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Method for reproducing waste rubber by using microorganism

A technology for regenerating waste rubber and microorganisms, applied in the field of waste rubber regeneration, can solve the problems of rubber main chain bond oxidation, serious secondary pollution, and low production efficiency, and achieve the effects of mild conditions, low equipment requirements, and short growth cycle

Inactive Publication Date: 2011-07-20
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The disadvantages of the traditional desulfurization method are: ① In addition to cutting off the sulfur bond cross-linking point, it will also cause oxidation and partial thermal cracking of the rubber main chain bond
②Serious secondary pollution, low production efficiency and high energy consumption

Method used

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  • Method for reproducing waste rubber by using microorganism
  • Method for reproducing waste rubber by using microorganism
  • Method for reproducing waste rubber by using microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Materials

[0050] 1 strain: Saccharomyces cerevisiae.

[0051] 2 Natural rubber powder: Yunbiao 1# Yunnan Natural Rubber Industry Co., Ltd.

[0052] 3 media

[0053](1) Slant medium: glucose 20g / L, yeast extract powder 10g / L, peptone 10g / L, agar 15g / L; pH 5.0-6.0, sterilized at 115°C, 0.1Mpa for 30min;

[0054] (2) Seed medium: glucose 50g / L, yeast extract powder 10g / L, peptone 10g / L, malt extract powder 5g / L; pH 5.0-6.0, 115°C, 0.1Mpa sterilization for 30min;

[0055] (3) Desulfurization medium: glucose 40g / L, yeast extract powder 15g / L, peptone 15g / L, malt extract powder 5g / L, MgSO 4 ·7H 2 O5g / L, K 2 HPO 4 ·3H 2 O 1g / L, KH 2 PO 4 1g / L, ZnSO 4 0.044g / L, FeSO 4 ·7H 2 O0.03g / L, CuCl 2 0.016g / L, MnSO 4 0.019g / L; pH 5.0, 115°C, 0.1Mpa sterilization for 20min.

[0056] 4 Instruments and equipment: shaking incubator, reactor.

[0057] 2. Method

[0058] 1 Culture of seed solution

[0059] (1) Primary seed liquid: take Saccharomyces cerevisiae cells pr...

Embodiment 2

[0065] 1. Materials

[0066] 1 strain: Saccharomyces cerevisiae.

[0067] 2 Natural rubber powder: Yunbiao 1# Yunnan Natural Rubber Industry Co., Ltd.

[0068] 3 media:

[0069] (1) Slant medium: glucose 20g / L, yeast extract powder 10g / L, peptone 10g / L, agar 15g / L; pH 5.0-6.0, 115°C, 0.1Mpa sterilization for 30min;

[0070] (2) Seed medium: glucose 50g / L, yeast extract powder 10g / L, peptone 10g / L, malt extract powder 5g / L; pH 5.0-6.0, 115°C, 0.1Mpa sterilization for 30min;

[0071] (3) Desulfurization medium: glucose 40g / L, yeast extract powder 15g / L, peptone 15g / L, malt extract powder 5g / L, MgSO 4 ·7H 2 O5g / L, K 2 HPO 4 ·3H 2 O 1g / L, KH 2 PO 4 1g / L, ZnSO 4 0.044g / L, FeSO 4 ·7H 2 O0.03g / L, CuCl 2 0.016g / L, MnSO 4 0.019g / L; pH5.5, 115℃, 0.1Mpa sterilization for 20min.

[0072] 4 Instruments and equipment: shaking incubator, reactor.

[0073] 2. Method

[0074] 1 Culture of seed solution

[0075] (1) Primary seed liquid: take Saccharomyces cerevisiae cells pres...

Embodiment 3

[0081] 1. Materials

[0082] 1 strain: Saccharomyces cerevisiae.

[0083] 2 Natural rubber powder: Yunbiao 1# Yunnan Natural Rubber Industry Co., Ltd.

[0084] 3 media

[0085] (1) Slant medium: glucose 20g / L, yeast extract powder 10g / L, peptone 10g / L, agar 15g / L; pH 5.0-6.0, 115°C, 0.1Mpa sterilization for 30min;

[0086] (2) Seed medium: glucose 50g / L, yeast extract powder 10g / L, peptone 10g / L, malt extract powder 5g / L; pH 5.0-6.0, 115°C, 0.1Mpa sterilization for 30min;

[0087] (3) Desulfurization medium: glucose 40g / L, yeast extract powder 15g / L, peptone 15g / L, malt extract powder 5g / L, MgSO 4 ·7H 2 O5g / L, K 2 HPO 4 ·3H 2 O 1g / L, KH 2 PO 4 1g / L, ZnSO 4 0.044g / L, FeSO 4 ·7H 2 O0.03g / L, CuCl 2 0.016g / L, MnSO 4 0.019g / L; pH5.0-6.0, 115℃, 0.1Mpa sterilization for 20min.

[0088] 4 Instruments and equipment: shaking incubator, reactor.

[0089] 2. Method

[0090] 1 Culture of seed solution

[0091] (1) Primary seed liquid: take Saccharomyces cerevisiae cells p...

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Abstract

The invention provides a method for using microorganism to regenerate scrap rubber. The microorganism used is saccharomyces cerevisiae. Enzyme and metabolin containing hydrosulphonyl produced in the process of growth of cells of saccharomyces cerevisiae reacts with the scrap rubber, and sulfur cross bond of the rubber is fractured and decoking regeneration is carried on, thus reaching the aim of targeted decoking. The decoking is carried on by using co-cultivating the rubber powder and saccharomyces cerevisiae germs and the scrap rubber decoking regeneration effects are strengthened by combining thermal cracking broken wall reaction of the saccharomyces cerevisiae cells, a phase transfer agent and an addition agent of the phase transfer agent. The excellent technical conditions of the decoking regeneration are confirmed: temperature, pH, rotation speed, reaction time, doze of the phase transfer agent and the addition agent of the phase transfer agent, etc. By checking the cross bond density and the degree of swelling and showing the detection of X ray electron spectrum (XPS), after the function of saccharomyces cerevisiae, the cross bond density of the decoking regeneration rubberis obviously reduced, and the degree of swelling is obviously improved, sulfur element contents on the regeneration rubber surface is decreased, thus showing that the decoking regeneration effects ofthe saccharomyces cerevisiae is applied to the rubber surface.

Description

technical field [0001] The invention relates to waste rubber regeneration technology, in particular to a method and process for using yeast to carry out targeted desulfurization of waste rubber, that is, sulfur bond breaking and desulfurization regeneration. Background technique [0002] At present, the world produces more than 10 million tons of waste rubber every year. Waste rubber has a stable three-dimensional chemical network structure that neither melts nor dissolves. The disposal of waste rubber has always been a difficult problem in the fields of environmental protection and rubber reuse. The recycling of waste rubber is conducive to the full utilization of resources and environmental protection, and is an inevitable requirement for sustainable development. [0003] Since the birth of the rubber industry in 1839, the problem of waste rubber disposal has been objectively placed in front of people. The recycling of waste rubber has always attracted much attention. S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J11/10C08L21/00C12P1/02C12R1/865
CPCY02W30/62
Inventor 王雅琴赵素合张翠茹吕杰姜广明霍瑞明
Owner BEIJING UNIV OF CHEM TECH
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