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Integrated method for cyclic utilization with microorganism biomass in production process of 2,3-butanediol

A butanediol and product technology, applied in the field of microbiology, can solve the problems of waste bacteria environment, waste of resources and energy, loss of carbon and nitrogen sources and other biomass resources

Inactive Publication Date: 2009-04-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermentative production of 2,3-butanediol by Serratia marcescens has made great progress, but the discharge of a large amount of waste bacteria produced in the fermentation production process is not only a serious environmental problem, but also leads to a large amount of carbon source nitrogen The loss of biomass resources such as energy sources has brought huge pressure on energy conservation and emission reduction
[0011] The existing methods for recycling the soluble components of bacteria are all effective methods for resource recycling, but this process often includes indispensable stages such as wall breaking and dissolution, filtration, separation and even drying, which will also cause waste of resources and energy. Realize the ultimate goal of energy saving and emission reduction; and the examples of recycling live bacteria are relatively few at present, therefore, recycling live bacteria to improve product yield, improve the utilization rate of biomass resources and reduce energy consumption is an urgent need for research in this field new approach to development

Method used

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  • Integrated method for cyclic utilization with microorganism biomass in production process of 2,3-butanediol
  • Integrated method for cyclic utilization with microorganism biomass in production process of 2,3-butanediol
  • Integrated method for cyclic utilization with microorganism biomass in production process of 2,3-butanediol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1. the cultivation of Serratia marcescens

[0068] Said Serratia marcescens (Serratia marcescens) is provided by China Industrial Microorganism Culture Collection Management Center, and the number is Serratia marcescens CICC10187.

[0069] (1) Seed cultivation

[0070] The seeds preserved in glycerol tubes were cultured on a shaker for 24 hours, with one transfer every 12 hours, and the culture temperature was 30° C., 190 r / min.

[0071] The components and contents of the culture medium are (content per liter): glucose 10g; yeast powder 1g; peptone 2g; (NH 4 ) 2 SO 4 6g;K 2 HPO4 10g; NaCl 0.5g; MgSO 4 0.5g; after sterilization, adjust the pH to 6.6 with NaOH solution before inoculation. The balance is water.

[0072] (2) Fermentation culture

[0073] 3.7L bioreactor culture: the seed liquid is inserted in the fermentation medium with the inoculation amount of 5% (volume ratio) and cultivated, and the fermentation medium is (content in every liter):...

Embodiment 2

[0078] Example 2. Feasibility Analysis of Thalline Recovery and Utilization and Optimization of Adding the Amount of Fermentation Recovery Thalline

[0079] By fermentation experiment, insert the living thalline (being reclaimed thalline) of the fermentation end stage that inserts 3.7L bioreactor culture in the blank fermented liquid, insert different amounts of reclaimed thalline in the research fermentation process, and compare with control group ( Whether there are significant differences in the yield (g / l), sucrose utilization (g / g) and product yield (g / l*h) of the fermentation product (g / l) without adding the recovered cells), and examine whether the addition of different cells The impact of volume on each indicator.

[0080] Experimental Materials:

[0081] (1) Seed medium (content per liter): glucose 10g, yeast powder 1g, peptone 2g, (NH 4 ) 2 SO 4 6g, KH 2 PO 4 10g, NaCl 0.5g, MgSO 4 0.5g. The balance is water.

[0082] (2) Shake flask fermentation medium (...

Embodiment 3

[0103] Embodiment 3. Recover the comparison of thalline adding opportunity

[0104] Through the shaking table experiment, the fermented liquid was inserted into live bacteria in different fermentation stages, and the output (g / l) of the fermentation product, the utilization rate of sucrose (g / g), and the productive rate of the product (g / l) were compared between each group. *h) Whether there is a significant difference, and determine the best time to inoculate live bacteria among several groups.

[0105] Experimental Materials:

[0106] (1) Seed medium (content per liter): glucose 10g, yeast powder 1g, peptone 2g, (NH 4 ) 2 SO 4 6g, KH 2 PO 4 10g, NaCl 0.5g, MgSO 4 0.5g. The balance is water.

[0107] (2) Shake flask fermentation medium (content per liter): 90g sucrose, 20g peptone, 5g yeast powder, 10g citric acid, MnSO 4 0.1g, KH 2 PO 4 0.5g, FeSO 4 0.02g, MgSO 4 0.5g. The balance is water.

[0108] experimental method:

[0109] Group 1, Group 2, Group ...

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Abstract

The invention relates to an integration method of the production process of 2,3-butanediol and the recycling of microbial biomass, and the integration method comprises: the 2,3-butanediol is produced by the fermentation of the serratia marcescens seed bacteria; the recovered serratia marcescens is added in a fermentation culture medium of the second fermentation for carrying out the further fermentation culture. The serratia marcescens in fermentation products can be repeatedly utilized. The use of the method can not only improve the yield, the conversion rate and the production ability of the 2, 3-butanediol, but also effectively solve the thallus pollution and the recycling problem of resources, save the energy and reduce the emission.

Description

technical field [0001] The invention belongs to the field of microbiology, and in particular relates to a method for producing 2,3-butanediol by fermenting recovered Serratia marcescens. Background technique [0002] 2,3-butanediol (2,3-butanediol, referred to as BD, the same below) is widely used in various fields such as chemical industry, food and aerospace, and its calorific value is 27200kJ / kg, which is the same as that of ethanol (29100kJ / kg). It can be used as a fuel, and can also be used to prepare polymers, inks, perfumes, antifreeze agents, aromatherapy agents, moisturizers, explosives, and chiral carriers for drugs. It is dehydrated into methyl ethyl ketone, which can be used as a solvent for resins and paints; dehydrated into butadiene, which can be used for synthetic rubber; it can also replace 1,4-butanediol for the synthesis of polyester and polyurethane; Methyl ethyl ketone is condensed and hydrogenated to form octane, which can be used to produce high-grade...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/43
Inventor 沈亚领周文瑜魏东芝孙金杰张燎原杨云龙孙建安胡媛鲍杰
Owner EAST CHINA UNIV OF SCI & TECH
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