Method for fermenting functional wheat bran
A technology for wheat bran and a production method, applied in the field of fermentation, can solve problems such as high cost and complicated process, and achieve the effects of improving synthesis speed, promoting absorption, and promoting feeding
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0019] The method comprises the following steps: (1) Aspergillus niger obtains Aspergillus niger spores after being cultured on a slope at 28° C. for 65 hours; washes the Aspergillus niger spores with sterile physiological saline to obtain a suspension of Aspergillus niger spores; inoculates the Aspergillus niger spore suspension Put into the primary culture medium and carry out shake flask primary seed culture, the access amount of Aspergillus niger spore suspension is 6% of the primary medium weight, and the component of primary medium is: wheat bran 15%, phosphate diphosphate Potassium hydrogen 3%, the rest is sterile water, cultivated at 28°C for 26 hours to obtain the first-grade seed liquid of Aspergillus niger; the first-grade seed liquid was inserted into the second-level medium for secondary culture in shake flasks, and the first-grade seed liquid The incorporation amount is 0.1% of the weight of the secondary medium, and the components of the secondary medium are: 30%...
Embodiment 2
[0024] The method comprises the following steps: (1) aspergillus niger obtains spores of aspergillus niger after being cultured on a slope at 20° C. for 72 hours, and the slope medium used is a slope medium made from bran hydrolyzate; washes the aspergillus niger spores with sterile physiological saline Obtain the aspergillus niger spore suspension; insert the aspergillus niger spore suspension into the primary medium and carry out shake flask primary seed culture, the insertion amount of the aspergillus niger spore suspension is 0.1% of the primary medium weight, one The components of the grade culture medium are: 40% of wheat bran, 0.05% of potassium chloride, and the rest is sterile water, cultivated at 22°C for 30 hours to obtain the grade 1 seed liquid of Aspergillus niger; The first-grade medium is used for secondary culture in shake flasks. The amount of access of the first-grade seed solution is 0.1% of the weight of the second-grade medium. The components of the second...
Embodiment 3
[0029]The method comprises the following steps: (1) Aspergillus niger obtains Aspergillus niger spores after being cultured on a slope at 30° C. for 40 hours, and the slope medium used is a slope medium made from bran hydrolyzate; washes the Aspergillus niger spores with sterile physiological saline Obtain the Aspergillus niger spore suspension; Insert the Aspergillus niger spore suspension into the first-level medium to carry out shake flask first-level seed culture, and the insertion amount of the Aspergillus niger spore suspension is 0.5% of the first-level medium weight, one The components of the first-grade culture medium are: 20% of wheat bran, 2% of disodium hydrogen phosphate, and the rest is sterile water, cultivated at 30°C for 20 hours to obtain the first-grade seed liquid of Aspergillus niger; put the first-grade seed liquid into The secondary culture medium is carried out in shake flask secondary culture, the access amount of the primary seed solution is 5% of the ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More 