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Novel technique for producing heparin

A production process, a new process technology, applied in the new process field of preparing heparin by biological enzymatic hydrolysis, can solve the problems of waste water pollution, incomplete hydrolysis, long production cycle, etc., and achieve economic benefits, maximum benefits, and less impurities Effect

Inactive Publication Date: 2009-06-24
DAYING MAOSEN BIOCHEM FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this production process has a long production cycle, incomplete and incomplete hydrolysis, and the heparin produced has low purity, low yield, and low potency, and produces a large amount of waste residue and waste water that seriously pollutes the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Production technology of the present invention comprises the following steps:

[0045] 1) Preparation of fresh pancreatic juice

[0046] ① First prepare quicklime water, mix quicklime and water in a ratio of 1:3, stir well and settle for later use;

[0047] ②Remove the peduncle tissue, grease and sundries from the fresh pancreas, and grind it into a paste;

[0048] ③ Mix the precipitated quicklime supernatant with the crushed pancreas paste in a ratio of 1:2-3 to form pancreas pulp;

[0049] ④Use 30-40% sodium hydroxide solution to adjust the pH value to 8.0, stir well and let it stand for 2 hours. After activation, it can be used;

[0050] 2) Enzymolysis

[0051] ①Put 100kg of the collected intestinal mucosa into the reaction pot, add 0.2kg of phenol solution, and add 700kg of water to prepare the enzymolysis solution;

[0052] ② When heating to 45°C, use 45% sodium hydroxide solution to adjust the pH value to 8.5, add 20kg of fresh pancreas juice, continue to heat...

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PUM

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Abstract

The invention provides a new heparin production process. The process comprises the following steps: firstly, fresh pancreatic serum is produced, and then pig intestinal mucosa is subject to biological enzymolysis to obtain solution containing the heparin, the solution passes through D254 ion exchange resin for adsorption and elution, and a crude heparin product is obtained by the steps of precipitation, desalination, dehydration, drying and the like. The process is characterized by short production period, low consumption of raw materials and auxiliary materials, low energy consumption, high purity, high potency and no environmental pollution, and the yield of the product can reach 0.8-1g when the potency is 75-85U.

Description

technical field [0001] The invention relates to a heparin production process, in particular to a new process for preparing heparin by a biological enzymatic hydrolysis method. Background technique [0002] As a biomedical intermediate, heparin is a natural anticoagulant drug that has anticoagulant and antithrombotic effects. Preservation of blood and so on. Heparin is widely distributed in mammalian tissues, such as intestinal mucosa, lung, liver, especially in pig small intestinal mucosa with the highest content. The traditional heparin production process is the salt solution method, that is, primary biochemical reaction occurs between alkaline saline and intestinal mucosa to produce heparin. However, this production process has a long production cycle, incomplete and incomplete hydrolysis, and the heparin produced has low purity, low yield, and low potency, and produces a large amount of waste residue and waste water that seriously pollutes the environment. Contents of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
Inventor 秦平
Owner DAYING MAOSEN BIOCHEM FACTORY