Method for extracting oleanolic acid from Kandelia candel leaf

Abstract

The invention provides a method for extracting oleanolic acid from Kandelia leaves, and relates to an oleanolic acid, in particular to a method for extracting purified oleanolic acid from mangrove Kandelia leaves. The invention provides the method for extracting oleanolic acid from Kandelia leaves, which is simple in steps, short in production cycle, high in separation efficiency and high in purity. The method comprises the following steps: Kandelia leaves are washed, dried in the shade and added with methylene chloride for extraction; extract is obtained through filtration and then dried, so as to obtain extractum; the extractum is added to silica gel for chromatography, absorbed and ground into powder; the powder is packed in a column; dient elution is performed by taking petroleum ether / acetone as eluant; 1 to 5 column volumes are rinsed according to that the mass ratio of the petroleum ether to the acetone is 30:1, 10:1 and 4:1 respectively; the powder is washed with methanol, so as to obtain 18 components in all, wherein twelfth to seventeenth components are crude oleanolic acid products; after silica gel column chromatography, and the crude oleanolic acid products are eluted by taking chloroform / methanol as the eluant, and are finally washed with methanol, so as to obtain four components, wherein a third component is just a pure oleanolic acid product.

Description

technical field [0001] The invention relates to oleanolic acid, in particular to a method for extracting and purifying oleanolic acid from mangrove plant candelabra leaves. Background technique [0002] Candel (Kandelia candel) is a mangrove plant growing in tropical and subtropical coasts, belonging to the genus Candel in the family Mangroveaceae. Candela is a shrub or small tree, 2-3m high; with strut roots; leaves leathery, opposite each other; flowers axillary; bark smooth and reddish-brown; branches thick, with enlarged nodes. Candel is mainly distributed in India, Thailand, Vietnam, Malaysia, and the southern part of the Ryukyu Islands in Japan; in China, it is mainly distributed in Guangdong, Guangxi, Fujian, and Taiwan. Folks use candela as medicine, its skin has astringent, hemostatic and antibacterial effects, and the ethanol extract of its root can treat rheumatoid arthritis. Research data show that kangaroo contains active compounds such as polysaccharides, tan...

AI Technical Summary

Problems solved by technology

The plant raw materials for producing oleanolic acid mainly include Ligustrum lucidum, snow gall, green leaf gall, Liaodong wood and Yunnan wood, etc., most of which are wild resources, which are inconvenient to collect and unstable in output, and root bark, stem bark, etc. parts destroy resources

In addition, although the currently generally used purification methods can extract oleanolic acid, there are disadvantages such as complicated process, long time consumption, low extraction rate, low separation efficiency, and low product purity.