclosed, which uses Polygonum multiflorum and bacteria from t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A23L1/29A61K36/74A61P3/10A61P3/06A23L33/105
Inventor 陈纪桦陈秀雯张源义刘文凯
Owner FOOD IND RES & DEV INST
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