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Pumpkin seed oil extraction method using water enzyme method

A technology of pumpkin seed oil and water enzymatic method, which is applied in the direction of edible oil/fat, food science, application, etc., can solve the difference of extraction methods and the conditions of germination (bud length, temperature, humidity are difficult to control, not suitable for large-scale production And other issues

Inactive Publication Date: 2009-06-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The CN1766075A patent emphasizes the extraction of germinated pumpkin seeds. Germination can promote the content of various components in pumpkin seeds, but the germination conditions (bud length, temperature, humidity, etc.) low content of ingredients, not suitable for large-scale production
(2) Differences in extraction methods
The extraction method used in the CN1766075A patent is a conventional enzymatic hydrolysis method, which does not have the advantages of aqueous enzymatic extraction as mentioned above
(3) Orthogonal experiments were not conducted to determine the optimal conditions for conditions such as crushing particle size, substrate concentration, enzyme dosage, compound enzyme ratio, enzymatic hydrolysis pH value, temperature (°C), and time (h).
(4) There are also large differences between the evaluation indicators of pumpkin seed oil

Method used

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  • Pumpkin seed oil extraction method using water enzyme method
  • Pumpkin seed oil extraction method using water enzyme method
  • Pumpkin seed oil extraction method using water enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0021] Embodiment 1: Determination of the optimal enzymatic hydrolysis conditions for the aqueous enzymatic method

[0022] Determine the pumpkin seed size of pumpkin seed oil produced by aqueous enzymatic method:

[0023] Use a high-speed universal cell grinder to crush the pumpkin seeds for 5 seconds (note that the degree of crushing is not too high), and use a standard inspection sieve to screen out pumpkin seed powders with particle sizes of 0.325 mm, 0.5 mm, 1.0 mm, and 1.5 mm. Take 7g of raw materials with the above particle size respectively, and add 0.1mol / L acetic acid-sodium acetate buffer solution with a pH value of 4.8 at a solid-to-liquid ratio of 1:2. Add 0.5mL cellulase stock solution, enzymatically hydrolyze at 50°C for 2h, centrifuge, add 56mL n-hexane solvent to extract, and rotate to evaporate the oil sample. After constant weight, use the oil yield as an indicator to determine the optimum crushing particle size for pumpkin seed oil extraction Control withi...

Embodiment approach 2

[0046] Embodiment 2: Extraction of pumpkin seed oil by aqueous phase enzymatic hydrolysis and organic solvent extraction

[0047] Use a high-speed universal cell grinder to crush the pumpkin seeds for 5 seconds (note that the degree of crushing should not be too high), take 7 g of pumpkin seed powder that has passed through a 0.5 mm aperture sieve, add 25500 U of compound enzyme, and then add 0.1 mol / L acetic acid-sodium acetate buffer solution to 21mL, enzymolysis at pH 4, 40°C for 4h, high-speed centrifugation (3000-10000r / min) of the solution obtained after enzymolysis to break the emulsion, adding 56mL of n-hexane solvent to the centrifugation liquid, extracting 3-4 times, and combining the extraction The oil sample was obtained by rotary evaporation, and the pumpkin seed oil yield was 38.34% after constant weight.

[0048] To test the residual determination of n-hexane in the aqueous phase enzymatic hydrolysis organic solvent oil extraction method.

[0049] 20g of pumpki...

Embodiment approach 3

[0053] Embodiment 3: Extraction of pumpkin seed oil by aqueous phase enzymatic hydrolysis

[0054] Use a high-speed universal cell grinder to crush the pumpkin seeds for 5 seconds (note that the degree of crushing should not be too high), take 7 g of pumpkin seed powder that has passed through a 0.5 mm aperture sieve, add 25500 U of compound enzyme, and then add 0.1 mol / L acetic acid-sodium acetate buffer solution to 21mL, enzymolysis at pH 4, 40°C for 4h, high-speed centrifugation (3000-10000r / min) of the solution obtained after enzymolysis to break the emulsion, adding aqueous solution to the centrifuge to extract oil, adding demulsifier saturated NaCl solution 5-15mL , extracted 3 to 4 times, and added 0.1 to 0.3 g of anhydrous CaCl to the obtained oil phase 2 After drying and centrifuging, a clear, yellow-green oil is obtained, and the oil yield of pumpkin seeds is 37.40%. 100g of pretreated pumpkin seeds were weighed, and the scale-up experiment was carried out according...

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Abstract

A method for extracting pumpkin seed oil using a water-enzyme method pertains to the vegetable oil extraction technology, including: using pumpkin seed powders with the particle diameter between 0.3mm to 1.5mm after screening, performing buffering liquid enzymolysis under the enzymolysis condition of 15000-30000U of complex enzyme dosage, 1 to 7 of the solution pH value, 2-8h of extraction time, and 20-60 DEG C of extraction temperature; after demulsification by high-speed centrifugation, adding a water solution into the centrifugal liquid, and then adding a demulsifying agent, using a separating funnel for demixing, adding a desiccant, and then obtaining lipid after centrifugation; or adding an organic solvent in the centrifugate, rotarily evaporating the solvent to obtain lipid. The invention has advantages of high oil yield, good lipid quality, simple process equipment, low consumption, less machining programs, and capability of being easy for the industrial production use.

Description

technical field [0001] The invention belongs to vegetable oil extraction technology, and mainly relates to a pumpkin seed oil extraction method. Background technique [0002] Pumpkins are planted in a wide range in my country, and pumpkin seeds are rich in sources. However, only a small part of pumpkin seeds are used to make dried fruit roasted seeds and health drinks, and most of them are exported to Eastern Europe, Japan and other regions. The resource development and utilization are insufficient, and the extraction and utilization technology of pumpkin seed oil has always been a difficult problem restricting the industrial development of pumpkin seeds. Pumpkin seed oil is rich in unsaturated fatty acids, natural vitamins, phytosterols, linoleic acid and other highly bioactive components, among which the content of unsaturated fatty acids is relatively high, with an average content of 16.59% of oleic acid and 39.53% of linoleic acid. The use of pumpkin seed oil can effect...

Claims

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Application Information

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IPC IPC(8): A23D9/02
Inventor 徐雅琴孙欣杨昱
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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