Method for separating and purifying flavonoid glycoside monomer from sensitive plant
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A flavone glycoside monomer, separation and purification technology, applied in the field of traditional Chinese medicine, can solve the problems of low efficiency, high production cost, low operation safety and the like
Inactive Publication Date: 2009-08-19
ZHEJIANG FORESTRY UNIVERSITY
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The above methods all have the disadvantages of many operation steps, complex process, long time, low efficiency, high equipment requirements, large amount of solvent, high price and strong toxicity, low operation safety, and high production cost.
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[0026] Now in conjunction with the embodiments of the present invention and activity tests, further illustrate the beneficial effects of the present invention.
[0027] The method of the present invention is: 1) raw material processing: pulverize the whole Mimosa herb into coarse powder, and pass through a 20-40 mesh sieve; 2) extraction: put the Mimosa coarse powder into an extraction container, and put it into ultrasonic extraction Under the condition of 50-60°C, use 6-8 times the amount of 40%-60% aqueous ethanol as a solvent for ultrasonic extraction 2-3 times, each time for 30-60 minutes, suction filter, and combine the filtrates. 3) Concentration under reduced pressure: Concentrate the filtrate in step 2) with a vacuum film at a water bath temperature below 60°C until the filtrate has no alcohol smell, and recover ethanol to obtain a concentrated solution, the concentration of which is equivalent to 1 mL of concentrated solution per g of coarse powder of medicinal materia...
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Abstract
A method for extracting and purifying a flavonoid glycoside monomer from a sensitive plant belongs to the technical field of Chinese traditional medicine. The technical scheme is as following: taking the sensitive plant as a raw material; performing ultrasonic extraction and concentration, and then performing alcohol precipitation to remove impurities and reconcentration; separating and enriching by macroporous resin column chromatography, and then separating by combing a gel resin column chromatography technology and a silica gel column chromatography technology to obtain three monomeric compounds including vitexin, orientin and isoorientin, wherein, the three monomeric compounds have the extraction yield of 1.12%, 2.62% and 2.21% respectively, and the purity of the three is above 95%. The method has the advantages of convenient operation, low cost, high purity, high yield, repetitive recycle of the macroporous resin and the solvent, disuse of toxic solvents in the process, no environmental pollution, easy industrialized production and the like.
Description
technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a method for separating and purifying flavonoid glycoside monomers from Mimosa pudica. Background technique [0002] Mimosa is the whole plant of Mimosa pudica in the leguminous family (Leguminosae), also known as Mimosa, Mimosa, etc. It is mainly distributed in hillside jungles, wetlands, roads, etc. in East China, South China, and Southwest my country. Beside, there are many distributions in Hainan. Mimosa has the effects of clearing heat and diuresis, reducing phlegm and relieving cough, calming the nerves and relieving pain, cooling blood and stopping bleeding. It is mostly used clinically for acute hepatitis, neurasthenia, insomnia, tuberculosis, hemoptysis, hematuria, and herpes zoster. Mimosa mainly contains flavonoids, phenolic acids and other components. Among the more components of Mimosa, vitexin, orientin, and isoorientin have high content and ou...
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