Method for extracting tea polyphenol from glede tea
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A technology of eagle tea and tea polyphenols, which is applied in the fields of anti-toxins, metabolic diseases, organic chemistry, etc., can solve the problems of low yield of tea polyphenols, high cost of membrane filtration, difficult recovery, etc., and achieve good adsorption effect, good Ease effect, easy regeneration effect
Inactive Publication Date: 2009-11-11
HEFEI UNIV OF TECH
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Although the process is simple, the yield of tea polyphenols is not high (less than 20%) due to rough extraction with hot water; and the cost of the filter membrane used in the coarse filtration and nanofiltration process is relatively high, and it is not easy to recycle
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Embodiment 1
[0027] see figure 1 , the operation steps of extracting tea polyphenols from eagle tea are as follows:
[0028] 1. Preparation of eagle tea powder
[0029] The raw material is Ningguo eagle tea. Sensory indicators: normal color and fragrance, no mildew; physical and chemical indicators: tea polyphenol content ≥ 16%, moisture ≤ 8%; hygienic indicators: pesticide residues ≤ 10mg / kg, heavy metals ≤ 20mg / kg. The Ningguo eagle tea was baked at 60°C to constant weight, pulverized by a pulverizer, and passed through a 60-mesh sieve to obtain eagle tea powder.
[0030] 2. Reflux crude extraction of tea polyphenols in Eagle tea
[0031] Weigh 10g of eagle tea powder, add 200ml of 80% ethanol, boil at 100°C for 30 minutes, filter with suction, add 100ml of 80% ethanol to the filter residue, boil at 100°C for 20 minutes, filter with suction, then add 80% ethanol to the filter residue 100ml, reflux at 100°C for 10min, filter with suction, discard the filter residue, collect and combin...
Embodiment 2
[0047] 1. Preparation of eagle tea powder
[0048] The operation is the same as step 1 in Example 1.
[0049] 2. Reflux crude extraction of tea polyphenols in Eagle tea
[0050]Weigh 5g of eagle tea powder, add 100ml of 80% ethanol, boil at 100°C for 30 minutes, filter with suction, add 50ml of 80% ethanol to the filter residue, boil at 100°C for 20 minutes, filter with suction, and then add 80% Ethanol 50ml, reflux at 100°C for 10min, filter with suction, discard the filter residue, collect and combine the three filtrates to obtain the crude extract. The crude extraction rate of tea polyphenols was 28.83%.
[0051] 3. Centrifugation and supernatant treatment
[0052] The pH of the crude extract was adjusted to 2 with dilute hydrochloric acid, and centrifuged at 12000 r / min for 10 min to obtain a clear supernatant. The yield of tea polyphenols in the supernatant was 92.8%. The concentration of the supernatant was diluted to 2 mg / ml with deionized water, and the pH value wa...
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Abstract
The invention relates to a method for extracting tea polyphenol from glede tea, comprising the following process flows: glede tea is dried and crushed, and is screened by a 60-mesh sieve, refluxed and extracted by 80% ethanol, and pH is adjusted to be 2; then glede tea is treated by high-speed centrifugation, supernatant fluid treatment, polyamide absorption, elution, concentration, refrigeration and drying in vacuum, so as to obtain pure tea polyphenol. 80% ethanol is used to crudely extract the tea polyphenol of glede tea, and extraction rate is relatively higher than hot-water extraction rate; the pH of crude extract is adjusted to be 2, and pigment, caffeine and large-molecule substance are separated in advance by high-speed centrifugation; de-ionized water is used to adjust the concentration of the supernatant fluid to ensure low ethanol content in the supernatant fluid, which is convenient for follow-up 80% ethanol to elute tea polyphenol; polyamide has strong adsorbability to tea polyphenol, and has low cost and easy regeneration; the elution rate of 80% ethanol which is used as main eluent can reach 97.2%, and ethanol has no pollution and is safe and easy to recover. The method has simple technical process and high tea polyphenol extraction rate, and EGCG content in the pure product is higher than that in common green tea. The method has the advantages of low production cost and low energy consumption.
Description
technical field [0001] The invention relates to a method for extracting tea polyphenols from eagle tea. Background technique [0002] Eagle tea, also known as "white tea", is a plant substitute tea that has been drunk by ethnic minorities in southern my country for a long time. It is rich in resources in the southern Anhui mountainous area (Ningguo) and has a long history of folk drinking. Eagle tea is made from the young shoots and leaves of Litseacoreana Levl.Var.Lanuginose, a plant of the genus Lauraceae, and is made by a simple process (steaming or boiling water blanching and then drying or roasting). The finished drink has the functions of detoxifying and reducing swelling, improving eyesight, improving thinking, relieving exterior and preventing heatstroke, and has no toxic and side effects. It is a plant with the same origin as medicine and food. Tea polyphenols are not only a new type of natural antioxidant, but also have obvious pharmacological functions such as an...
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