Deoxyhydroxylputrescinelysine synthase code gene and antisense base sequences thereof

An antisense nucleotide and nucleotide sequence technology, applied in the field of deoxyhydroxyputrescine lysine synthase coding gene and its inhibition of gene expression, can solve crop yield reduction, global food and ecological crisis, and affect crop growth conditions and other issues to achieve the effect of increasing production and reducing costs
CN101386842BInactive Publication Date: 2012-06-13BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Publication Date
2012-06-13
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention discloses an encoding gene of deoxyhypusine synthase and a method for inhibiting expression of the gene and a special antisense nucleotide sequence thereof. The deoxyhypusine synthase of the invention is selected from (a) or (b): (a) a protein which consists of amino acid sequence showed in sequence 4 of the sequence table; (b) a protein which is derived from (a) by substituting and / or deleting and / or adding one or more than one amino acid residue of the amino acid sequence showed in sequence 4 of the sequence table, and encodes the deoxyhypusine synthase. The invention uses antisense nucleotide sequence of DHS to inhibit normal expression of plant endogenous DHS gene, thereby the invention improves the resistance capability of plants against different stress environments, such as drought resistance, low temperature tolerance and salt tolerance. Each resistance is significantly higher than the negative control and the resistances are improved at the same time. Therefore,the encoding gene of the deoxyhypusine synthase and the antisense nucleotide sequence thereof and the method thereof have important significance on improving crop yield.
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Description

technical field

[0001] The present invention relates to the coding gene of deoxy putrescine lysine synthase and its antisense nucleotide sequence, in particular to the coding gene of deoxy putrescine lysine synthase, the method for inhibiting the expression of the gene and the special antisense nucleoside acid sequence. Background technique

[0002] The growth and development of plants are greatly affected by the environment. All kinds of environmental stress will seriously affect the growth of crops, resulting in crop yield reduction, leading to global food and ecological crisis. In today's global shortage of food supply, it is especially necessary to improve the resistance of crops to overcome various adverse environments, such as low temperature, high temperature, drought, and salinity, so as to increase crop yields to alleviate food pressure.

[0003] With the development of molecular biology, people have been able to understand the mechanism of plant tolerance (resist...

Claims

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