Composite microbiological antibiotic and preparation method thereof
A technology of compound microorganisms and antibiotics, which is applied in the field of food microorganisms, and can solve the problems of ineffective inhibition and killing
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Embodiment 1
[0046] 1. Preparation process
[0047] 1.1 Preparation of bacteriocin by mixed fermentation of lactic acid bacteria
[0048] 1.1.1 Source of bacteria
[0049] The fermentation strains were purchased from China Industrial Microorganism Culture Collection Center, the names and numbers are: Lactobacillus rhamnosus CICC6141 (A), Lactobacillus acidophilus CICC 6075 (B).
[0050] 1.1.2 Fermentation process
[0051] 1.2.2.1 Preparation of MRS medium
[0052] Peptone 1%, Beef Extract 1%, Yeast Extract 0.5%, K 2 HPO 40.2%, Diamine Citrate 0.2%, Sodium Acetate 0.5%, Glucose 2%, Tween 800.1%, MgSO 4 ·7H 2 O0.058%, MnSO 4 4H 2 O 0.025%, the rest is deionized water, pH6.2-6.4, sterilized at 121°C for 20 minutes.
[0053] 1.2.2.2 Activation of fermentation strains
[0054] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, respectively put Lactobacillus rhamnosus CICC 6141 and Lactobacillus acidophilus CICC 6075 into two ampou...
Embodiment 2
[0074] 1. Preparation process
[0075] 1.1 Preparation of bacteriocin by mixed fermentation of lactic acid bacteria
[0076] 1.1.1 Source of bacteria
[0077] The fermentation strains were purchased from China Industrial Microorganism Culture Collection Center, the names and numbers are: Lactobacillus rhamnosus CICC6141 (A), Lactobacillus acidophilus CICC 6075 (B).
[0078] 1.1.2 Fermentation process
[0079] 1.2.2.1 Preparation of MRS medium
[0080] Peptone 1%, Beef Extract 1%, Yeast Extract 0.5%, K 2 HPO 4 0.2%, Diamine Citrate 0.2%, Sodium Acetate 0.5%, Glucose 2%, Tween 800.1%, MgSO 4 ·7H 2 O0.058%, MnSO 4 4H 2 O 0.025%, the rest is deionized water, pH6.2-6.4, sterilized at 121°C for 20 minutes.
[0081] 1.2.2.2 Activation of fermentation strains
[0082] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, respectively put Lactobacillus rhamnosus CICC 6141 and Lactobacillus acidophilus CICC 6075 into two ampo...
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