Pumpkin seed oil extraction method using water enzyme method

A technology of pumpkin seed oil and water enzymatic method, which is applied in the direction of edible oil/fat, food science, application, etc., can solve the difference of extraction methods and the conditions of germination (bud length, temperature, humidity are difficult to control, not suitable for large-scale production And other issues

Inactive Publication Date: 2009-06-17
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The CN1766075A patent emphasizes the extraction of germinated pumpkin seeds. Germination can promote the content of various components in pumpkin seeds, but the germination conditions (bud length, temperature, humidity, etc.) low content of ingredients, not suitable for large-scale production
(2) Differences in extraction methods
The extraction method used in the CN1766075A patent is a conventional enzymatic hydrolysis method,

Method used

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  • Pumpkin seed oil extraction method using water enzyme method
  • Pumpkin seed oil extraction method using water enzyme method
  • Pumpkin seed oil extraction method using water enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Embodiment 1: Determination of the optimal conditions for hydrolysis

[0022] Determine the pumpkin seed particle size for preparing pumpkin seed oil by aqua-enzymatic method:

[0023] Use a high-speed universal cell crusher to crush pumpkin seeds for 5 seconds (note that the crushing degree should not be too high), and use a standard inspection sieve to screen out pumpkin seed powder with particle sizes of 0.325mm, 0.5mm, 1.0mm, and 1.5mm. Separately take 7 g of the raw materials with the above particle size, and add a 0.1 mol / L acetic acid-sodium acetate buffer solution with pH 4.8 at a solid-to-liquid ratio of 1:2. Add 0.5 mL of cellulase stock solution and enzymatically digest at 50°C for 2 hours. After centrifugation, add 56 mL of n-hexane solvent to extract and spin-evaporate the oil sample. After constant weight, the oil yield is used as an indicator to determine the optimal crushing particle size of pumpkin seed oil. Control within 0.3~1.5mm.

[0024] Determine the ...

Example Embodiment

[0046] Embodiment 2: Extraction of pumpkin seed oil by water-phase enzymatic hydrolysis and organic solvent extraction method

[0047] Use a high-speed universal cell pulverizer to crush pumpkin seeds for 5 seconds (note that the degree of crushing is not too high), take 7g of pumpkin seed powder that has passed through a 0.5mm aperture sieve, add 25500U compound enzyme, and then add 0.1mol / L acetic acid-sodium acetate buffer solution to 21mL, enzymatically hydrolyze at pH 4, 40℃ for 4h, centrifuge the solution obtained after enzymatic hydrolysis at high speed (3000~10000r / min) to break the emulsion, add 56mL of n-hexane solvent to the centrifugal liquid, extract 3~4 times, and combine extraction The oil sample was produced by rotary evaporation, and the oil yield of pumpkin seeds after constant weight was 38.34%.

[0048] Test the residual determination of n-hexane in the method of extracting oil from organic solvents by enzymatic hydrolysis in the aqueous phase.

[0049] After 2...

Example Embodiment

[0053] Embodiment 3: Extraction of pumpkin seed oil by water-phase enzymatic hydrolysis

[0054] Use a high-speed universal cell pulverizer to crush pumpkin seeds for 5 seconds (note that the degree of crushing is not too high), take 7g of pumpkin seed powder that has passed through a 0.5mm aperture sieve, add 25500U compound enzyme, and then add 0.1mol / L acetic acid-sodium acetate buffer solution to 21mL, enzymatically hydrolyze at pH 4, 40℃ for 4h, centrifuge the solution obtained after enzymatic hydrolysis at high speed (3000~10000r / min) to demulsify, add aqueous solution to the centrifugal liquid to extract oil, add 5~15mL of saturated NaCl solution of demulsifier , Extract 3~4 times, add 0.1~0.3g anhydrous CaCl to the obtained oil phase 2 After drying and centrifugation, clear, yellow-green oil is obtained, and the oil yield of pumpkin seeds is 37.40%. Weigh 100 g of the pretreated pumpkin seeds, and carry out an expanded experiment according to the above-mentioned water-phas...

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Abstract

A method for extracting pumpkin seed oil using a water-enzyme method pertains to the vegetable oil extraction technology, including: using pumpkin seed powders with the particle diameter between 0.3mm to 1.5mm after screening, performing buffering liquid enzymolysis under the enzymolysis condition of 15000-30000U of complex enzyme dosage, 1 to 7 of the solution pH value, 2-8h of extraction time, and 20-60 DEG C of extraction temperature; after demulsification by high-speed centrifugation, adding a water solution into the centrifugal liquid, and then adding a demulsifying agent, using a separating funnel for demixing, adding a desiccant, and then obtaining lipid after centrifugation; or adding an organic solvent in the centrifugate, rotarily evaporating the solvent to obtain lipid. The invention has advantages of high oil yield, good lipid quality, simple process equipment, low consumption, less machining programs, and capability of being easy for the industrial production use.

Description

technical field [0001] The invention belongs to vegetable oil extraction technology, and mainly relates to a pumpkin seed oil extraction method. Background technique [0002] Pumpkins are planted in a wide range in my country, and pumpkin seeds are rich in sources. However, only a small part of pumpkin seeds are used to make dried fruit roasted seeds and health drinks, and most of them are exported to Eastern Europe, Japan and other regions. The resource development and utilization are insufficient, and the extraction and utilization technology of pumpkin seed oil has always been a difficult problem restricting the industrial development of pumpkin seeds. Pumpkin seed oil is rich in unsaturated fatty acids, natural vitamins, phytosterols, linoleic acid and other highly bioactive components, among which the content of unsaturated fatty acids is relatively high, with an average content of 16.59% of oleic acid and 39.53% of linoleic acid. The use of pumpkin seed oil can effect...

Claims

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Application Information

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IPC IPC(8): A23D9/02
Inventor 徐雅琴孙欣杨昱
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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