Microbial fertilizer and preparation method thereof
A microbial fertilizer and microbial fermentation technology, which is applied in the treatment and comprehensive utilization of animal slaughtering industrial wastewater, can solve the problems of blood wastewater to the environment, degradation of pesticide residues, etc., to save pollution control costs, degrade pesticide residues, and solve pollution problems Effect
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manufacture Embodiment 1
[0018] Manufacture Example 1: Process 100 kilograms of pig blood wastewater:
[0019] 1. Strain preparation: Lactobacillus acidophilus (L.acidophilus) CGMCC 1.1854 and Bacillus cereus (Bacillus cereus) CGMCC AS 1.895 were selected as the strains.
[0020] Preparation of seed liquid: culture medium: 75 grams of brown sugar, 15 grams of peptone, dilute to 3000 ml with blood wastewater, sterilize at 115°C for 20 minutes; add the same amount of the above-mentioned activated bacteria after cooling down, control the temperature at 34±1 Cultivate for 48 hours at ℃ to prepare a compound seed solution;
[0021] 2. Fermentation: put 100 kg of fresh blood wastewater into the fermentation device, add 3 liters of compound seed liquid and stir evenly, control the temperature at 34±1°C, during the cultivation process, stir slowly intermittently, cultivate for 48 hours, and test the pH3.8, Fermentation is terminated; after inspection and packaging, it is microbial fertilizer.
manufacture Embodiment 2
[0022] Manufacture Example 2: Process 100 kilograms of pig blood wastewater:
[0023] 1. Strain preparation: Lactobacillus plantarum (L.plantarum) CGMCC AS 1.3, Lactobacillus acidophilus (L.acidophilus) CGMCC 1.1854, and Bacillus subtilis (Bacillus subtilis) CGMCC AS 1.140 were selected as the strains.
[0024] Preparation of seed liquid: culture medium: 100 grams of glucose, 100 grams of yeast extract, dilute to 10 liters with blood wastewater, sterilize at 121°C for 15 minutes; insert the above-mentioned bacteria after cooling down, and control the temperature at 28±1°C for cultivation 36 hours, compound seed solution;
[0025] 2. Fermentation: Put 100 kg of blood wastewater into the fermentation device, add 3 liters of molasses, add 10 liters of compound seed liquid, stir evenly, control the temperature at 28±1°C for 60 hours, test the pH3.5, stop the fermentation, and inspect the packaging Then it is microbial fertilizer.
manufacture Embodiment 3
[0026] Manufacture Example 3: Process 100 kilograms of bovine blood wastewater:
[0027] 1. Strain preparation: Lactobacillus fermentum AS 1.122, Lactobacillus plantarum CGMCC AS 1.3, and Bacillus cereus CGMCC AS 1.895 were selected as the strains.
[0028] Seed preparation: medium: 10 grams of molasses, 6 grams of beef extract, dilute to 200 ml with blood wastewater, sterilize at 121 ° C for 15 minutes; insert the above bacteria after cooling down, control the temperature at 36 ± 1 ° C for 72 hours , compound seed solution;
[0029] 2. Fermentation: Put 100 kg of blood wastewater into the fermentation device, add 2.5 kg of brown sugar, insert the above seed liquid, stir evenly, control the temperature for cultivation at 36±1°C, during the cultivation process, stir intermittently, and cultivate for 72 hours to detect PH3. 4. The fermentation is terminated. Further make microbial fertilizer with embodiment 1.
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