Preparation method of heparin modified gold nano-particles

A technology of gold nanoparticles and heparin, applied in the field of nanodiagnosis, can solve the problems of strong binding force, single structure, insufficient research on gold nanoparticles, etc., and achieve the effect of low cost and good stability

Inactive Publication Date: 2009-07-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, gold nanoparticles are often modified with chemical or biological molecules such as DNA, protein, polymer, dendrimers or functionalized sulfhydryl groups, but research on carbohydrate or sugar-modified gold nanoparticles is not deep enough.
Most of the existing studies use small molecular monosaccharide or disaccharide units to modify gold nanoparticles, with a single structure; or use positive and negative electrostatic adsorption methods to adsorb charged polysaccharides on the surface of nanoparticles.
This electrostatic binding force is not as strong as that of chemical bonds, and is easily affected by ions in solution, making the formed polysaccharide-modified nanoparticles unstable.

Method used

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  • Preparation method of heparin modified gold nano-particles

Examples

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Embodiment 1

[0017] Example 1: Preparation of low molecular weight heparin whose reducing end is an aldehyde group

[0018] 1:1~1:20 of 0.5M H 2 SO 4 and 5.5M NaNO 2 The solution was made up to 5mlHNO 2 solution, then add 1g of heparin, mix well, react at 0-10°C for 0.5-4 hours, shake the solution every 5-10 seconds during the reaction, after the reaction, use 3ml 1:1:1~ 1:10:10 of 1M Na 2 CO 3 、H 2 O, 1M NaHCO 3 neutralize the mixed solution, adjust the pH to 7, and use 500ml-1000ml of 50mM NH 4 HCO 3 The solution was dialyzed three times and freeze-dried to obtain a low molecular weight heparin whose reducing end was an aldehyde group.

Embodiment 2

[0019] Embodiment 2: Preparation of low molecular weight heparin with sulfhydryl group

[0020] 1g of low-molecular-weight heparin whose reducing end is an aldehyde group is dissolved in 10-100ml of 0.2M phosphate buffer (pH7.0), and then 10ml of 0.1-1M mercaptoethylamine solution is added. After stirring evenly, add 1-2g The sodium cyanoborohydride catalyst is reacted at a temperature of 55-65°C for 2-10 hours. After the reaction, the solution is centrifuged, and the supernatant is dialyzed and then freeze-dried to obtain a low molecular weight heparin sample with mercapto groups.

Embodiment 3

[0021] Embodiment 3: Preparation of low molecular weight heparin with sulfhydryl group

[0022] 1g of low-molecular-weight heparin whose reducing end is an aldehyde group is dissolved in 10-100ml of 0.2M phosphate buffer (pH7.0), and then 10ml of 0.1-1M p-mercaptoaniline solution is added. After stirring evenly, add 1-2g The sodium cyanoborohydride catalyst is reacted at a temperature of 55-65°C for 2-10 hours. After the reaction, the solution is centrifuged, and the supernatant is dialyzed and then freeze-dried to obtain a low molecular weight heparin sample with mercapto groups.

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Abstract

The invention belongs to the field of nano diagnosis, in particular to a method for preparing heparin-modified gold nano particles. The method for preparing the heparin-modified gold nano particles is to add low molecular weight heparin into a gold nano particle solution which is reduced by a reducing agent, and obtain the gold nano particles which are modified by the low molecular weight heparin, wherein the low molecular weight heparin is hydrosulphonyl-containing low molecular weight heparin, and the reducing agent is sodium borohydride or sodium citrate. The method adopts the hydrosulphonyl-containing low molecular weight heparin as a modifier to stabilize gold nano particles which are prepared by taking the sodium borohydride or the sodium citrate as the reducing agent, and obtains the gold nano particles with small particle diameter and uniform distribution by controlling the proportion of the hydrosulphonyl-containing low molecular weight heparin and the sodium borohydride or the sodium citrate. Moreover, the method can be applied to detection of proteins or viruses, and has the advantages of quickness and convenience compared with other virus detection methods such as an immunological method and PCR polymerase chain reaction. The invention provides a novel method for industrialized production for quick virus detection.

Description

technical field [0001] The invention belongs to the field of nano-diagnosis, and relates to a method for preparing heparin-modified gold nanoparticles, in particular to a method for preparing heparin-modified gold by using low-molecular-weight heparin with sulfhydryl groups as a modifier and sodium borohydride or sodium citrate as a reducing agent. nanoparticle approach. Background technique [0002] In recent years, with the rise of nanotechnology, surface self-assembly of gold nanoparticles has received extensive attention. Nanoscale gold particles have shown potential application value in many fields due to their unique properties, which has aroused people's strong research interest. Gold nanoparticles are the most stable metal nanoparticles. Nanoscale gold particles have unique, size-related optical, thermal, electrical, magnetic and chemical properties: such as surface plasmon resonance absorption (SPR) , Raman scattering (RS), and high catalytic activity, biocompatibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24C08B37/10
Inventor 陈敬华金坚许正宏万菁
Owner JIANGNAN UNIV
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