Induced extracting method for pigeon pea leaf extract rich in flavone activity component

A technology of active ingredients and extraction methods, which is applied in the field of induced extraction of pigeonpea leaf extract rich in flavonoid active ingredients, to achieve the effect of improving free, low energy consumption, and increasing content

Inactive Publication Date: 2009-07-22
NORTHEAST FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are many reports on enzyme pretreatment to extract oil and protein and negative pressure cavitation extraction of compounds, but the combination of enzyme induction technology and negative pressure cavitation extraction technology to extract active ingredients in pigeonpea leaves has not been reported

Method used

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  • Induced extracting method for pigeon pea leaf extract rich in flavone activity component
  • Induced extracting method for pigeon pea leaf extract rich in flavone activity component
  • Induced extracting method for pigeon pea leaf extract rich in flavone activity component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Prepare an enzyme solution with a concentration of β-glucosidase of 0.5 mg / mL and a concentration of pectinase of 0.4 mg / mL, and adjust the pH between 3.6 and 4.0. Weigh 20g of fresh pigeonpea leaves, add enzyme solution at a solid-to-liquid ratio of 1:8, homogenize for 2 minutes, put the homogenate solution in a constant temperature shaker at 35°C for 36 hours, and then filter it with suction to remove the solid-liquid Add 70% ethanol solution at a ratio of 1:10 and extract by negative pressure cavitation for 3 times, 80 min each time, combine the extracts, concentrate, extract with petroleum ether and ethyl acetate respectively, combine the ethyl acetate layers, concentrate to dryness, and obtain a solid 0.75g, constant volume with methanol, LC-MS / MS detection, the contents of vitexin, isovitexin, luteolin, apigenin and isorhamnetin in the obtained solid were 0.246, 0.153, 0.213, 0.117, 0.083mg / g, respectively increased by 27.5%, 14.2%, 30.6%, 28.6% and 33.9% compared...

Embodiment 2

[0035] Prepare a cellulase solution with a concentration of 0.3 mg / mL and adjust the pH between 5.0 and 6.0. Weigh 20g of fresh pigeonpea leaves, add enzyme solution at a solid-to-liquid ratio of 1:5, homogenate for 1min, put the homogenate solution in a constant temperature shaker at 45°C for 24h, and filter with suction to dissolve the solid-liquid Add 50% ethanol solution at a ratio of 1:10 for negative pressure cavitation extraction twice, each time for 85 minutes, combine the extracts, concentrate, extract with petroleum ether and ethyl acetate respectively, combine the ethyl acetate layers, concentrate to dryness, and obtain a solid 0.76g, constant volume with methanol, LC-MS / MS detection, the contents of vitexin, isovitexin, luteolin, apigenin and isorhamnetin in the obtained solid were 0.223, 0.145, 0.194, 0.108, 0.074mg / g, respectively increased by 15.5%, 8.2%, 19.1%, 18.7% and 19.3% compared with the traditional heat reflux method.

Embodiment 3

[0037] Prepare an enzyme solution with a cellulase concentration of 0.5 mg / mL and a β-glucosidase concentration of 0.5 mg / mL, and adjust the pH between 4.5 and 5.5. Weigh 20g of fresh pigeonpea leaves, add enzyme solution at a solid-to-liquid ratio of 1:8, homogenate for 1.5min, put the homogenate solution in a constant temperature shaker at 30°C for 18h, and filter it with suction to remove the solid Liquid ratio 1:15, add ethanol negative pressure cavitation extraction 3 times, 70min each time, combine the extracts, concentrate, extract with petroleum ether and ethyl acetate respectively, combine the ethyl acetate layers, concentrate to dryness, and obtain 0.8g of solid matter , constant volume with methanol, LC-MS / MS detection, the contents of vitexin, isovitexin, luteolin, apigenin and isorhamnetin in the obtained solid were 0.296, 0.187, 0.264, 0.150, 0.102mg respectively / g, respectively increased by 53.4%, 39.6%, 62.0%, 64.8% and 64.5% compared with the traditional heat...

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Abstract

The invention relates to the extraction technology of active ingredients of cajan leaf which is a Chinese herbal medicine, and aims at providing a high-efficiency enzyme inductive extraction method which can economically and quickly obtain a cajan leaf extract rich in five flavonoid active ingredients, namely agnuside, isovitexin, luteolin, apigenin and isorhamnetin. The method is directed against the shortcomings of the traditional extraction method such as long extraction time, high extraction temperature, low extraction efficiency and the like and adopts technologies such as homogenate fragmentation enzymolysis, homothermal enzymolysis, vacuum cavitation extraction, solvent decontamination and enrichment and the like to obtain the cajan leaf extract rich in five flavonoid active ingredients; and the result shows that: the content of the five flavonoid active ingredients contained in the extract can respectively reach 0.223 to 0.296, 0.145 to 0.187, 0.194 to 0.264, 0.108 to 0.150 and 0.074 to 0.102 mg / g, which is 9 percent to 65 percent higher than the content of the five flavonoid active ingredients contained in the extract obtained by the traditional hot reflux method. The method is characterized by high extraction ratio, simple equipment and safe operation, needs no high-temperature heating, is applicable to industrial production and provides a new method and means for the modernization of Chinese traditional medical extraction.

Description

technical field [0001] The invention relates to a method for efficiently inducing and increasing the content of active ingredients in Chinese herbal medicines and extracting them efficiently, that is, obtaining pigeonpea leaves rich in vitexin, isovitexin, luteolin, apigenin and isorhamnetin Enzyme Induction-Negative Pressure Cavitation Extraction Method for Extracts. Background technique [0002] Pigeon pea [Cajanus cajan (L.) Millsp.], commonly known as pigeonpea in English, is an annual or perennial tropical or subtropical woody plant, evergreen shrub. Also known as pigeon bean, bean paste tree, tree bean, etc., it is a kind of folk medicine. Pigeon leaf has the effects of antibacterial and anti-inflammatory, detoxification and swelling, promoting blood circulation and removing blood stasis, stopping bleeding and relieving pain, nourishing kidney and strengthening bones, removing putrefaction and promoting muscle growth, and anti-infection. . External treatment of chil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07D311/30C12P17/06A61P39/06A61P35/00A61P31/00A61P29/00A61P11/10A61P11/06A61P1/04A61P1/16A61P7/10A61P9/12A61P3/06A61P9/10A61P7/02
Inventor 付玉杰祖元刚刘威佟美鸿孔羽杨梅王芳
Owner NORTHEAST FORESTRY UNIVERSITY
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