Preparation of rare earth luminous nanoparticle based on pyridine dicarboxylic acid

A technology of dipicolinic acid and nanoparticles, applied in the direction of luminescent materials, chemical instruments and methods, measuring devices, etc., can solve problems such as unsatisfactory dispersion, no commercial source, complex organic synthesis, etc., to avoid complex organic synthesis, Good water solubility and stability, good water solubility effect

Inactive Publication Date: 2009-07-22
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Since these methods use non-solution homogeneous preparation, the uniformity of the shape of the prepared particles, the controllability of the size, and the dispersion in the aqueous solution are not ideal.
[0007] Chinese Patent Publication No. CN 1493647A, 2004, Yuan Jingli, Tan Mingqian, Ye Zhiqiang, Wang Guilan, a functional nano-rare earth fluorescent particle and its preparation and application, discloses a particle preparation method for wrapping rare earth complexes, the method is to emit light Strong rare earth complexes as the center, using SiO 2 Wrapped into nanoparticles as markers, the prepared particles have good uniformit...

Method used

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  • Preparation of rare earth luminous nanoparticle based on pyridine dicarboxylic acid
  • Preparation of rare earth luminous nanoparticle based on pyridine dicarboxylic acid
  • Preparation of rare earth luminous nanoparticle based on pyridine dicarboxylic acid

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: Preparation of dipicolinic acid-Eu nanoparticles modified by oligonucleotide probes

[0031] Preparation of rare earth complexes: the rare earth Eu 3+ Aqueous solution (Eu 3+ Hydrochloride) and dipicolinic acid solution were mixed and reacted for 10 minutes at a molar ratio of 1:3.

[0032] Preparation of rare earth nanoparticles: Mix cyclohexanone, n-hexanol and Triton X-100 (v / v, 4:1:1) and stir evenly, add an appropriate amount of rare earth complex solution (water phase), and stir fully to form an oil-coated After the water microemulsion, add tetraethyl orthosilicate and concentrated ammonia water, and after stirring for 2 hours, add aminopropyltriethoxysilane (aminosilylating agent), and react the reaction mixture for 20 hours under stirring. After the reaction is completed, the milky white Add an appropriate amount of acetone to the suspension for centrifugal sedimentation, wash with ethanol and water to remove the reaction solution, and centrifuge...

Embodiment 2

[0034] Embodiment 2: Preparation of dipicolinic acid-Tb nanoparticles

[0035] Preparation of rare earth complexes: the rare earth Tb 3+ Aqueous solution (Tb 3+ Nitrate or hydrochloride) and dipicolinic acid solution were mixed and reacted for 10 minutes at a molar ratio of 1:3.

[0036] Preparation of rare earth nanoparticles: Mix cyclohexanone, n-hexanol and Triton X-100 (v / v, 4:1:1) and stir evenly, add an appropriate amount of rare earth complex solution (water phase), and stir fully to form an oil-coated After the water microemulsion, add tetraethyl orthosilicate and concentrated ammonia water, stir for 2 hours, add aminopropyltriethoxysilane (silylating agent), and react the reaction mixture for 20 hours under stirring. After the reaction is completed, it will be suspended in milky white A proper amount of acetone was added to the solution for centrifugal sedimentation, washed with ethanol and water respectively to remove the reaction solution, and centrifuged to obtai...

Embodiment 3

[0037] Embodiment 3: Preparation of dipicolinic acid-Dy nanoparticles

[0038] Preparation of rare earth complexes: the rare earth Dy 3+ Aqueous solution (Dy 3+ Hydrochloride) and dipicolinic acid solution were mixed and reacted for 10 minutes at a molar ratio of 1:3.

[0039] Preparation of rare earth nanoparticles: Mix cyclohexanone, n-hexanol and Triton X-100 (v / v, 4:1:1) and stir evenly, add an appropriate amount of rare earth complex solution (water phase), and stir fully to form an oil-coated After the water microemulsion, add tetraethyl orthosilicate and concentrated ammonia water, stir for 2 hours, add aminopropyltriethoxysilane (silylating agent), and react the reaction mixture for 20 hours under stirring. After the reaction is completed, it will be suspended in milky white A proper amount of acetone was added to the solution for centrifugal sedimentation, washed with ethanol and water respectively to remove the reaction solution, and centrifuged to obtain SiO 2 Th...

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Abstract

The invention relates to a preparation method of rare-earth luminescent nanometer particles based on pyridine dicarboxylic acid. The rare-earth luminescent nanometer particle comprises SiO2 coating luminescent rare-earth composition; the rare-earth composition is produced by ligand pyridine dicarboxylic acid combined with rare-earth ions; and SiO2 nanometer particles with active functional group on the surface are formed by the polymerization reaction of the rare-earth composition in the microemulsion and the silanization. The prepared rare-earth luminescent nanometer particle has high luminous intensity and good water solubility, and can be used for a variety of time-resolved fluorescence analysis and imaging. Compared with the homogeneous rare-earth nanometer particle preparation method, the preparation method has the advantages of low preparation cost and simple method, and can be widely applied.

Description

technical field [0001] The invention relates to a preparation method of rare earth luminescent nanoparticles based on dipicolinic acid, which is mainly used for fluorescence detection and tracing of biomolecules, and belongs to the technical field of preparation of biomolecular nanometer markers. Background technique [0002] Fluorescence detection and imaging technologies are widely used in many fields such as life sciences and medicine. However, various organic dye molecules used in traditional fluorescence analysis are susceptible to interference from various non-specific fluorescence (excitation light, scattered light and analyte background fluorescence ), and the dye molecules are prone to photobleaching and quenching effects, resulting in a decrease in fluorescence intensity. Nanomarkers are a new type of markers developed in recent years. Since nanoparticles are loaded with several fluorescent molecules through surface adsorption or embedding, the fluorescence emissio...

Claims

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Application Information

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IPC IPC(8): C09K11/78G01N33/533
Inventor 陈扬
Owner SOUTHEAST UNIV
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