Method for extracting calcium chondroitin sulfate in shark cartilage

Abstract

A method for extracting shark calcium chondroitin sulfate from a shark cartilage comprises the processes in sequence such as curing, alkalization, enzymatic hydrolysis, deproteinization, crystallization, resin pretreatment, resin exchange, ultrafiltration dehydration, calcification of filtrate and crystallization dehydration dying. Because secondary crystallization is adopted, the covalent binding of protein and shark chondroitin sulfate is broken by hydrogen peroxide to enable the chondroitin sulfate in the protein to be dissociated, impurities such as the protein and the like can be effectively removed to improve the purity and the content of shark sodium chondroitin sulfate; cation resin is adopted to complete the sodium removal process by column chromatography, the exchanged shark chondroitin sulfate is high in concentration and the shark chondroitin sulfate which is produced during ion exchange can be accurately controlled, and the sodium content in the product is low; with dehydration of ultrafiltration, not only the concentration can be realized, but also the small molecular impurities can be removed to improve the purity of the shark chondroitin sulfate, at the same time, to improve the content of calcium. The method also can save the using amount of ethanol and reduce the production cost.

Description

technical field [0001] The invention relates to a preparation method of shark chondroitin sulfate calcium, in particular to a method for extracting shark chondroitin sulfate calcium from shark cartilage Background technique [0002] As an important part of connective tissue, chondroitin sulfate has a variety of pharmacological effects and biological functions. It is often used as a dietary supplement, mainly for the adjuvant treatment of osteoarthritis. The traditional methods of extracting chondroitin sulfate mainly include alkaline extraction enzymatic hydrolysis, alkaline extraction, ultrasonic-assisted method and acetic acid extraction method. The chondroitin sulfate prepared by the traditional method mainly exists in the form of sodium salt. The affinity is stronger than sodium. After use, it often causes calcium accumulation in blood vessels and capillaries, resulting in petechiae and hematoma. The common alkaline production process also has the defects of low purity a...

AI Technical Summary

Problems solved by technology

The traditional methods of extracting chondroitin sulfate mainly include alkaline extraction enzymatic hydrolysis, alkaline extraction, ultrasonic-assisted method and acetic acid extraction method. The chondroitin sulfate prepared by the traditional method mainly exists in the form of sodium salt. The affinity is stronger than sodium. After use, it often causes calcium accumulation in blood vessels and capillaries, resulting in petechiae and hematoma. The common alkaline production process also has the defects of low purity and high production cost due to the large amount of ethanol used.