Quantum point biological probe and preparation method thereof, and microcurrent control protein chip based on the same
A technology of protein chips and biological probes, which can be used in biological testing, measuring devices, material inspection products, etc., and can solve the problems that chips have not made substantial progress.
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Embodiment 1～3
[0022] 1. Preparation of water-soluble CdTe quantum dot polyacrylic acid nano-microspheres (prepared with reference to CN1912047A): (1) preparation of potassium hydride telluride: 110.5 mg of KBH 4 Put the solid and 91.6 mg of Te powder into a small flask, add 3 ml of water, and react at 0 degrees Celsius for 20 hours to obtain a KHTe solution for subsequent use; (2) Preparation of CdTe precursor solution: 36.8 mg of CdCl 2 Dissolve in 100 ml of water, add 0.05 ml of mercaptoacetic acid, adjust pH=11.0 with 0.5 mol / L NaOH solution, inject 0.30 ml of KHTe solution as CdTe precursor solution; (3) cadmium telluride CdTe precursor containing polymer Solution preparation: 5 ml of polyacrylic acid (Mw=2,000) solution with a concentration of 0.00001mol / L was added to the CdTe precursor solution, stirred for 20 minutes, and prepared as a cadmium telluride CdTe precursor solution containing a polymer; (4) Microwave radiation Preparation of polymer-wrapped CdTe quantum dots: The obtaine...
Embodiment 4～6
[0028] 1. Preparation of water-soluble CdTe / CdS quantum dot polyacrylamide nanospheres (prepared with reference to CN1693207A and CN1912047A): (1) Preparation of sodium telluride hydride: 92.7 mg of NaBH 4 Solid and 127.6 milligrams of Te powder were put into a small flask, 3 milliliters of water was added, and after reacting for 7 hours at 15 degrees Celsius, NaHTe solution could be obtained for subsequent use; (2) preparation of CdTe quantum dots: 22.5 milligrams of CdCl 2 Dissolve in 100 ml of water, add 0.03 ml of mercaptoacetic acid, adjust pH=9.5 with 0.5 mol / L NaOH solution, pass nitrogen gas for 30 minutes, heat up to 100 degrees Celsius, inject 0.2 ml of NaHTe solution, and react for 3 hours to obtain CdTe quantum dot solution ; (3) Preparation of CdTe / CdS precursor solution: 18.5 mg of CdCl 2 and 5.6 mg Na 2 Dissolve S in 100 ml of water, add 0.06 ml of thioglycolic acid, adjust the pH to 10.5 with 0.5 mol / L of NaOH, inject 10 ml of CdTe quantum dot solution, and ob...
Embodiment 7
[0033] Bidirectional microfluidic channels perpendicular to each other are prepared by using polydimethylsiloxane (PDMS). For the method, please refer to the patent application document of the inventor, and the application number is CN200610117234.X. The microchannel and the chip substrate are reversibly sealed by a physical method.
[0034] 1. Monoclonal antibodies (commercially purchased from FITZGERALD, USA) of three proteins, including thrombin, platelet growth factor and immunoglobulin E, were immobilized on the chip reaction area through three independent microfluidic channels in the same direction. The concentrations were all 150mg / L, and post-treatments such as washing and blocking were carried out after 2 hours.
[0035] 2. Three kinds of target proteins, such as thrombin, platelet growth factor and immunoglobulin E, whose concentrations are 20pg / ml, 50pg / ml, 100pg / ml, 200pg / ml and 500pg / ml, pass through the direction perpendicular to the direction of the microchannel...
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