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Mercury resistant chelate monoclonal antibody, as well as preparation method and application thereof

A monoclonal antibody and chelate technology, applied in chemical instruments and methods, instruments, analytical materials, etc., can solve the problems of inability to use on-site detection, limited processing capacity, long detection time, etc., and achieve fast detection speed and selectivity Strong, high titer effect

Inactive Publication Date: 2009-07-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional heavy metal detection methods mostly use chemical instruments, such as atomic absorption spectroscopy (atomic absorption spectroscopy, AAS), inductively coupled plasma atomic emission spectroscopy (ICP-AES), etc. processing, and the detection process must be carried out indoors with centralized large-scale analytical instruments, which cannot be used for on-site detection, and has defects such as high cost, limited processing capacity, and long detection time

Method used

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  • Mercury resistant chelate monoclonal antibody, as well as preparation method and application thereof
  • Mercury resistant chelate monoclonal antibody, as well as preparation method and application thereof
  • Mercury resistant chelate monoclonal antibody, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Dissolve BSA (bovine serum albumin) and KLH (keyhole limpet hemocyanin) in PBS (phosphate buffered saline) at pH 9.5, respectively, with a concentration of 4 mg / mL;

[0040] (2) 10 mg of chelating agent ITCBE was dissolved in 0.1 mol / L, pH 9.5 sodium phosphate buffer solution, the concentration was 18 mg / mL;

[0041] (3) Synthesis of hapten Hg-ITCBE: 1000μL 1mg / mLHg 2+Add the standard solution to 2000 μL of 4.6mM chelating agent solution, adjust the pH to 7.0 with ammonia water, and stir for 12 hours at 20°C;

[0042] (4) Synthesis of immunogen Hg-ITCBE-KLH: take 2 mg of hapten, add 2 mg of KLH, adjust the pH to 8.5 with ammonia water, and react with stirring at 20° C. for 12 hours.

[0043] (5) Synthesis of coated antigen Hg-ITCBE-BSA: take 2 mg of hapten, add 2 mg of BSA, adjust the pH to 8.5 with ammonia water, and react with stirring at 20°C for 12 hours.

[0044] (6) Purification of immunogens and antigens: after the coupling reaction, unreacted small molecul...

Embodiment 2

[0052] (1) Dissolve BSA and KLH in PBS at pH 9.5, respectively, at a concentration of 5 mg / mL.

[0053] (2) Dissolve 10 mg of chelating agent ITCBE in 0.1 mol / L, pH 9.5 sodium phosphate buffer solution with a concentration of 20 mg / mL.

[0054] (3) Synthesis of hapten Hg-ITCBE: 1000μL 1mg / LHg 2+ The standard solution was added to 2000 μL of 4.6 mM chelating agent solution, the pH was adjusted to 7.5 with ammonia water, and the reaction was stirred at 25° C. for 24 h.

[0055] (4) Synthesis of the immunogen Hg-ITCBE-KLH: take 3 mg of hapten, add 3 mg of KLH, adjust the pH to 9.0 with ammonia water, and react with stirring at 25° C. for 24 hours.

[0056] (5) Synthesis of the coated antigen Hg-ITCBE-BSA: take 3 mg of hapten, add 3 mg of BSA, adjust the pH to 9.0 with ammonia water, and react with stirring at 25° C. for 24 hours.

[0057] (6) Purification of immunogens and antigens: After the coupling reaction, unreacted small molecular substances were removed by centrifugation...

Embodiment 3

[0069] (1) Dissolve BSA and KLH in PBS at pH 9.5, respectively, at a concentration of 6 mg / mL.

[0070] (2) Dissolve 10 mg of chelating agent ITCBE in 0.1 mol / L, pH 9.5 sodium phosphate buffer solution with a concentration of 20 mg / mL.

[0071] (3) Synthesis of hapten Hg-ITCBE: 1000μL 1mg / LHg 2+ The standard solution was added to 2000 μL of 4.6 mM chelating agent solution, the pH was adjusted to 7.5 with ammonia water, and the reaction was stirred at 25° C. for 36 h.

[0072] (4) Synthesis of the immunogen Hg-ITCBE-KLH: take 3 mg of hapten, add 3 mg of KLH, adjust the pH to 9.0 with ammonia water, and react at 25° C. for 36 h.

[0073] (5) Synthesis of the coated antigen Hg-ITCBE-BSA: take 3 mg of hapten, add 3 mg of BSA, adjust the pH to 9.0 with ammonia water, and react with stirring at 25°C for 36 hours.

[0074] (6) Purification of immunogens and antigens: after the coupling reaction, unreacted small molecular substances were removed by centrifugation using centeicon-30 ...

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Abstract

The invention relates to a monoclonal antibody which can resist mercury chelate. The monoclonal antibody has specificity to the mercury chelate. The mercury chelate is mercury-1-(4-benzyl-L-isothiourea)-ethylenediaminetetraacetic acid-bovine serum albumin-coupled material and mercury-EDTA. The monoclonal antibody has high titer and strong specificity. The invention also provides a method for preparing the monoclonal antibody which can resist mercury chelate and application thereof.

Description

technical field [0001] The invention relates to the technical field of heavy metal immunodetection, in particular to an anti-mercury chelate monoclonal antibody and its preparation method and application. Background technique [0002] Heavy metal pollution has caused serious harm to the ecological environment and human health, especially mercury, which has attracted widespread attention due to its complexity and toxicity. system, skin, central nervous system, kidneys and eyes. As early as the 1950s, after the outbreak of Minamata disease in Kumamoto, Japan, mercury pollution has attracted people's attention. In recent years, with the continuous acceleration of the urbanization process and the rapid improvement of the level of industrialization, the pollution caused by production and living has become increasingly serious and has once again become one of the global hot issues. Therefore, the research on the emission, migration, deposition and control of mercury has become o...

Claims

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Application Information

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IPC IPC(8): C07K16/44C12P21/08G01N33/577
Inventor 王菊芳李志勇黄佳俊王小宁
Owner SOUTH CHINA UNIV OF TECH
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