Method for separating and extracting amber acid from amber acid fermentation liquor by nanofiltration

A technology of succinic acid and fermentation broth, applied in the separation/purification of carboxylic acid compounds, ultrafiltration, carboxylate preparation and other directions, can solve the problems of difficult product quality control, large sewage, and many steps, and save equipment investment. , The effect of good separation selectivity and low cost of raw materials

Inactive Publication Date: 2009-07-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a method for separating and extracting succinic acid from succinic acid fermentation liquid using nanofiltration

Method used

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  • Method for separating and extracting amber acid from amber acid fermentation liquor by nanofiltration

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The succinic acid fermentation broth prepared with glucose as carbon source contains 67g / L succinic acid, 12g / L acetic acid, 6g / L formic acid, 9g / L glucose, pH6.7.

[0027] Anionic flocculants were added to the fermentation broth, and diatomaceous earth was used as a filter aid for plate-and-frame pressure filtration. The filtration temperature was 70°C, the sterilization rate was 91%, and the protein removal rate was 27%. The filtrate was collected; the filtrate was adjusted to pH5 with sulfuric acid .0, using a tubular inorganic membrane with a molecular weight cut-off of 150,000 Daltons for ultrafiltration, temperature 35°C, operating pressure 0.3Mpa, protein removal rate 75%, ultrafiltrate collection, decolorization rate 27%; two nanofiltration processes The molecular weight cut-off of the nanofiltration membrane adopted is 150 Daltons, the ultrafiltrate is adjusted to pH 3.0 with sulfuric acid, and the nanofiltration separation is carried out, the temperature is 25 ...

Embodiment 2

[0030]The succinic acid fermentation broth prepared with glucose as carbon source contains succinic acid 45g / L, acetic acid 9g / L, formic acid 5g / L, glucose-free, pH 6.5.

[0031] Use sulfuric acid to adjust the fermentation broth to pH 4.5 for microfiltration. The pore size of the tubular inorganic microfiltration membrane used is 0.175 μm, the operating temperature is 40 ° C, the operating pressure is 0.1 Mpa, the sterilization rate is 99.8%, and the protein removal rate is 41%. , collect the filtrate; adjust the filtrate to pH 4.5 with sulfuric acid, use a roll-type organic membrane with a molecular weight cut-off of 10,000 Daltons for ultrafiltration, the temperature is 25°C, the operating pressure is 0.25Mpa, and the protein removal rate is 90%. liquid, the decolorization rate is 68%; the molecular weight cut-off of the nanofiltration membrane used in the two nanofiltration processes is 160 Daltons, and the ultrafiltrate is adjusted to pH4. Mpa, succinic acid transmission ...

Embodiment 3

[0034] Succinic acid fermentation broth prepared by using straw hydrolyzed sugar solution as carbon source, containing 51g / L succinic acid, 11g / L acetic acid, 7g / L formic acid, no glucose, pH6.4,

[0035] Use sulfuric acid to adjust the fermented liquid to pH 4.0 for microfiltration. The hollow fiber organic microfiltration membrane used has a pore size of 0.1 μm, an operating temperature of 20° C., a sterilization rate of 99.5%, and a protein removal rate of 35%. The filtrate is collected; Sulfuric acid was used to adjust the filtrate to pH 4.0, and ultrafiltration was carried out with a roll-type organic membrane with a molecular weight cut-off of 5000 Daltons. The temperature was 40°C, the operating pressure was 0.3Mpa, the protein removal rate was 95%, and the ultrafiltrate was collected with a decolorization rate of 80%; The molecular weight cut-off of the nanofiltration membrane used in the two nanofiltration processes is 160 Daltons, the ultrafiltrate is adjusted to pH 3...

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Abstract

The invention discloses a method for separating and extracting butane diacid by nano-filtration from a butane diacid zymotic fluid. The method comprises the following steps that: the butane diacid zymotic fluid is subjected to prefiltration, and hyperfiltration under an acidity condition; an hyperfiltration permeate liquid is subjected to nano-filtration under an acidity condition, a nano-filtration permeate liquid is subjected to re-nano-filtration under a neutrality condition; a nano-filtration trapped fluid is subjected to reduced pressure evaporation concentration; and a concentrated solution is subjected to cooling crystallization under an alkalinity condition to prepare succinic acid disodium salt or under an acidity condition to prepare the butane diacid. The method does not use any organic solvent or active carbon in the processes of separation and extraction of the butane diacid or the succinic acid disodium salt, and completes decolorization, purification, desalinization, preconcentration and other processes of the butane diacid in the same nano-filtration separation device. The method has the advantages of mild condition, convenient operation, good selectivity, low cost of raw materials, high product quality, small pollution, and the like, can recover unconsumed substrate of the zymotic fluid, and has wide social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of biological separation engineering, and in particular relates to a method for separating and extracting succinic acid from succinic acid fermentation liquid by using nanofiltration technology. Background technique [0002] Succinic acid (Succinic Acid, Butanedioic Acid), also known as succinic acid, is an important dibasic organic carboxylic acid and an important metabolite of microbial tricarboxylic acid cycle and anaerobic fermentation. It is widely used in degradable materials, pharmaceuticals, Surfactants, protective coatings, dyes and other industries. And its salt—disodium succinate (scallop) is also an important food additive, which has a special seafood flavor and is widely used in the food industry. The production of succinic acid from butane through maleic anhydride has limited the wide application of succinic acid as a basic chemical raw material due to the cost of raw materials and environment...

Claims

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Application Information

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IPC IPC(8): C07C55/10C07C51/42C07C51/41B01D61/14
Inventor 吴昊姜岷韦萍雷丹姚忠左鹏
Owner NANJING UNIV OF TECH
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