Composite microbiological antibiotic and preparation method thereof
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A technology of compound microorganisms and antibiotics, which is applied in the field of food microorganisms, and can solve the problems of ineffective inhibition and killing
Active Publication Date: 2009-09-09
新疆瑞源乳业有限公司
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[0004] As a food antibiotic, lactic acid bacteria bacteriocin is not a broad-spectrum antibacterial, and its antibacterial effect on Gram-positive bacteria is relatively obvious, but its inhibitory effect on Gram-negative bacteria, mold, yeast and other fungi is not obvious
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Embodiment 1
[0046] 1. Preparation process
[0047] 1.1 Preparation of bacteriocin by mixed fermentation of lactic acid bacteria
[0048] 1.1.1 Source of bacteria
[0049] The fermentation strains were purchased from China Industrial Microorganism Culture Collection Center, the names and numbers are: Lactobacillus rhamnosus CICC6141 (A), Lactobacillus acidophilus CICC 6075 (B).
[0050] 1.1.2 Fermentation process
[0051] 1.2.2.1 Preparation of MRS medium
[0052] Peptone 1%, Beef Extract 1%, Yeast Extract 0.5%, K 2 HPO 40.2%, Diamine Citrate 0.2%, Sodium Acetate 0.5%, Glucose 2%, Tween 800.1%, MgSO 4 ·7H 2 O0.058%, MnSO 4 4H 2 O 0.025%, the rest is deionized water, pH6.2-6.4, sterilized at 121°C for 20 minutes.
[0053] 1.2.2.2 Activation of fermentation strains
[0054] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, respectively put Lactobacillus rhamnosus CICC 6141 and Lactobacillus acidophilus CICC 6075 into two ampou...
Embodiment 2
[0074] 1. Preparation process
[0075] 1.1 Preparation of bacteriocin by mixed fermentation of lactic acid bacteria
[0076] 1.1.1 Source of bacteria
[0077] The fermentation strains were purchased from China Industrial Microorganism Culture Collection Center, the names and numbers are: Lactobacillus rhamnosus CICC6141 (A), Lactobacillus acidophilus CICC 6075 (B).
[0078] 1.1.2 Fermentation process
[0079] 1.2.2.1 Preparation of MRS medium
[0080] Peptone 1%, Beef Extract 1%, Yeast Extract 0.5%, K 2 HPO 4 0.2%, Diamine Citrate 0.2%, Sodium Acetate 0.5%, Glucose 2%, Tween 800.1%, MgSO 4 ·7H 2 O0.058%, MnSO 4 4H 2 O 0.025%, the rest is deionized water, pH6.2-6.4, sterilized at 121°C for 20 minutes.
[0081] 1.2.2.2 Activation of fermentation strains
[0082] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, respectively put Lactobacillus rhamnosus CICC 6141 and Lactobacillus acidophilus CICC 6075 into two ampo...
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Abstract
The invention provides a composite microbiological antibiotic and a preparation method thereof. The preparation method comprises the steps of respectively culturing lactobacillus rhamnose (A) and lactobacillus acidophilus (B) in an MRS culture medium for 48 hours at 37 DEG C, centrifuging the fermentation liquid at a high speed, adding solid ammonium sulphate into the supernatant, dissolving the generated precipitate in distilled water, purifying the precipitate by gel chromatography and performing freeze dehydration to obtain lactobacillus bacteriocin, adding water in Aspergillus niger thallus based on a ratio of 1:6, milling and crushing by colloid to form homogenate, heating to 55 DEG C, adding 1.5% of cellulase, 1.5% of pectinase, 2% of beta-dextranase and 2% of acid prolealytic enzyme, and mixing uniformly, maintaining temperature at 55 DEG C and hydrolyzing for 6 hours, killing enzyme for 10 minutes at 100 DEG C, after cooling, taking the centrifugal supernatant and performing freeze dehydration to obtain enzymolysis frozen dry powder (C) of mycelium content, and finally uniformly mixing bacteriocin A, bacteriocin B and C in a weight ratio of (15-20):(18-25):(20-35) to obtain the finished product.
Description
(1) Technical field [0001] The invention relates to a broad-spectrum composite microbial antibiotic and a preparation method thereof, belonging to the technical field of food microorganisms. (2) Background technology [0002] The so-called microbial antibiotics are a type of bactericidal protein or polypeptide substance that is synthesized and secreted into the environment during the metabolic process of microorganisms and has an inhibitory effect on the same species or closely related species. These substances can kill or inhibit other microorganisms in the same or similar habitat. All microorganisms that produce antibiotics have specific immune genes, and the antibiotics produced will not cause harm to the bacteria themselves. With the progress of research, some antibiotics with broad-spectrum antibacterial activity against food spoilage microorganisms and pathogenic microorganisms have been discovered. [0003] The research on lactic acid bacteria antibiotics in our coun...
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