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Natural high-purity nano hydroxylapatite leaching technology

A technology of nano-hydroxyapatite and hydroxyapatite, which is applied in the field of preparation of biomedical materials and can solve problems such as loss

Inactive Publication Date: 2009-06-24
孙炜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method basically completely destroys the crystal structure of hydroxyapatite, and the obtained crystalline material completely loses the natural trabecular bone scaffolding structure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Select fresh 1-year-old bovine femur, remove the soft tissue, and process the cancellous bone at both ends of the femur and the cortical bone of the femoral shaft separately. The cortical bone was cut into 1cm×1cm size bone pieces, and the cancellous bone was cut into 1.5cm×1.5cm size bone pieces. In a high-pressure tank, boil for 2 hours at 100°C under a pressure of 5-10kg to carry out preliminary aging and degreasing. Dry in a drying oven at 60-80°C for 8-10 hours, and place at room temperature for 24 hours.

[0032] Put the pre-treated material into a Soxhlet extractor, add toluene and extract for 24 hours. Separate and recover the material residue, rinse with running water, dry at room temperature, and soak in ethanol for 24 hours to remove the organic solvent.

[0033] Take 2000g of defatted bone, put it into a Soxhlet extractor, add 1000ml of 99% ethylenediamine and 150ml of deionized water. Repeat to mix. Heating and azeotropic extraction, the azeotropic mixt...

Embodiment 2

[0037] The finished granules in Example 1 were tested. Testing includes X-ray diffraction analysis, scanning electron microscopy, purity determination, etc.

[0038] According to X-ray diffraction analysis of the sample, the main component is relatively pure hydroxyapatite. Under the scanning electron microscope, it can be observed that the crystal structure of hydroxyapatite is irregular and flaky, with a thickness of 5-10nm and a length of 10-50nm, which is completely consistent with the crystal structure of hydroxyapatite of fresh bone. In addition, no organic matter remains under the electron microscope .

[0039] Purity determination: determined by total nitrogen and hydroxyproline analysis. Protein content <0.06%, collagen content <0.05%.

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PUM

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Abstract

The invention relates to a process for leaching a natural high-purity nanometer hydroxyapatite biomaterial. The hydroxyapatite keeps the basic crystal structure not damaged, and is a base biomaterial, the performance of the base biomaterial is closer to the biological performance of a bone, and the crystal structure is more suitable for the growth of bone cells and osteoclasts. The leaching process adopts a physical leaching method and a chemical leaching method which are alternately applied; and by the pure solute dissolution flow and chemical reactions under specific conditions and in specific solvents, organic compositions in the bone are dissolved, and the used solvents include water, ethanol, ketone or other organic solvents such as amine and the like. In the leaching process, the control temperature is adjusted, and the boiling water is repeatedly washed so as to ensure the purity and the nanometer crystal property of the natural hydroxyapatite.

Description

technical field [0001] The invention relates to a leaching process of a natural high-purity nano-hydroxyapatite biological material, which belongs to the field of biomedical material preparation. Background technique [0002] In the bones of mammals such as cattle, pigs, and sheep, the content of hydroxyapatite reaches 50-60%. This natural hydroxyapatite occurs in living organisms as a very fine crystal structure. This crystal structure form is a weakly crystallized, irregular, sheet-like form with a thickness of 5-10nm and a length of 10-50nm, which is a natural nanostructure. Its calcium content is 37.5% to 38.0%, and its phosphorus content is 15.5 to 19.0%. This suitable calcium / phosphorus ratio determines the good chemical properties of its surface. This crystallization method is the material basis for the good performance of tissues and organs, and it is difficult to be replicated by chemical synthesis methods in vitro. Therefore, if high-purity natural hydroxyapatite...

Claims

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Application Information

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IPC IPC(8): C01B25/32
Inventor 孙炜
Owner 孙炜
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