Method for extracting oleanolic acid from Kandelia candel leaf

A technology of oleanolic acid and leaves, which is applied in the field of extraction and purification of oleanolic acid, can solve the problems of low separation efficiency, unstable yield, and low product purity, and achieve high separation efficiency, less moldy deterioration, and high purity.

Inactive Publication Date: 2009-06-10
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The plant raw materials for producing oleanolic acid mainly include Ligustrum lucidum, snow gall, green leaf gall, Liaodong wood and Yunnan wood, etc., most of which are wild resources, which are inconvenient to collect and unstable in output, and root bark, stem bark, etc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Fresh candelabra leaves are washed with tap water and dried in a cool and ventilated place. Take 400g, put it in a clean glass jar, add dichloromethane for extraction, the ratio of candelabra leaves:dichloromethane is 1:8. After 24 hours of extraction, the extract was filtered with quantitative filter paper, and dried under reduced pressure at 45° C. to obtain 23.8 g of extract. This extract was subjected to silica gel column chromatography. During the silica gel column chromatography, the same eluate fractions tested by thin layer chromatography were combined into one fraction. First use petroleum ether: acetone as eluent, carry out gradient elution, petroleum ether: acetone is 30: 1, 10: 1 and 4: 1 respectively, wash 3 column volumes in each ratio, wash with methanol finally, obtain altogether 18 components. Wherein the 15th component is the crude product of oleanolic acid, which is then subjected to silica gel column chromatography, using 55:1 chloro...

Embodiment 2

[0018] Embodiment 2: The fresh candelabra leaves are washed with tap water and dried in a cool and ventilated place. Take 400g, put it in a clean glass jar, add dichloromethane for extraction, the ratio of candelabra leaves:dichloromethane is 1:3. After 24 hours of extraction, the extract was filtered with quantitative filter paper, and dried under reduced pressure at 40° C. to obtain 19.4 g of extract. This extract was subjected to silica gel column chromatography. During the silica gel column chromatography, the same eluate fractions tested by thin layer chromatography were combined into one fraction. First use petroleum ether: acetone as eluent, carry out gradient elution, the ratio of petroleum ether: acetone is respectively 30: 1, 10: 1 and 4: 1, wash 3 column volumes in each ratio, wash with methanol at last, A total of 18 components were obtained. Wherein the 14th component is the crude product of oleanolic acid, which is then subjected to silica gel column chromatog...

Embodiment 3

[0019] Embodiment 3: The fresh candelabra leaves are washed with tap water, and dried in a cool and ventilated place. Take 400g, put it in a clean glass jar, add dichloromethane to extract, the ratio of candelabra leaves:dichloromethane is 1:4. After 24 hours of extraction, the extract was filtered with quantitative filter paper, and dried under reduced pressure at 42° C. to obtain 20.3 g of extract. This extract was subjected to silica gel column chromatography. During the silica gel column chromatography, the same eluate fractions tested by thin layer chromatography were combined into one fraction. First use petroleum ether: acetone as eluent, carry out gradient elution, the ratio of petroleum ether: acetone is respectively 30: 1, 10: 1 and 4: 1, wash 3 column volumes in each ratio, wash with methanol at last, A total of 18 components were obtained. Wherein the 17th component is the crude product of oleanolic acid, which is then subjected to silica gel column chromatograp...

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PUM

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Abstract

The invention provides a method for extracting oleanolic acid from Kandelia leaves, and relates to an oleanolic acid, in particular to a method for extracting purified oleanolic acid from mangrove Kandelia leaves. The invention provides the method for extracting oleanolic acid from Kandelia leaves, which is simple in steps, short in production cycle, high in separation efficiency and high in purity. The method comprises the following steps: Kandelia leaves are washed, dried in the shade and added with methylene chloride for extraction; extract is obtained through filtration and then dried, so as to obtain extractum; the extractum is added to silica gel for chromatography, absorbed and ground into powder; the powder is packed in a column; dient elution is performed by taking petroleum ether/acetone as eluant; 1 to 5 column volumes are rinsed according to that the mass ratio of the petroleum ether to the acetone is 30:1, 10:1 and 4:1 respectively; the powder is washed with methanol, so as to obtain 18 components in all, wherein twelfth to seventeenth components are crude oleanolic acid products; after silica gel column chromatography, and the crude oleanolic acid products are eluted by taking chloroform/methanol as the eluant, and are finally washed with methanol, so as to obtain four components, wherein a third component is just a pure oleanolic acid product.

Description

technical field [0001] The invention relates to oleanolic acid, in particular to a method for extracting and purifying oleanolic acid from mangrove plant candelabra leaves. Background technique [0002] Candel (Kandelia candel) is a mangrove plant growing in tropical and subtropical coasts, belonging to the genus Candel in the family Mangroveaceae. Candela is a shrub or small tree, 2-3m high; with strut roots; leaves leathery, opposite each other; flowers axillary; bark smooth and reddish-brown; branches thick, with enlarged nodes. Candel is mainly distributed in India, Thailand, Vietnam, Malaysia, and the southern part of the Ryukyu Islands in Japan; in China, it is mainly distributed in Guangdong, Guangxi, Fujian, and Taiwan. Folks use candela as medicine, its skin has astringent, hemostatic and antibacterial effects, and the ethanol extract of its root can treat rheumatoid arthritis. Research data show that kangaroo contains active compounds such as polysaccharides, tan...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 冯丹青柯才焕卢昌义向成
Owner XIAMEN UNIV
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