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Method of preparing microbial source stress resistance

A microbial source and endolysate technology, which is applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc. loss, etc.

Inactive Publication Date: 2009-04-08
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term, extensive, large-scale and continuous use of chemical pesticides, on the one hand, leads to the development of resistance of harmful organisms, and forms a vicious circle as the amount of pesticides increases; Cause serious damage, soil deterioration, agricultural by-products and livestock products pesticide residues seriously exceed the standard, not only endanger human health, but also cause great economic losses

Method used

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  • Method of preparing microbial source stress resistance
  • Method of preparing microbial source stress resistance
  • Method of preparing microbial source stress resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Preparation process

[0061] 1.1 Source of raw materials

[0062] The waste product of citric acid fermentation factory-Aspergillus niger.

[0063] 1.2 Preparation method

[0064] 1.2.2 Preparation of lysate enzymatic hydrolysate in the mycelium cell wall

[0065] Add water to the Aspergillus niger in a ratio of 1:5, crush and homogenize the DSH150 high-shear colloid mill (produced by Qidong Dongsheng Chemical Machinery Factory) to break the Aspergillus niger mycelium. The broken tissue is less than 5μm. The homogenate of Aspergillus niger is heated to 55℃, and 1% cellulase (20,000 units / g), 1% pectinase (20,000 units / g), 2% β-glucanase (30,000 units) are added. / G), 2% acid protease (50,000 units / g), fully mixed and incubated at 55°C for 6 hours, then heated to 100°C, held for 10 minutes to inactivate the enzyme, and cooled by a tube centrifuge at 15000 rpm Centrifuge at a high speed at a high speed, take the centrifugal supernatant for use, and use the mycelial residue...

Embodiment 2

[0069] 1. Preparation process

[0070] 1.1 Source of raw materials

[0071] The waste product of citric acid fermentation factory-Aspergillus niger.

[0072] 1.2 Preparation method

[0073] 1.2.2 Preparation of lysate enzymatic hydrolysate in the mycelium cell wall

[0074] Add water to the Aspergillus niger in a ratio of 1:5, crush and homogenize the DSH150 high-shear colloid mill (produced by Qidong Dongsheng Chemical Machinery Factory) to break the Aspergillus niger mycelium. The broken tissue is less than 5μm. The homogenate of Aspergillus niger is heated to 55℃, and 1% cellulase (20,000 units / g), 1% pectinase (20,000 units / g), 2% β-glucanase (30,000 units) are added. / G), 2% acid protease (50,000 units / g), fully mixed and incubated at 55°C for 6 hours, then heated to 100°C, held for 10 minutes to inactivate the enzyme, and cooled by a tube centrifuge at 15000 rpm Centrifuge at a high speed at a high speed, take the centrifugal supernatant for use, and use the mycelial residue...

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Abstract

The invention provides a method for preparing a microbial-source adverse resisting agent. The method comprises the following steps: aspergillus niger thalli is mixed with water in the proportion of 1:5; after the aspergillus niger thalli is ground for homogenate by a high-shear colloid mill, an aspergillus niger mycelium body is broken; a tissue tissue is less than 5 mu m; aspergillus niger thalli homogenate liquid is heated to 55 DEG C; 1 percent of cellulase, 1 percent of pectinase, 2 percent of beta-dextranase and 2 percent of acid protease are added to the homogenate liquid; the materials are well mixed with the homogenate liquid, subjected to heat preservation and hydrolysis for 6 hours at 55 DEG C, heated to 100 DEG C, kept for 10 minutes for killing enzyme, cooled and then subjected to high-speed centrifugation via a tubular centrifuge at the rotating speed of 15,000 rpm; and centrifugal supernatant is taken on standby. According to the weight ratio of (50-60):(2-3):(1-2):(3-5), the supernatant, xylo-oligosaccharide, chitosan oligosaccharide and fulvic acid are well mixed so as to completely dissolve additive substances in the supernatant, are subjected to ultrahigh-temperature instantaneous sterilization at 142 DEG C for threes seconds, and then are canned aseptically, so as to be prepared into finished products.

Description

(1) Technical field [0001] The invention relates to a preparation method of a microorganism-derived anti-stress agent, which belongs to the processing technology of biological pesticides in the field of biological engineering. (2) Background technology [0002] Chemical pesticides play a huge role in the prevention and control of crop diseases, pests and weeds, and promote the development of modern agriculture. Chemical pesticides generally have the advantages of low cost, simple operation, good control effect, quick effect, wide control spectrum, stable properties, and easy storage and transportation. In the past 100 years, they have been the fastest growing and most widely used pesticide varieties in the field of plant protection. However, the long-term, extensive, large-scale, and continuous use of chemical pesticides, on the one hand, leads to the development of resistance to harmful organisms. With the increasing amount of pesticides, a vicious circle is formed; It caused se...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P21/00A01C1/06
Inventor 陶波滕春红李明
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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