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Detection kit for schistosomiasis japonica blood serum designated object

A serum marker and primary liver cancer technology, applied in the field of biomedical diagnostics, can solve problems such as early warning, early diagnosis and curative effect evaluation of unseen liver cancer

Inactive Publication Date: 2009-03-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been used as a detection index for early warning, early diagnosis and curative effect evaluation of primary liver cancer

Method used

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  • Detection kit for schistosomiasis japonica blood serum designated object
  • Detection kit for schistosomiasis japonica blood serum designated object
  • Detection kit for schistosomiasis japonica blood serum designated object

Examples

Experimental program
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Effect test

preparation example Construction

[0040] 2. Preparation of the kit

[0041] 1. Preparation of antigen-coated microtiter plates

[0042] 1) Prepare coating solution: take sodium bicarbonate (NaHCO 3 ) 2.9g, sodium carbonate (Na 2 CO 3 ) 1.6g, sodium azide (NaN 3 ) 0.2g, dissolved in 400ml of deionized water, adjusted to pH 9.6, then dilute to 500ml, set aside at 4°C. This is 0.05M carbonate buffer.

[0043] 2) Preparation of polypeptide antigen solution: 10 kinds of polypeptides with a purity greater than 95% were dissolved in the coating solution, mixed well, and the concentration was 10 μg / ml.

[0044] 3) Enzyme plate coated: except for L7B which is a separate antigen solution, the other 4 groups of antigen solutions are mixed in equal proportions and added to the corresponding microwells of the plate according to the labels of each group, 100 μl / well, covered, Place at 37°C for 2 hours, then at 4°C overnight.

[0045] 4) Blocking: Aspirate the polypeptide antigen solution from each well, then add bloc...

Embodiment 1

[0055] Example 1: A detection kit for serum markers of primary liver cancer

[0056] The composition of kit of the present invention is as follows:

[0057] 1. One piece of antigen-coated enzyme-labeled plate, which is composed of 10 enzyme-labeled strips of 1×8 wells, and each 2 strips is a group, which is divided into 5 groups, respectively marked as URG4, URG7, and URG11 , S15a and Sui1, to correspond to the corresponding antigens coated, see figure 1 . For example, the enzyme label marked URG4 is coated with L4A and L4B; the one marked URG7 is coated with L7-B; the one marked URG11 is coated with L11-1, L11-2 and L11-3 ; Labeled S15a, coated with L12-A and L12-B; labeled Sui1, coated with Sui1-A and Sui1-B.

[0058] 2. One bottle of enzyme-labeled antibody working solution, containing 20ml of enzyme-labeled antibody working solution.

[0059] 3. One bottle of sample diluent, containing 20ml of sample diluent.

[0060] 4. One bottle of washing solution A, containing 50...

Embodiment 2

[0069] Embodiment 2: Use the kit of the embodiment of the present invention 1 to detect the serum sample of the patient to be tested

[0070] 1. Label the antigen-coated ELISA plates sequentially with URG4, URG7, URG11, S15a and Sui1, and fix them with adhesive tape.

[0071] 2. Take 1 copy of negative control serum and 1 positive control serum, 14 samples of serum samples from patients to be tested, 16 serum samples in total, add 75 μl of sample diluent to each well of the microtiter plate, and then separate the samples from each of the 16 serum samples according to the group. Add 25 μl into the well, mix well, cover, and incubate at 37°C for 45min.

[0072] 3. Dilute 50ml of detergent A to 900ml with deionized water, add 1ml of detergent B, mix well and set the volume to 1000ml, wash each well 5 times for 3 minutes each time, and pat dry.

[0073] 4. Add 100 μl of enzyme-labeled antibody working solution to each well, cover it, and incubate at 37°C for 45 minutes. After tak...

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Abstract

The invention relates to the technical field of biomedical diagnostics and discloses a reagent kit for detecting a serum marker of primary hepatic carcinoma, which consists of an ELISA plate enveloped by antigens, an enzyme-labeled antibody working solution, a sample diluent, a washing liquid, positive and negative control serum, a chromogenic solution and a stopping liquid. The reagent kit is characterized in that the envelope antigens of the ELISA plate are five groups of peptide antigens L4-A and L4-B; L7-B; L11-1, L11-3 and L11-4; L12-A and L12-B; and Sui1-A and Sui1-B, which correspond to five serum marker antibodies of the primary hepatic carcinoma; and Sui1-A and Sui1-B respectively. Except the L7B which is separately enveloped, each group of all the other groups of peptide antigens is enveloped by proportionally mixing polypeptides in the same group. Proven by evaluation experiments of the reagent kit and clinical trial, the reagent kit has good specificity and sensitivity, and can be used for early warning prompt or early diagnosis before the primary hepatic carcinoma appears or at early stage of the primary hepatic carcinoma, thereby improving the survival rate of carcinoma patients and evaluating treatment effect and disease outcome in time by observing the dynamic change of related indicators of the serum.

Description

technical field [0001] The invention relates to the technical field of biomedical diagnostics, and is a kit for detecting serum markers of primary liver cancer. Background technique [0002] Primary hepatocellular carcinoma (HCC) ranks high in the death rate of malignant tumors, which seriously threatens human life and health. The number of deaths from liver cancer in my country accounts for about 40% of the death toll of liver cancer in the world every year. The main reason for the high incidence of liver cancer is that about 10% of the population in my country is positive for hepatitis B surface antigen (HBsAg), and those who are positive for HBsAg or have a history of chronic liver disease are the main high-risk groups for liver cancer. At present, the serological cancer marker alpha fetoprotein (AFP), which is used for the diagnosis of primary liver cancer, has a low sensitivity and positive rate for the diagnosis of primary small liver cancer, which are 33%-65% and 9....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/543
Inventor 王文费迈科戚中田
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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