Peganum harmalaL total alkaloids separation method in peganum harmalaL extract

Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating total alkaloid from an extracting solution of the traditional Chinese medicine, namely Peganum harmala, and belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal: taking the extracting solution of the Peganum harmala; adjusting the pH value of the extracting solution to between 1 and 7; filtering the extracting solution; taking and adding the filtrate to a cation exchange resin column; rinsing the filtrate and removing the impurities; eluting the filtrate with 0.5 to 15 percent acid liquor; collecting eluent; adding alkali to the eluent for neutralization; performing desalting treatment on the mixed solution; and obtaining the Peganum harmala total alkaloid. The method overcomes the defects of low extracting rate, large using level of organic solvent, complicated process, inability of large-scale production, and the like in the prior art and can efficiently separate high purity of the Peganum harmala total alkaloid from the extracting solution of the Peganum harmala.

Description

technical field [0001] The invention relates to a method for extracting and separating active ingredients of traditional Chinese medicines, in particular to a method for extracting and separating total alkaloids of camel pontifex from extracts of traditional Chinese medicine ponchos, belonging to the field of traditional Chinese medicines. Background technique [0002] Alkaloids are a large class of basic nitrogen-containing compounds that widely exist in nature, are active ingredients of many Chinese herbal medicines, and are an important class of substances in nature. The source of Camelia is Peganum harmalaL., a plant of Tribulus terrestris, which is used as medicine with the whole plant and seeds. Also known as bitter bitter vegetable, Artemisia camel, stinky grass, stinky peony, safflower peas, etc., it is a commonly used folk Chinese herbal medicine. Studies have shown that the total alkaloids of camels are its main effective parts, including Harmaline, Harmine, Harma...

AI Technical Summary

Problems solved by technology

Compared with the methods in other reports, the above two methods have improved the purity of alkaloids, and have certain progress, but there are still serious defects: after the two methods use ammonia to alkalize the resin, elute with organic solvent or reflux with chloroform However, due to the low alkalinity of ammonia water and the incomplete dissociation of alkaloids, the transfer rate is still not high; the use of toxic organic solvents such as ether and chloroform has brought hidden dangers to the production operation and the safety of the final preparation The use of organic solvents will bring residues of resin monomer components such as styrene and divinylbenzene into the eluent, which will affect the safety of the preparation; the latter also needs to pour out the resin and put the resin into a Soxhlet extractor The use of diethyl ether for reflux extraction has brought a lot of trouble to large-scale production; at the same time, due to the use of organic solvents in the process, it is also required to increase the ethanol treatment step in the pre-treatment of the resin, and the organic solvent must be recovered later, which increases the process steps and improves the quality of the resin. cost of production