Method for directly purifying tetracycline in water-containing sample by using molecularly imprinted polymer
A molecular imprinting and polymer technology, which is applied in chemical instruments and methods, water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problem of no reporter imprinted polymer, etc. Good, high-capacity effect
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Embodiment 1
[0030] 1. Preparation of Tetracycline Molecularly Imprinted Polymer:
[0031] Tetracycline template 0.1mmol, functional monomer methacrylic acid (MAA), FeSO mediated imprinting 4Mix with the cross-linking agent ethylene glycol dimethacrylate (EGDMA) at a molar ratio of 1:1:4:20, dissolve it in 4.5ml of porogen (methanol:water=9:1), and put it into an ampoule 10 mg of initiator azobisisobutylcyanide (AIBN) was added to the medium; ultrasonication was performed for 5 minutes, and nitrogen gas was flushed for 5 minutes, and the seal was sealed under the protection of nitrogen gas, and a tetracycline molecularly imprinted polymer was obtained in a water bath at 60° C. for 24 hours. Take out the aggregated polymer, add ethanol and crush it with a glass rod, wash to obtain 0.1-1.5 μm particles, suspend with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 72 hours, and ...
Embodiment 2
[0038] 1. Preparation of Tetracycline Molecularly Imprinted Polymer:
[0039] Tetracycline template 0.1mmol, functional monomer methacrylic acid (MAA), FeSO mediated imprinting 4 Mix with the cross-linking agent ethylene glycol dimethacrylate (EGDMA) at a molar ratio of 1:2:6:20, dissolve it in 5ml of porogen (methanol:water=9:1), and put it into an ampoule , adding 10 mg of initiator azobisisobutylcyanide (AIBN); ultrasonication for 5 minutes, then flushing with nitrogen for 5 minutes, sealing under nitrogen protection, and 60° C. water bath for 24 hours to obtain a tetracycline molecularly imprinted polymer. Take out the aggregated polymer, add ethanol and crush it with a glass rod, wash to obtain 0.1-1.5 μm particles, suspend with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 64 hours, and the solution was changed every 8 hours. After dehydration is determi...
Embodiment 3
[0046] 1. Preparation of Tetracycline Molecularly Imprinted Polymer:
[0047] Tetracycline template 0.1mmol, functional monomer methacrylic acid (MAA), FeSO mediated imprinting 4 Mix with the cross-linking agent ethylene glycol dimethacrylate (EGDMA) at a molar ratio of 1:2:8:20, dissolve it in 6ml of porogen (methanol:water=9:1), and put it into an ampoule , add initiator azobisisobutylcyanide (AIBN) 10 mg; ultrasonic for 5 minutes, then flush with nitrogen for 5 minutes, seal under nitrogen protection, and bathe in 60°C water for 36 hours to obtain tetracycline molecularly imprinted polymer. Take out the aggregated polymer, add ethanol and crush it with a glass rod, wash to obtain 0.1-1.5 μm particles, suspend with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 72 hours, and the solution was changed every 8 hours. After dehydration is determined by HPLC, the ...
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