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High concentration fermentation process of 2-keto-D-gluconic acid mixed salt

A technology of gluconic acid and fermentation process, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of easy crystallization of 2-keto-D-gluconate calcium salt, and achieve the effect of improving quality and eliminating emissions

Inactive Publication Date: 2009-11-11
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a high-concentration fermentation process of 2-keto-D-gluconate mixed salt, on the basis of not affecting the fermentation conversion rate, solve the problem of 2-keto-D-gluconate calcium in the fermentation liquid The problem of easy crystallization of salt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Adopt 10 liters of self-control fermentation tanks, insert 350ml of Pseudomonas putida liquid seed solution into 4 liters of fermentation basal medium containing 180g / L glucose, 10g / L corn steep liquor, 40g / L light calcium carbonate, start When the pH was 6.8, the calcium carbonate was exhausted after 20 hours of fermentation, and the sodium carbonate solution with a concentration of 220g / L was started to be fed, and the pH of the fermentation process was controlled at 5.2. After 24 hours of fermentation, 400 g / L of glucose solution was added to control the total sugar concentration at the end of the fermentation broth to 230 g / L until the sugar concentration in the fermentation broth was lower than 2 g / L, and the fermentation ended. During the entire fermentation process, the temperature is controlled at 31°C, the tank pressure is 0.05Mpa, and the rotation speed and ventilation are adjusted to ensure that the dissolved oxygen is 25%. The concentration of 2-keto-D-gluco...

Embodiment 2

[0011] Adopt 100 liters of self-control fermentation tanks, insert 4 liters of Pseudomonas fluorescens liquid seed solution into 45 liters of fermentation basal medium that contains 180g / L of glucose, 12g / L corn steep liquor, and 45g / L light calcium carbonate. The initial pH was 6.7, and the calcium carbonate was exhausted in 23 hours of fermentation, so a sodium carbonate solution with a concentration of 240g / L was started to be added, and the pH of the fermentation process was controlled at 5.2. After 24 hours of fermentation, 450g / L of glucose solution was added to control the total sugar concentration at the end of the fermentation broth to 250g / L until the sugar concentration in the fermentation broth was lower than 2g / L, and the fermentation ended. During the entire fermentation process, the temperature was controlled at 29°C, the tank pressure was 0.05Mpa, and the rotation speed and ventilation were adjusted to ensure that the dissolved oxygen was 28%. The concentration...

Embodiment 3

[0013] Adopt 1000 liters of self-control fermentation tanks, insert 38 liters of Pseudomonas fluorescens liquid seed solution into 380 liters of fermentation base medium that contains 180g / L glucose, 10g / L corn steep liquor, and 48g / L light calcium carbonate. The initial pH was 6.9, and the calcium carbonate was exhausted in 25 hours of fermentation, and the sodium hydroxide solution with a concentration of 260g / L was started to be fed, and the pH of the fermentation process was controlled at 5.2. After 24 hours of fermentation, 500 g / L of glucose solution was added to control the total sugar concentration at the end of the fermentation broth to 260 g / L until the sugar concentration in the fermentation broth was lower than 2 g / L, and the fermentation ended. During the entire fermentation process, the temperature is controlled at 31°C, the tank pressure is 0.05Mpa, and the rotation speed and ventilation are adjusted to ensure that the dissolved oxygen is 32%. The concentration ...

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Abstract

The invention discloses a high concentration fermentation process of 2-keto-D-gluconic acid mixed salt, comprising the following steps: adding the culture medium which contains glucose, corn steep liquor and light calcium carbonate to a fermentation vessel; introducing the bacteria seeding liquid; adding sodium carbonate solution or sodium hydroxide solution in a fed-batch way when the light calcium carbonate in the fermentation vessel runs out; starting to add the glucose solution with the concentration of 300g / L to 500g / L in batches or in fed-batch way when the concentration of the glucose solution in the fermentation culture medium drops to 20g / L to 100g / L; controlling the gross sugar concentration of the fermentation fluid terminal point to 150g / L to 300g / L until the remaining sugar concentration is below 2g / L and the fermentation finishes. The invention solves the problem that the 2-keto-D-calcium gluconate salt is easy to be crystallized, improves the quality of D-iso ascorbic acid and the salt finished products thereof, and puts the discharge of solid waste calcium sulphate waste residue to an end.

Description

technical field [0001] The invention relates to a preparation method of D-isoascorbic acid and its salts, in particular to a high-concentration fermentation process of 2-keto-D-gluconic acid mixed salt. Background technique [0002] 2-Keto-D-gluconic acid is the precursor for the production of D-erythorbic acid and its salts (such as D-erythorbic acid sodium), D-erythorbic acid and its salts can be used as food antioxidant, antiseptic, fresh-keeping, auxiliary Toner. At present, domestic and foreign industries adopt the known indirect fermentation method to produce D-erythorbic acid and its salts. The process is as follows: firstly, the glucose in the bacterial conversion fermentation medium is used to be 2-keto-D-glucose calcium (fermentation process) Light calcium carbonate is used as the neutralizing agent in the medium), and the fermented liquid is purified by sulfuric acid acidification, centrifugation (filtering), etc. to remove calcium ions and other impurities in th...

Claims

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Application Information

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IPC IPC(8): C12P7/58C12R1/39C12R1/40C12R1/43C12R1/06C12R1/18
Inventor 刘杰黄建明李建洲肖江峰张华峰王志伟
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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