The invention relates to isolated polynucleotides from
Corynebacterium glutamicum which are useful in the
regulation of gene expression. In particular, the invention relates to isolated polynucleotides comprising C.glutamicum promoters which may be used to regulate, i.e., either increase or decrease,
gene expression. In certain embodiments, isolated
promoter sequences of the present invention regulate
gene expression through the use of exogenous or endogenous induction. The invention further provides recombinant vectors and recombinant cells comprising isolated polynucleotides of the present invention, preferably in operable association with
heterologous genes. Also provided are methods of regulating bacterial
gene expression comprising growth of a recombinant
cell of the present invention. In particular, the present invention provides methods to regulate genes involved in
amino acid production comprising growth of a recombinant
cell of the present invention. In certain embodiments, the present invention provides methods of regulating
gene expression in
bacteria, particularly
Corynebacterium species, especially of the
genus Corynebacterium, comprising
fermentation growth of a recombinant
cell of the present invention, where
metabolite concentrations, temperature, or
oxygen levels are manipulated to regulate
gene expression.