Method for directly purifying tetracycline in water-containing sample by using molecularly imprinted polymer

A molecular imprinting and polymer technology, which is applied in chemical instruments and methods, water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of no reporter imprinted polymer, etc. Good, high-capacity effect

Inactive Publication Date: 2009-04-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method introduced in this article relies on the physical and chemical properties of the template antibiotic, and there is no report applicable to molecularly imprinted polymers for other antibiotics.
For antibiotic detection and treatment in aqueous samples, so far there are no reports involving metal ion-mediated molecularly imprinted polymers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of Tetracycline Molecularly Imprinted Polymer:

[0031] Tetracycline template 0.1mmol, functional monomer methacrylic acid (MAA), FeSO mediated imprinting 4Mix with the cross-linking agent ethylene glycol dimethacrylate (EGDMA) at a molar ratio of 1:1:4:20, dissolve it in 4.5ml of porogen (methanol:water=9:1), and put it into an ampoule 10 mg of initiator azobisisobutylcyanide (AIBN) was added to the medium; ultrasonication was performed for 5 minutes, and nitrogen gas was flushed for 5 minutes, and the seal was sealed under the protection of nitrogen gas, and a tetracycline molecularly imprinted polymer was obtained in a water bath at 60° C. for 24 hours. Take out the aggregated polymer, add ethanol and crush it with a glass rod, wash to obtain 0.1-1.5 μm particles, suspend with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 72 hours, and ...

Embodiment 2

[0038] 1. Preparation of Tetracycline Molecularly Imprinted Polymer:

[0039] Tetracycline template 0.1mmol, functional monomer methacrylic acid (MAA), FeSO mediated imprinting 4 Mix with the cross-linking agent ethylene glycol dimethacrylate (EGDMA) at a molar ratio of 1:2:6:20, dissolve it in 5ml of porogen (methanol:water=9:1), and put it into an ampoule , adding 10 mg of initiator azobisisobutylcyanide (AIBN); ultrasonication for 5 minutes, then flushing with nitrogen for 5 minutes, sealing under nitrogen protection, and 60° C. water bath for 24 hours to obtain a tetracycline molecularly imprinted polymer. Take out the aggregated polymer, add ethanol and crush it with a glass rod, wash to obtain 0.1-1.5 μm particles, suspend with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 64 hours, and the solution was changed every 8 hours. After dehydration is determi...

Embodiment 3

[0046] 1. Preparation of Tetracycline Molecularly Imprinted Polymer:

[0047] Tetracycline template 0.1mmol, functional monomer methacrylic acid (MAA), FeSO mediated imprinting 4 Mix with the cross-linking agent ethylene glycol dimethacrylate (EGDMA) at a molar ratio of 1:2:8:20, dissolve it in 6ml of porogen (methanol:water=9:1), and put it into an ampoule , add initiator azobisisobutylcyanide (AIBN) 10 mg; ultrasonic for 5 minutes, then flush with nitrogen for 5 minutes, seal under nitrogen protection, and bathe in 60°C water for 36 hours to obtain tetracycline molecularly imprinted polymer. Take out the aggregated polymer, add ethanol and crush it with a glass rod, wash to obtain 0.1-1.5 μm particles, suspend with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 72 hours, and the solution was changed every 8 hours. After dehydration is determined by HPLC, the ...

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Abstract

The invention relates to a method for directly purifying tetracycline in samples containing water by utilizing molecular imprinted polymer in the technical field of biotechnology. The steps are: the template molecule of tetracycline, the functional monomer of methacrylic acid, mediated imprinted metal ions and the crosslinker of ethylene glycol dimethacrylate are mixed and dissolved in a pore-generating agent, and an evocating agent is added, the mouth is sealed, after the compositive polymer is mashed, the minute grains are removed, the template molecule is removed by extraction, the polymer in which the template molecule is removed is dried and stays overnight, and the molecular imprinted polymer of tetracycline is obtained; the molecular imprinted polymer of tetracycline is filled in a solid-phase extraction pillar canal; the solid-phase extraction pillar canal is activated and pre-treated, a urine sample treated by KH2PO4 is added, and the tetracycline is leached and purified through the solid-phase extraction pillar canal. The method is characterized by high selection, high specificity, harmful environment resistance and the direct application in water environment.

Description

technical field [0001] The invention relates to a method in the technical field of biochemical engineering, specifically, a method for directly purifying tetracycline in water-containing samples by using molecularly imprinted polymers. Background technique [0002] Tetracycline (Tetracycline, referred to as TC) belongs to the tetracycline antibiotics, is a widely used drug shared by humans and animals, often used to treat and prevent infectious diseases or as a feed additive to promote animal growth. May cause residue if used incorrectly. Harm or pollute people, animals and the environment. Long-term consumption of animal foods with tetracycline exceeding the standard will have direct toxic and side effects and cause allergic reactions to the human body. In order to ensure the health of the people, countries around the world, such as the European Union, Japan, the United States and the Ministry of Agriculture of my country, have made strict regulations on the use and resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/58
Inventor 张大兵武爱波瞿国润
Owner SHANGHAI JIAO TONG UNIV
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