Streptomyces strain and application thereof
A technology of streptomyces and fungi, applied in the direction of application, bacteria, and fungicides, can solve problems that threaten the quality of life of the people, restrict the export of agricultural products, and decline in soil fertility, and achieve significant effects of disease prevention and seedling protection, good prevention and control, The effect of promoting plant growth and increasing production
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Embodiment 1
[0025] Embodiment 1, the isolation and identification of Streptomyces bacterial strain YL04
[0026] 1. Isolation of strains
[0027]Streptomyces strain YL04 is a strain with potential biocontrol function that was discovered in 2004 when the strain was isolated from potato early blight leaves collected from the potato base in Keshan County, Heilongjiang. The specific isolation steps are as follows:
[0028] 1. Cut out the junction of the disease and health of the sample, wash it twice with sterile water, then immerse it in 70% alcohol for 5 seconds for surface disinfection, then rinse it once with sterile water, put it on a sterile absorbent filter paper, and naturally to dry.
[0029] 2. Inoculate the sterilized disease samples on the rye sucrose agar medium, and cultivate them at 20°C for 7 days. After 7 days, it was found that on one of the culture medium, while the mycelia of the early blight fungus grew, another kind of colony was formed, and an obvious bacteriostatic z...
Embodiment 2
[0084] Embodiment 2, culture medium and culture condition screening of bacterial strain YL04
[0085] There are many kinds of actinomycete culture media commonly used, such as Gao’s No. 1 medium, oatmeal agar medium, Chapei’s medium, etc., but for different species of actinomycetes and different culture purposes, the most suitable medium different. In this experiment, 44 kinds of medium were selected as the test medium, and 1-2 kinds of growth and production medium most suitable for YL04 were screened out; at the same time, the most suitable bottling medium was determined when cultivating the strain YL04 for the purpose of production. amount, pH value, shaking speed and incubation time. It provides a reference for further research on the physical and chemical properties, structural characteristics, mechanism of action and industrial fermentation production of antibacterial substances produced by YL04.
[0086] 1. Screening of growth medium
[0087]1. Transfer the strain YL0...
Embodiment 3
[0113] The antibacterial activity of embodiment 3, bacterial strain YL04 (inhibition spectrum determination)
[0114] 1. The inhibitory effect of the metabolic fluid of strain YL04 on pathogenic fungi
[0115] In this experiment, 15 species of plant pathogenic fungi from 13 genera were selected, and the inhibitory effect of YL04 metabolic liquid on pathogenic fungi was detected by the Oxford cup method, so as to clarify the antibacterial and biocontrol effects and control spectrum of YL04 bacteria and metabolites. It provides a basis for the further development and field application of the strain.
[0116] The pathogenic fungi tested are as follows: Pythium aphanidermatum, Phytophthora melonis, Phytophthora copsici, Geaumanomyces graminis, Valsa mali , Rhizoctonia solani, Rhizoctonia cerealisbv, Pyricularia grisea, Verticillium dahliae, Alternaria solani, Exserohilum turcicum, Bipolaris maydis, Fusarium graminearum, Fusarium oxysporum f.sp. vasinfectum, Botrytiscinerea. The...
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