Method for separating and purifying flavonoid glycoside monomer from sensitive plant

A flavonoid glycoside monomer, separation and purification technology, applied in the field of traditional Chinese medicine, can solve the problems of numerous operation steps, strong toxicity, and high price
CN101508711BInactive Publication Date: 2011-07-06ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG FORESTRY UNIVERSITY
Publication Date
2011-07-06
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

A method for extracting and purifying a flavonoid glycoside monomer from a sensitive plant belongs to the technical field of Chinese traditional medicine. The technical scheme is as following: taking the sensitive plant as a raw material; performing ultrasonic extraction and concentration, and then performing alcohol precipitation to remove impurities and reconcentration; separating and enrichingby macroporous resin column chromatography, and then separating by combing a gel resin column chromatography technology and a silica gel column chromatography technology to obtain three monomeric compounds including vitexin, orientin and isoorientin, wherein, the three monomeric compounds have the extraction yield of 1.12%, 2.62% and 2.21% respectively, and the purity of the three is above 95%. The method has the advantages of convenient operation, low cost, high purity, high yield, repetitive recycle of the macroporous resin and the solvent, disuse of toxic solvents in the process, no environmental pollution, easy industrialized production and the like.
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Description

technical field

[0001] The invention belongs to the technical field of traditional Chinese medicines, in particular to a method for separating and purifying flavonoid glycoside monomers from Mimosa pudica. Background technique

[0002] Mimosa is the whole plant of Mimosa pudica in the leguminous family (Leguminosae), also known as Mimosa, Mimosa, etc. It is mainly distributed in hillside jungles, wetlands, roads, etc. in East China, South China, and Southwest my country. Beside, there are many distributions in Hainan. Mimosa has the effects of clearing heat and diuresis, reducing phlegm and relieving cough, calming the nerves and relieving pain, cooling blood and stopping bleeding. It is mostly used clinically for acute hepatitis, neurasthenia, insomnia, tuberculosis, hemoptysis, hematuria, and herpes zoster. Mimosa mainly contains flavonoids, phenolic acids and other components. Among the more components of Mimosa, vitexin, orientin, and isoorientin have high content and ou...

Claims

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