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Freezeproof polypeptide, synthesis, composition containing same and application thereof

A technology of polypeptide composition and antifreeze polypeptide, applied in the biological field, can solve problems such as unfavorable industrialized large-scale production, biological methods are time-consuming and laborious, and cannot repeat experiments, and achieve the effects of excellent antifreeze effect, excellent effect and high yield

Inactive Publication Date: 2012-05-02
北京金迪克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, biological methods are usually used for peptide synthesis, but biological methods are time-consuming and laborious, which is not conducive to large-scale industrial production
In addition, the document "Chemical Synthesis of O-Acetylgalactosaminylated Amino Acids", "Journal of Third Military Medical University", Volume 22, No. 10, pages 958-960, reported a peptide synthesis method. The route is short, but according to the document The method cannot be replicated

Method used

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  • Freezeproof polypeptide, synthesis, composition containing same and application thereof
  • Freezeproof polypeptide, synthesis, composition containing same and application thereof
  • Freezeproof polypeptide, synthesis, composition containing same and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, the preparation of antifreeze peptide

[0060] 1. Main reagents

[0061] Analytical pure acetylgalactosamine (GalNAc), BF 3 .Et 2 O, SnCl 4 , CH 2 Cl 2 , acetic anhydride and chromatographically pure CH 3 CN was purchased from Sigma; Fmoc-Thr-OH, Fmoc-Ala-OH, and Fmoc-Asn(Trt)-OH monomers were purchased from Advanced ChemTech; RinkAmide resin was purchased from Tianjin Nankai Hecheng Technology Co., Ltd.

[0062] 2. Experimental method

[0063] 2.1 Acetylgalactosamine peracetylation:

[0064]

[0065] a SnCl 4 , Ac 2 O, CH 2 Cl 2 Yield: 52%

[0066] Add 1.5g GalNAc, 15ml CH 2 Cl 2 , 15ml acetic anhydride and 120mg SnCl 4 , after reflux reaction at 50°C for 12 hours, it was separated by a normal phase silica gel column, and the eluent was CH 2 Cl 2 . Collect CH 2 Cl 2 The eluate was drained by a rotary evaporator, added ether and stirred until crystallization, and drained to obtain 0.782 g of the product. An appropriate amount of the...

Embodiment 2

[0076] Embodiment 2, antifreeze peptide composite prescription, when composed of antifreeze peptide, glycerin, mannitol, hydroxypropyl methylcellulose and sterile multiple distilled water, can be used for the protection of skin under low temperature environment.

[0077]

[0078] First soak the hydroxypropyl methylcellulose in the above formula in sterile multiple distilled water, and then add the antifreeze peptide and mannitol pre-dissolved in sterile multiple distilled water after it is completely swelled, then add glycerin, stir well That's it.

Embodiment 3

[0079] Embodiment 3, antifreeze peptide compound prescription, by antifreeze peptide, carbomer, glycerin, ethylparaben, triethanolamine appropriate amount (adjust pH to 6~9), sterile multiply distilled water to make hydrogel , can be used for protection of exposed skin in low temperature environment.

[0080]

[0081] Dissolve the carbomer resin in water first, and after it is completely swollen, add glycerin and mix evenly, or pass through a colloid mill, and then add triethanolamine to neutralize until the pH is 6-9 to obtain a transparent gel. When adding triethanolamine, minimize the generation of bubbles. Then add the antifreeze peptide aqueous solution into the above-mentioned gel, stir evenly and divide into packages. This gel is water-soluble, transparent and beautiful in appearance, and has a special delicate and smooth feeling when applied to the skin. It has an excellent coupling effect with the skin and can prevent the skin from frostbite caused by the low temp...

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Abstract

The invention relates to freezeproof polypeptide, synthesis, a composition containing same and the application thereof. In the invention, after threonine carries out glycosylation modification in an improvement mode, fixed-phase polypeptide is synthesized to obtain the freezeproof polypeptide. The freezeproof polypeptide composition is prepared with various components into aqueous solution, an aquagel agent, cream, emulsion, a facemask, a patch, and the like. The preparation can be used for storing cells, bacteria, tissues and organs at low temperature, has anti-inflammatory function for treating wounds, ulcers and burns, can promote the healing of the wounds, increase the healing quality of the wounds and effectively reduce scars. When the freezeproof polypeptide composite composition preparation is used for skin care products, red spots and skin burns caused by irradiation of ultraviolet rays can be reduced, and chilblain, chap and blood traces of exposed skin can be prevented in low-temperature environment.-temperature environment.

Description

technical field [0001] The present invention relates to the biological field, in particular to a polypeptide. In particular, it relates to an antifreeze polypeptide, its synthesis, composition and application containing the polypeptide. Background technique [0002] Antifreeze proteins (AFPs) are a class of proteins that can inhibit the growth of ice crystals, change the shape of ice crystals, and inhibit the recrystallization of ice. Also known as heat hysteresis proteins or temperature hysteresis proteins (thermal hysteresisproteins THPs). Since Devries first discovered it in the blood of a Nototheneniid fish in the Mcmurdo Strait of Antarctica in 1969, so far, antifreeze proteins have been discovered from fish, insects, plants, fungi and bacteria, and their mechanism of action and molecular structure have been studied. in-depth research. At present, the research on antifreeze protein in the field of agriculture is very extensive, and it has attracted more and more atte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K9/00C07K14/00A61K38/14A61K38/16A61K8/64A61P17/02A61Q17/04A61Q19/00A01N1/02C12N1/20
Inventor 唐旭东候云德
Owner 北京金迪克生物技术有限责任公司