Fast check reagent of colon bacillus 0157 causing food poisoning

Abstract

The invention relates to a fast check reagent of colon bacillus 0157 causing food poisoning, in particular to a formula of a fast check reagent of colon bacillus 0157 causing enterorrhagia caused by food poisoning and a preparation method thereof. The invention breaks through a traditional sectional check means and adds specific colon bacillus 0157 polyvalent and monovalent serum in culture enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value, has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines and has an obvious advantage in the application of rapidly identifying and diagnosing colon bacillus 0157 which is a food poisoning pathogenic bacterium.

Description

technical field [0001] The invention relates to a rapid detection reagent for food poisoning caused by Escherichia coli 0157. Especially the most common ones have strong viability in the external environment, can grow at 44°C, are very resistant to heat, dryness, and acid environments (pH2.5), and sometimes can resist radiation and antibiotics. They can be used in water, milk and meat. It can survive in food for several months, and what's worse is that a very low dose of Escherichia coli 0157:H7 can cause disease. It is a quick test reagent for Escherichia coli 0157 that reproduces rapidly at the optimum temperature. The normal digestive tract is populated by numerous bacteria competing for living space and nutrients. In this environment, it usually takes thousands of Salmonella to cause disease, but the minimum infectious dose of Escherichia coli 0157:H7 is less than 50, which is an extremely small number for bacteria. Escherichia coli 0157 food poisoning is mostly caused b...

AI Technical Summary

Problems solved by technology

The whole detection process takes a long time, the traditional biochemical identification test cycle is long, the operation is cumbersome, generally takes 5-6 days, the sensitivity is not high, and the quality of the reagents cannot be guaranteed, which seriously affects the confirmation of pathogenic bacteria and the selection on the medium for selective isolation and culture. Suspicious colonies need experience, and there is a possibility of missed detection, which brings great inconvenience to rapid and accurate diagnosis and rescue of patients. There are obvious advantages in the application of