Method for extracting high-purity epigallocatechin-3-gallate from green tea

A technology of epigallocatechin and gallocatechin, which is applied in the field of extracting and purifying high-purity epigallocatechin gallate, can solve the problems of high price and expensive chemical reagents, and achieve high economic utilization Value, broad market prospects, and high product purity

Inactive Publication Date: 2009-03-11
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, there are still some problems. One is the use of more expensive instruments and expensive chemical reagents, and the other is the production of a large amount of waste liquid that pollutes the environment. As a result, the international price of EGCG has remained high. Therefore, in order to reduce costs, explore cheap EGCG separation The extraction method has important theoretical significance and application value

Method used

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  • Method for extracting high-purity epigallocatechin-3-gallate from green tea
  • Method for extracting high-purity epigallocatechin-3-gallate from green tea
  • Method for extracting high-purity epigallocatechin-3-gallate from green tea

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Embodiment Construction

[0022] The present invention will be further described in detail through specific examples below.

[0023] (1) Grind the green tea, take 25 grams of tea powder, and put it in a 500ml beaker.

[0024] (2) Add 350ml of double-distilled water, boil for 20-30 minutes, stir once every minute, and filter with suction to obtain tea soup. Add the tea dregs to 350ml of double-distilled water and boil for 20-30 minutes, then filter the tea soup to get the tea soup, combine two tea soups, and the EGCG content detected by high performance liquid chromatography is 30.81% (such as figure 2 ).

[0025] (3) Put the tea soup in a 1000ml large beaker, keep it warm in a 70°C water bath, add caffeine to 30mmol / L, cool it at room temperature for 1-4 hours after complete dissolution, and cool at 4°C for 0.5-2.0 hours. Centrifuge at 15000g for 30 minutes at 4°C, discard the supernatant, take the precipitate, take 300ml of redistilled water at 60°C to dissolve the precipitate, and transfer the sol...

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Abstract

The invention relates to a technology and a method for extracting high-purity Epigallocatechin gallate (EGCG) and Epicatechin-3-gallate (ECG) from green tea. The green tea is common green tea in the market; after combined purification of the green tea through caffeine deposit, liquid-phase extraction and scintillation silica gel column chromatography technology, the high-purity EGCG can be obtained, and the purity of the EGCG after purification can reach more than 97 percent; the concentration of the caffeine deposit is between 20 and 50 mmol / L; a liquid-phase extractant is chloroform and ethyl acetate; filler of a scintillation silica gel chromatographic column is silica gel G (between 200 and 300 meshes), wherein the length of the column is 30 centimeters and the inside diameter is 2.0 centimeters; the ethyl acetate and petroleum ether (between 60 and 90 DEG C) are taken as eluant, and the volume ratio of the ethyl acetate to the petroleum ether is 3 to 2; 0.4 to 0.8 percent of trifluoroacetic acid is added; various reagents are general reagents and are economic and easy to purchase; and except the chloroform, other reagents are safe and nontoxic, and the chloroform is easy to remove. The method can be applied to industrial mass production of the high-purity EGCG and the ECG.

Description

(1) Technical field [0001] The invention relates to a method for extracting epigallocatechin gallate (EGCG) from green tea, in particular to a method for extracting and purifying high-purity epigallocatechin gallate from green tea. The method can be applied to industrial extraction of high-purity epigallocatechin gallate. (2) Background technology [0002] Tea is one of the traditional beverages in China. It has multiple functions such as anti-cancer, anti-oxidation, anti-atherosclerosis, anti-senile dementia, and immune function regulation. Its main active ingredients are catechins in tea polyphenols. Epigallocatechin gallate (EGCG) is the component with the highest content and the strongest activity among catechin components, accounting for 50% to 60% of tea polyphenol products. The ester formed by pyran and gallic acid has the versatility of phenolic antioxidants. At the same time, it has many properties superior to other catechins because of its 6 ortho-phenolic hydroxy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 周杰王进朱树华陈长宝高吉刚
Owner SHANDONG AGRICULTURAL UNIVERSITY
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