Arctic bacteria strain for highly efficiently degrading crude oil and application thereof

A crude oil and strain technology, applied in bacteria, microorganism-based methods, and methods using microorganisms, etc., to achieve the effects of high degradation efficiency, easy cultivation and reproduction, and strong degradation ability.

Inactive Publication Date: 2009-09-16
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Generally, mesophilic bacteria are used for bioremediation at room temperature. However, in the vast northern regions of our country, the temperature will v

Method used

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  • Arctic bacteria strain for highly efficiently degrading crude oil and application thereof
  • Arctic bacteria strain for highly efficiently degrading crude oil and application thereof
  • Arctic bacteria strain for highly efficiently degrading crude oil and application thereof

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Experimental program
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Effect test

specific Embodiment 1

[0023] This strain has the characteristics of degrading crude oil and using it as the sole carbon and energy source. After activating the preserved strain for 3 days, inoculate it into a shaker flask containing 50mL of medium and 2‰ crude oil. Under the condition of 5°C, the shaker speed is 150r / min, and shake for 5 days to degrade the culture of crude oil by the strain. The liquid was extracted by n-hexane, and the GC-MS analysis showed that after degradation by strain P29, compared with the control, the degradation rate of 5 pairs of crude oil reached 41%.

specific Embodiment 2

[0024] Transfer the strains stored on the slant of nutrient agar to a mixture containing 5g / L peptone, 1g / L yeast powder, fresh water and seawater at a ratio of 2:1, MgSO 4 ·7H 2 O 0.025g / L, NaH 2 PO 4 0.5g / L, K 2 HPO 4 ·3H 2 O 1.0g / L; Agar 13~15g / L; Tween 802mL; On plate medium with pH6.0-8.0, streak culture. After culturing for 3 days at a temperature of 8°C, it was found that fuzzy halos appeared around the colonies. The above results show that there is a good correlation between the microbial degradation ability of oil and the production of lipase, which is consistent with the research results of microbial ecology and oil degradation in oil sewage irrigation areas. This study showed that Pseudoalteromonas sp. has lipolytic activity. All kinds of dominant microorganisms isolated from the oil-contaminated soil treatment system have lipolytic activity, and the strains with lipolytic activity have the ability to degrade petroleum hydrocarbons.

specific Embodiment 3

[0025] Get the soil (organic matter content 3.85%) polluted by crude oil in Shengli Oilfield, every gram contains 10mg of crude oil, join it in the inorganic salt culture solution (soil-water mass ratio=1:10), inoculate OD by 2% (v / v) 550nm =2.0 bacterial suspension, cultured with shaking at 5°C on a shaker (150r / min). Samples were taken at different times, the crude oil in the water-soil two-phase was extracted with n-hexane, the concentration of crude oil was measured by GC-MS, and the residual rate was calculated. The results showed that under the above conditions, the degradation rate of crude oil could reach 60% after culturing for 10 days.

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Abstract

The invention provides an arctic bacteria strain P29 for highly efficiently degrading crude oil, which is characterized in that the arctic bacterial strain belongs to Pseudoal teromonas sp., and has the preservation number CGMCC 2913; the bacterial colony is round, the diameter is about between 1.0 and 3.0mm, the edges are smooth, the surface is upheaved, moist and has a smooth upheaval, and the bacterial colony is not transparent and is straw yellow; the thalli is bacillus, the size is (0.8 mu m-1.1 mu m)*(1.5 mu m-3.1 mu m), and the suitable growing conditions are that: the pH is between 8.0 and 8.5 and the temperature is between 5 and 15 DEG C; and at a shaking speed more than 100 r/min, the arctic bacteria strain P29 can grow quickly by taking the crude oil as a unique carbon source and energy at a low temperature (5 DEG C). The bacteria strain has function of degrading various compositions of the crude oil, and has strong capacity of degrading the crude oil, high degradation efficiency and easy culture and reproduction at the low temperature.

Description

technical field [0001] The invention belongs to the technical field of low-temperature biological treatment of pollutants, and in particular relates to an arctic psychrogenic bacterium (Pseudoal teromonassp. P29) that efficiently degrades crude oil, and also relates to an application method for degrading crude oil pollutants by the strain. The strain is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Strains, the address is Datun Road, Chaoyang District, Beijing, the Institute of Microbiology, Chinese Academy of Sciences, the preservation date is February 13, 2009, the preservation number is CGMCC2913, and the classification is named Pseudoalteromonas (Pseudoal teromonas sp.). Background technique [0002] During the process of exploitation, transportation, storage, and processing, oil is discharged into farmland, groundwater, and ocean due to accidents or improper management, polluting the environment, destroying the ecolo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/26C02F3/34B09C1/10C12R1/01C02F101/32
Inventor 林学政杜宁丛柏林沈继红
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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